Anthony A. Hyman
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Condensation of Sidekick at tricellular junctions organizes mechanical forces for cell-cell adhesion remodeling Open
Summary Epithelial morphogenesis relies on coordinated multicellular movements regulated by adhesion and force-generating molecules. It remains elusive how cells ensure organization of these molecules in space and time. Combining in vitro …
SUMO2/3 conjugation of TDP-43 protects against aggregation Open
Cytosolic aggregation of the RNA binding protein TDP-43 (transactive response DNA-binding protein 43) is a hallmark of amyotrophic lateral sclerosis and frontotemporal dementia. Here, we report that during oxidative stress, TDP-43 becomes …
inPhase — A simple, accurate and fast approach to determine phase diagrams of protein condensates Open
Protein phase separation has become a widely studied phenomena in biology with implications in cell metabolism and disease. The study of phase separating proteins often relies on the precise determination of their phase diagrams. These pha…
View article: Biomolecular condensates sustain pH gradients at equilibrium through charge neutralisation
Biomolecular condensates sustain pH gradients at equilibrium through charge neutralisation Open
Electrochemical gradients are essential to the functioning of cells and form across membranes using active transporters. Here, we show in contrast that condensed biomolecular systems sustain significant pH gradients without any external en…
View article: Actin polymerization counteracts prewetting of N-WASP on supported lipid bilayers
Actin polymerization counteracts prewetting of N-WASP on supported lipid bilayers Open
Cortical condensates, transient punctate-like structures rich in actin and the actin nucleation pathway member N-WASP, form during activation of the actin cortex in the C. elegans oocyte. Their emergence and spontaneous dissolution is link…
View article: Local and dynamic regulation of neuronal glycolysis in vivo
Local and dynamic regulation of neuronal glycolysis in vivo Open
Energy metabolism supports neuronal function. While it is well established that changes in energy metabolism underpin brain plasticity and function, less is known about how individual neurons modulate their metabolic states to meet varying…
Different Low-complexity Regions of SFPQ Play Distinct Roles in the Formation of Biomolecular Condensates Open
Demixing of proteins and nucleic acids into condensed liquid phases is rapidly emerging as a ubiquitous mechanism underlying the complex spatiotemporal organisation of molecules within the cell. Long disordered regions of low sequence comp…
View article: Local and dynamic regulation of neuronal glycolysis <i>in vivo</i>
Local and dynamic regulation of neuronal glycolysis <i>in vivo</i> Open
Energy metabolism supports neuronal function. While it is well established that changes in energy metabolism underpin brain plasticity and function, less is known about how individual neurons modulate their metabolic states to meet varying…
View article: Glutamate helps unmask the differences in driving forces for phase separation versus clustering of FET family proteins in sub-saturated solutions
Glutamate helps unmask the differences in driving forces for phase separation versus clustering of FET family proteins in sub-saturated solutions Open
Multivalent proteins undergo coupled segregative and associative phase transitions. Phase separation, a segregative transition, is driven by macromolecular solubility, and this leads to coexisting phases above system-specific saturation co…
View article: Assembly of tight junction belts by surface condensation and actin elongation
Assembly of tight junction belts by surface condensation and actin elongation Open
Formation of biomolecular condensates via phase separation enables compartmentation of many cellular processes. However, how cells can control condensation at specific locations to create complex cellular structures remains poorly understo…
View article: Quantifying collective interactions in biomolecular phase separation
Quantifying collective interactions in biomolecular phase separation Open
Biomolecular phase separation plays a pivotal role in governing critical biological functions and arises from the collective interactions of large numbers of molecules. Characterising the underlying collective interactions of phase separat…
Supplementary Figures 1-3 from Tpx2 Controls Spindle Integrity, Genome Stability, and Tumor Development Open
PDF file - 224K, Supplemental data includes three figures with a detailed description of the cell cycle characteristics of Tpx2(+/-) cells, quantification of tubulin staining in Tpx2-null embryonic cells and levels of Tpx2 mRNA levels in d…
Data from Tpx2 Controls Spindle Integrity, Genome Stability, and Tumor Development Open
Tpx2 is a microtubule-associated protein that activates the cell-cycle kinase Aurora A and regulates the mitotic spindle. Overexpression of Tpx2 is associated with the development of different human tumors and strongly correlates with chro…
Supplementary Figures 1-3 from Tpx2 Controls Spindle Integrity, Genome Stability, and Tumor Development Open
PDF file - 224K, Supplemental data includes three figures with a detailed description of the cell cycle characteristics of Tpx2(+/-) cells, quantification of tubulin staining in Tpx2-null embryonic cells and levels of Tpx2 mRNA levels in d…
Data from Tpx2 Controls Spindle Integrity, Genome Stability, and Tumor Development Open
Tpx2 is a microtubule-associated protein that activates the cell-cycle kinase Aurora A and regulates the mitotic spindle. Overexpression of Tpx2 is associated with the development of different human tumors and strongly correlates with chro…
Different low-complexity regions of SFPQ play distinct roles in the formation of biomolecular condensates Open
Demixing of proteins and nucleic acids into condensed liquid phases is rapidly emerging as a ubiquitous mechanism governing the organisation of molecules within the cell. Long disordered low complexity regions (LCRs) are a common feature o…
View article: A conserved and tunable mechanism for the temperature-controlled condensation of the translation factor Ded1p
A conserved and tunable mechanism for the temperature-controlled condensation of the translation factor Ded1p Open
Heat shock promotes the assembly of translation factors into condensates to facilitate the production of stress-protective proteins. How translation factors detect heat and assemble into condensates is not well understood. Here, we investi…
The path to condensates—19th Wiley Prize in Biomedical Sciences laureates share their discovery stories Open
Preface by Prof. Titia de Lange, Laboratory for Cell Biology and Genetics, The Rockefeller University, New York, NY 10065, USA The 19th Annual Wiley Prize in Biomedical Sciences celebrated a breakthrough in cell biology: how membrane‐less …
View article: SAMHD1 controls innate immunity by regulating condensation of immunogenic self RNA
SAMHD1 controls innate immunity by regulating condensation of immunogenic self RNA Open
Recognition of pathogen-derived foreign nucleic acids is central to innate immune defense. This requires discrimination between structurally highly similar self and nonself nucleic acids to avoid aberrant inflammatory responses as in the a…
View article: Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions
Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions Open
Macromolecular phase separation is thought to be one of the processes that drives the formation of membraneless biomolecular condensates in cells. The dynamics of phase separation are thought to follow the tenets of classical nucleation th…
View article: Adaptation to environmental temperature in divergent clades of the nematode<i>Pristionchus pacificus</i>
Adaptation to environmental temperature in divergent clades of the nematode<i>Pristionchus pacificus</i> Open
Because of ongoing climate change, populations of organisms are being subjected to stressful temperatures more often. This is especially problematic for ectothermic organisms, which are likely to be more sensitive to changes in temperature…
View article: Phase separating RNA binding proteins form heterogeneous distributions of clusters in subsaturated solutions
Phase separating RNA binding proteins form heterogeneous distributions of clusters in subsaturated solutions Open
Macromolecular phase separation is thought to be one of the processes that drives the formation of membraneless biomolecular condensates in cells. The dynamics of phase separation, especially at low endogenous concentrations found in cells…
View article: Glycine-Rich Peptides from FUS Have an Intrinsic Ability to Self-Assemble into Fibers and Networked Fibrils
Glycine-Rich Peptides from FUS Have an Intrinsic Ability to Self-Assemble into Fibers and Networked Fibrils Open
Glycine-rich regions feature prominently in intrinsically disordered regions (IDRs) of proteins that drive phase separation and the regulated formation of membraneless biomolecular condensates. Interestingly, the Gly-rich IDRs seldom featu…
Quantitative theory for the diffusive dynamics of liquid condensates Open
Key processes of biological condensates are diffusion and material exchange with their environment. Experimentally, diffusive dynamics are typically probed via fluorescent labels. However, to date, a physics-based, quantitative framework f…
View article: Local thermodynamics govern formation and dissolution of <i>Caenorhabditis</i> elegans P granule condensates
Local thermodynamics govern formation and dissolution of <i>Caenorhabditis</i> elegans P granule condensates Open
Significance Living cells rely on a continuous flux of energy to spatially organize biochemical processes. It remains unclear whether cells can achieve this spatial organization via thermodynamic principles. Here, we report the striking be…
Author response: Quantitative theory for the diffusive dynamics of liquid condensates Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Appendix 1 Appendix 2 Data availability References Decision letter Author response Article and author information Metrics Abstract Key processes of bio…