Shiro Kominami
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View article: Retinoic Acid Stimulates 17β-Estradiol and Testosterone Synthesis in Rat Hippocampal Slice Cultures
Retinoic Acid Stimulates 17β-Estradiol and Testosterone Synthesis in Rat Hippocampal Slice Cultures Open
The hippocampus is essentially involved in learning and memory processes. Its functions are affected by various neuromodulators, including 17β-estradiol, testosterone, and retinoid. Brain-synthesized steroid hormones act as autocrine and p…
View article: Topological Study of Membrane Proteins by Mass Spectrometry
Topological Study of Membrane Proteins by Mass Spectrometry Open
Many static protein structures have been determined by X-ray crystallography and nuclear magnetic resonance (NMR). Protein dynamics has also been studied. The structure and dynamics of proteins provide critical information about their func…
View article: 2P387 Synthesis of brain steroids and localization of P450s in the hippocampal synapses(44. Neuro-biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
2P387 Synthesis of brain steroids and localization of P450s in the hippocampal synapses(44. Neuro-biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006) Open
Estrogens modulaTerranscription known as gcnomic cffects in the braln as in perjpheral organs.But estrugen also elicit rapid noll-genomic effecLs un the synuptic transmission, and spinogenesis in the hippocampus.However in the
View article: 2P386 Synaptic Localization of Estrogen Receptor α in the Rat Hippocampus(44. Neuro-biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
2P386 Synaptic Localization of Estrogen Receptor α in the Rat Hippocampus(44. Neuro-biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006) Open
Estrogens modulaTerranscription known as gcnomic cffects in the braln as in perjpheral organs.But estrugen also elicit rapid noll-genomic effecLs un the synuptic transmission, and spinogenesis in the hippocampus.However in the
View article: Distributions of Steroid 5.ALPHA.-Reductase and 17.ALPHA.-Hydroxylase/C17,20-lyase (P450c17) Immunoreactivities in Rat Gastric Mucosa
Distributions of Steroid 5.ALPHA.-Reductase and 17.ALPHA.-Hydroxylase/C17,20-lyase (P450c17) Immunoreactivities in Rat Gastric Mucosa Open
We examined the immunohistochemical localization of steroid 5α-reductase type 1 and 17α-hydroxylase/C17,20-lyase (P450c17) in rat gastric mucosa. Immunoreactivity of 5α-reductase was localized in the chief cells and the mucous neck cells. …
View article: Adult male rat hippocampus synthesizes estradiol from pregnenolone by cytochromes P45017α and P450 aromatase localized in neurons
Adult male rat hippocampus synthesizes estradiol from pregnenolone by cytochromes P45017α and P450 aromatase localized in neurons Open
In adult mammalian brain, occurrence of the synthesis of estradiol from endogenous cholesterol has been doubted because of the inability to detect dehydroepiandrosterone synthase, P45017α. In adult male rat hippocampal formation, significa…
View article: Cytochrome P450 2D Catalyze Steroid 21-Hydroxylation in the Brain
Cytochrome P450 2D Catalyze Steroid 21-Hydroxylation in the Brain Open
mRNA of cytochrome P450 21-hydroxylase (P450c21) is expressed in the brain, but little is known about the enzymatic properties of P450c21 in the brain. In the present study, we showed, by using various recombinant cytochrome P450 (CYP)2D e…
View article: Corticosterone Enhances Adrenocorticotropin-Induced Calcium Signals in Bovine Adrenocortical Cells
Corticosterone Enhances Adrenocorticotropin-Induced Calcium Signals in Bovine Adrenocortical Cells Open
The rapid effects of steroid hormones on Ca2+ signals have been examined in bovine adrenocortical cells. Among the steroid molecules tested, only corticosterone rapidly stimulated Ca2+ signals upon addition of ACTH, although corticosterone…
View article: The Rate-determining Step in P450 C21-catalyzing Reactions in a Membrane-reconstituted System
The Rate-determining Step in P450 C21-catalyzing Reactions in a Membrane-reconstituted System Open
Adrenal cytochrome P450 C21 in a membrane-reconstituted system catalyzed 21-hydroxylation of 17alpha-hydroxyprogesterone at a rate higher than that for progesterone in the steady state at 37 degrees C. The rate of product formation in the …
View article: Pregnenolone, Pregnenolone Sulfate, and Cytochrome P450 Side-Chain Cleavage Enzyme in the Amphibian Brain and Their Seasonal Changes<sup>1</sup>
Pregnenolone, Pregnenolone Sulfate, and Cytochrome P450 Side-Chain Cleavage Enzyme in the Amphibian Brain and Their Seasonal Changes<sup>1</sup> Open
To clarify whether the amphibian brain synthesizes de novo neurosteroids, we examined pregnenolone, pregnenolone sulfate ester, and cytochrome P450 side-chain cleavage enzyme (cytochrome P450scc), an enzyme converting cholesterol to pregne…
View article: Immunohistochemical Localization of Cytochrome P450 Enzymes in the Rat Brain, Considering the Steroid-Synthesis in the Neurons.
Immunohistochemical Localization of Cytochrome P450 Enzymes in the Rat Brain, Considering the Steroid-Synthesis in the Neurons. Open
To elucidate the steroid-synthesis in the mammalian brain (i. e., neurosteroid), we immunohistochemically studied various kinds of steroidogenic cytochrome P450 enzymes in the rat brain. The primary antibodies used in this study were rabbi…
View article: Molecular Engineering Study on Electron Transfer from NADPH-P450 Reductase to Rat Mitochondrial P450c27 in Yeast Microsomes
Molecular Engineering Study on Electron Transfer from NADPH-P450 Reductase to Rat Mitochondrial P450c27 in Yeast Microsomes Open
We have reported the localization on yeast microsomes for a modified P450c27 (mic-P450c27) that contains the microsomal targeting signal of bovine P450c17 in front of the mature form of rat mitochondrial P450c27 (Sakaki, T., Akiyoshi-Shiba…
View article: 15-lipoxygenase metabolite(s) of arachidonic acid mediates adrenocorticotropin action in bovine adrenal steroidogenesis.
15-lipoxygenase metabolite(s) of arachidonic acid mediates adrenocorticotropin action in bovine adrenal steroidogenesis. Open
The acute activation of adrenal glucocorticoid synthesis by ACTH has long been believed to be mediated by cAMP as the major second messenger, although increases in cellular cAMP concentration have not been observed at low concentrations of…
View article: Intraovarian Immunolocalization of Steroidogenic Enzymes in a Hokkaido Brown Bear, Ursus arctos yesoensis during the Mating Season.
Intraovarian Immunolocalization of Steroidogenic Enzymes in a Hokkaido Brown Bear, Ursus arctos yesoensis during the Mating Season. Open
Immunolocalization for four steroidogenic enzymes was performed on an ovary taken from a Hokkaido brown bear during the mating season. This specimen is considered to be in the follicular phase because of the presence of large follicles. In…
View article: Adrenal P-450scc modulates activity of P-45011 beta in liposomal and mitochondrial membranes. Implication of P-450scc in zone specificity of aldosterone biosynthesis in bovine adrenal.
Adrenal P-450scc modulates activity of P-45011 beta in liposomal and mitochondrial membranes. Implication of P-450scc in zone specificity of aldosterone biosynthesis in bovine adrenal. Open
Bovine adrenal P-45011 beta catalyzes the 11 beta- and 18-hydroxylation of corticosteroids as well as aldosterone synthesis. These activities of P-45011 beta were found to be modulated by another mitochondrial cytochrome P-450 species, P-4…
View article: Immunoreactive cytochrome P-45017 in rat and guineapig gonads, adrenal glands and brain
Immunoreactive cytochrome P-45017 in rat and guineapig gonads, adrenal glands and brain Open
The cytochrome P-450(17 alpha)-hydroxylase, 17----20 lyase (P-450(17 alpha)) is the key enzyme responsible for the biosynthesis of androgens in steroidogenic organs. Its cellular localization has been examined with an immunohistochemical t…
View article: Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex.
Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex. Open
We have isolated cDNA clones of the mRNA for cytochrome P-450 that catalyzes the steroid C-21 hydroxylation (P-450(C21)), which specifically catalyzes 21-hydroxylation of steroids in the microsomes of bovine adrenal cortex by using synthet…
View article: Studies on the interaction of steroid substrates with adrenal microsomal cytochrome P-450 (P-450C21) in liposome membranes.
Studies on the interaction of steroid substrates with adrenal microsomal cytochrome P-450 (P-450C21) in liposome membranes. Open
Cytochrome P-450 (P-450C21), purified from bovine adrenocortical microsomes, was incorporated into the single bilayer liposomes of egg yolk phosphatidylcholine by gel filtration, using a high pressure liquid chromatography system. Interact…
View article: Interaction between cytochrome P-450 (P-450C21) and NADPH-cytochrome P-450 reductase from adrenocortical microsomes in a reconstituted system.
Interaction between cytochrome P-450 (P-450C21) and NADPH-cytochrome P-450 reductase from adrenocortical microsomes in a reconstituted system. Open
The interaction between P-450C21 and NADPH-cytochrome P-450 reductase, both purified from bovine adrenocortical microsomes, has been investigated in a reconstituted system with a nonionic detergent, Emulgen 913, by kinetic analysis and gel…