A. G. Thomas
YOU?
Author Swipe
View article: A Long QT Mutation Substitutes Cholesterol for Phosphatidylinositol-4,5-Bisphosphate in KCNQ1 Channel Regulation
A Long QT Mutation Substitutes Cholesterol for Phosphatidylinositol-4,5-Bisphosphate in KCNQ1 Channel Regulation Open
We show for the first time that a mutation may shift the channel interaction with PIP2 to a preference for cholesterol. This de novo interaction wanes the sensitivity to PIP2 variations, showing that a mutated channel with a decreased affi…
View article: The Role of Proline in the Membrane Re-entrant Helix of Caveolin-1
The Role of Proline in the Membrane Re-entrant Helix of Caveolin-1 Open
Caveolin-1 has a segment of hydrophobic amino acids comprising approximately residues 103-122. We have performed an in silico analysis of the conformational preference of this segment of caveolin-1 using PepLook. We find that there is one …
View article: Large Changes in the CRAC Segment of gp41 of HIV Do Not Destroy Fusion Activity if the Segment Interacts with Cholesterol
Large Changes in the CRAC Segment of gp41 of HIV Do Not Destroy Fusion Activity if the Segment Interacts with Cholesterol Open
The membrane-proximal external region (MPER) of the gp41 fusion protein of HIV is highly conserved among isolates of this virus and is considered a target for vaccine development. This region also appears to play a role in membrane fusion …
View article: Peptides As Drugs: Myth Or Reality?
Peptides As Drugs: Myth Or Reality? Open
peer reviewed
View article: Juxtamembrane Protein Segments that Contribute to Recruitment of Cholesterol into Domains
Juxtamembrane Protein Segments that Contribute to Recruitment of Cholesterol into Domains Open
We investigated the properties of several peptides with sequences related to LWYIK, a segment found in the gp41 protein of HIV and believed to play a role in sequestering this protein to a cholesterol-rich domain in the membrane. This segm…
View article: Fusogenic Alzheimer's peptide fragment Aβ (29–42) in interaction with lipid bilayers: Secondary structure, dynamics, and specific interaction with phosphatidyl ethanolamine polar heads as revealed by solid‐state NMR
Fusogenic Alzheimer's peptide fragment Aβ (29–42) in interaction with lipid bilayers: Secondary structure, dynamics, and specific interaction with phosphatidyl ethanolamine polar heads as revealed by solid‐state NMR Open
The interaction of the native Alzheimer's peptide C‐terminal fragment Aβ (29–42), and two mutants (G33A and G37A) with neutral lipid bilayers made of POPC and POPE in a 9:1 molar ratio was investigated by solid‐state NMR. This fragment and…
View article: Functional Analysis of the Cell Division Protein FtsW of <i>Escherichia coli</i>
Functional Analysis of the Cell Division Protein FtsW of <i>Escherichia coli</i> Open
Site-directed mutagenesis experiments combined with fluorescence microscopy shed light on the role of Escherichia coli FtsW, a membrane protein belonging to the SEDS family that is involved in peptidoglycan assembly during cell elongation,…
View article: Distribution of Hydrophobic Residues Is Crucial for the Fusogenic Properties of the Ebola Virus GP2 Fusion Peptide
Distribution of Hydrophobic Residues Is Crucial for the Fusogenic Properties of the Ebola Virus GP2 Fusion Peptide Open
The lipid-destabilizing properties of the N-terminal domain of the GP2 of Ebola virus were investigated. Our results suggest that the domain of Ebola virus needed for fusion is shorter than that previously reported. The fusogenic propertie…
View article: Les liposomes : description, fabrication et applications
Les liposomes : description, fabrication et applications Open
Liposomes: description, preparation and applications. Liposomes are spherical vesicles with a diameter ranging from 20 nm to a few thousands nm. These vesicles consist of one or several bilayers which separate an intravesicular from an ext…
View article: Revisiting the Ramachandran plot: Hard‐sphere repulsion, electrostatics, and H‐bonding in the α‐helix
Revisiting the Ramachandran plot: Hard‐sphere repulsion, electrostatics, and H‐bonding in the α‐helix Open
What determines the shape of the allowed regions in the Ramachandran plot? Although Ramachandran explained these regions in terms of 1–4 hard‐sphere repulsions, there are discrepancies with the data where, in particular, the α R , α L , an…
View article: Analysis of accessible surface of residues in proteins
Analysis of accessible surface of residues in proteins Open
We analyzed the total, hydrophobic, and hydrophilic accessible surfaces (ASAs) of residues from a nonredundant bank of 587 3D structure proteins. In an extended fold, residues are classified into three families with respect to their hydrop…
View article: Lipid-interacting properties of the N-terminal domain of human apolipoprotein C-III
Lipid-interacting properties of the N-terminal domain of human apolipoprotein C-III Open
The lipid-interacting properties of the N-terminal domain of human apolipoprotein C-III (apo C-III) were investigated. By molecular modeling, we predicted that the 6-20 fragment of apo C-III is obliquely orientated at the lipid/water inter…
View article: Interaction between the N-terminal Domain of Gastric H,K-ATPase and the Spectrin Binding Domain of Ankyrin III
Interaction between the N-terminal Domain of Gastric H,K-ATPase and the Spectrin Binding Domain of Ankyrin III Open
We screened a cDNA bank of rabbit gastric fundic mucosa by two-hybrid assays looking for binding partners of the N-terminal domain of the rabbit gastric H,K-ATPase. We extracted five clones sharing more than 90% sequence identity. The long…
View article: The solvation concept approached by lipophily
The solvation concept approached by lipophily Open
peer reviewed
View article: The structural and functional organization of H‐NS‐like proteins is evolutionarily conserved in Gram‐negative bacteria
The structural and functional organization of H‐NS‐like proteins is evolutionarily conserved in Gram‐negative bacteria Open
The structural gene of the H‐NS protein, a global regulator of bacterial metabolism, has been identified in the group of enterobacteria as well as in closely related bacteria, such as Erwinia chrysanthemi and Haemophilus influenzae . Isola…