Alexandra M. Smink
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View article: <i>Bifidobacterium animalis</i> subsp. <i>Lactis</i> BL‐99 Improves Maternal and Fetal Immune Responses and Pregnancy Outcomes in Pregnant Antibiotics‐Treated Mice
<i>Bifidobacterium animalis</i> subsp. <i>Lactis</i> BL‐99 Improves Maternal and Fetal Immune Responses and Pregnancy Outcomes in Pregnant Antibiotics‐Treated Mice Open
The maternal gut microbiome is involved in adapting immune responses to the presence of the semiallogeneic foetus. We have previously shown that antibiotics‐induced gut dysbiosis, alterations in the maternal immune response and decreased f…
View article: Modulating adipose-derived stromal cells' secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation.
Modulating adipose-derived stromal cells' secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation. Open
The secretome of adipose-derived stromal cells (ASC) presents a promising avenue for cell-free therapies due to their rich mixture of bioactive molecules. Different culture conditions can modulate the composition of this mixture, but how t…
View article: ESM‐1 and GBP‐1 are associated with endothelial dysfunction: emerging biomarkers or key players in the pathophysiology of preeclampsia?
ESM‐1 and GBP‐1 are associated with endothelial dysfunction: emerging biomarkers or key players in the pathophysiology of preeclampsia? Open
Preeclampsia is a hypertensive disorder of pregnancy and is one of the most prevalent causes of maternal and fetal morbidity and mortality. The disease is thought to originate from impaired placental development or restricted villous perfu…
View article: Secretome Analysis of Human and Rat Pancreatic Islets Co-Cultured with Adipose-Derived Stromal Cells Reveals a Signature with Enhanced Regenerative Capacities
Secretome Analysis of Human and Rat Pancreatic Islets Co-Cultured with Adipose-Derived Stromal Cells Reveals a Signature with Enhanced Regenerative Capacities Open
Pancreatic islet transplantation (PIT) is a promising treatment for type 1 diabetes (T1D) but faces challenges pre- and post-transplantation. Co-transplantation with mesenchymal stromal cells (MSCs), known for their regenerative properties…
View article: Shaping immunity: the influence of the maternal gut bacteria on fetal immune development
Shaping immunity: the influence of the maternal gut bacteria on fetal immune development Open
View article: Oxygenation and function of endocrine bioartificial pancreatic tissue constructs under flow for preclinical optimization
Oxygenation and function of endocrine bioartificial pancreatic tissue constructs under flow for preclinical optimization Open
Islet transplantation and more recently stem cell-derived islets were shown to successfully re-establish glycemic control in people with type 1 diabetes under immunosuppression. These results were achieved through intraportal infusion whic…
View article: Modulating adipose-derived stromal cells’ secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation
Modulating adipose-derived stromal cells’ secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation Open
The secretome of adipose-derived stromal cells (ASC) presents a promising avenue for cell-free therapies due to their rich mixture of bioactive molecules. Different culture conditions can modulate the composition of this mixture, but how t…
View article: Secretome analysis of human and rat pancreatic islets co-cultured with adipose-derived stromal cells reveals a signature with enhanced regenerative capacities
Secretome analysis of human and rat pancreatic islets co-cultured with adipose-derived stromal cells reveals a signature with enhanced regenerative capacities Open
Pancreatic islet transplantation (PIT), a promising treatment for Type 1 Diabetes (T1D), encounters challenges in the pre- and post-transplantation phases. Co-culturing or co-transplantation of islets with mesenchymal stromal cells (MSC), …
View article: The role of extracellular matrix hydrogels and adipose-derived stromal cells in soft tissue vascularization – A systematic review
The role of extracellular matrix hydrogels and adipose-derived stromal cells in soft tissue vascularization – A systematic review Open
View article: Culturing Conditions Dictate the Composition and Pathways Enrichment of Human and Rat Perirenal Adipose-Derived Stromal Cells’ Secretomes
Culturing Conditions Dictate the Composition and Pathways Enrichment of Human and Rat Perirenal Adipose-Derived Stromal Cells’ Secretomes Open
Understanding the impact of various culturing strategies on the secretome composition of adipose-derived stromal cells (ASC) enhances their therapeutic potential. This study investigated changes in the secretome of perirenal ASC (prASC) un…
View article: Necrostatin‐1 releasing nanoparticles: In vitro and in vivo efficacy for supporting immunoisolated islet transplantation outcomes
Necrostatin‐1 releasing nanoparticles: In vitro and in vivo efficacy for supporting immunoisolated islet transplantation outcomes Open
Immunoisolation of pancreatic islets in alginate microcapsules allows for transplantation in the absence of immunosuppression but graft survival time is still limited. This limited graft survival is caused by a combination of tissue respon…
View article: Microfluidic Generation of Thin‐Shelled Polyethylene Glycol‐Tyramine Microgels for Non‐Invasive Delivery of Immunoprotected β‐Cells
Microfluidic Generation of Thin‐Shelled Polyethylene Glycol‐Tyramine Microgels for Non‐Invasive Delivery of Immunoprotected β‐Cells Open
Transplantation of microencapsulated pancreatic cells is emerging as a promising therapy to replenish β‐cell mass lost from auto‐immune nature of type I diabetes mellitus (T1DM). This strategy intends to use micrometer‐sized microgels to p…
View article: Enhancing longevity of immunoisolated pancreatic islet grafts by modifying both the intracapsular and extracapsular environment
Enhancing longevity of immunoisolated pancreatic islet grafts by modifying both the intracapsular and extracapsular environment Open
View article: Towards standardization of human adipose-derived stromal cells secretomes
Towards standardization of human adipose-derived stromal cells secretomes Open
View article: Mechanisms of Foreign Body Giant Cell Formation in Response to Implantable Biomaterials
Mechanisms of Foreign Body Giant Cell Formation in Response to Implantable Biomaterials Open
Long term function of implantable biomaterials are determined by their integration with the host’s body. Immune reactions against these implants could impair the function and integration of the implants. Some biomaterial-based implants lea…
View article: Extracellular matrix inclusion in immunoisolating alginate-based microcapsules promotes longevity, reduces fibrosis, and supports function of islet allografts in vivo
Extracellular matrix inclusion in immunoisolating alginate-based microcapsules promotes longevity, reduces fibrosis, and supports function of islet allografts in vivo Open
View article: Successful Islet Transplantation Into a Subcutaneous Polycaprolactone Scaffold in Mice and Pigs
Successful Islet Transplantation Into a Subcutaneous Polycaprolactone Scaffold in Mice and Pigs Open
Background. Islet transplantation is a promising treatment for type 1 diabetes. It has the potential to improve glycemic control, particularly in patients suffering from hypoglycemic unawareness and glycemic instability. As most islet graf…
View article: Impact of Exogenous Nitric Oxide Treatment on Vascularization of a Subcutaneous Device for Cell Transplantation
Impact of Exogenous Nitric Oxide Treatment on Vascularization of a Subcutaneous Device for Cell Transplantation Open
Subcutaneous polymer scaffolds have shown potential for creating an optimal transplantation site in cellular replacement therapy, e.g., when transplanting insulin-producing cells to cure type 1 diabetes. Imperative for these scaffolds is a…
View article: Species-dependent impact of immunosuppressive squalene-gusperimus nanoparticles and adipose-derived stem cells on isolated human and rat pancreatic islets
Species-dependent impact of immunosuppressive squalene-gusperimus nanoparticles and adipose-derived stem cells on isolated human and rat pancreatic islets Open
Transplantation of pancreatic islets is a promising approach to controlling glucose levels in type 1 diabetes mellitus (T1DM), but islet survival is still limited. To overcome this, islet co-culture with mesenchymal stromal cells (MSCs) to…
View article: Inclusion of extracellular matrix molecules and necrostatin-1 in the intracapsular environment of alginate-based microcapsules synergistically protects pancreatic β cells against cytokine-induced inflammatory stress
Inclusion of extracellular matrix molecules and necrostatin-1 in the intracapsular environment of alginate-based microcapsules synergistically protects pancreatic β cells against cytokine-induced inflammatory stress Open
Immunoisolation of pancreatic islets in alginate-based microcapsules is a promising approach for grafting of islets in absence of immunosuppression. However, loss and damage to the extracellular matrix (ECM) during islet isolation enhance …
View article: Tethering Cells via Enzymatic Oxidative Crosslinking Enables Mechanotransduction in Non‐Cell‐Adhesive Materials (Adv. Mater. 42/2021)
Tethering Cells via Enzymatic Oxidative Crosslinking Enables Mechanotransduction in Non‐Cell‐Adhesive Materials (Adv. Mater. 42/2021) Open
Cell–Material Tethering Cells need to sense their environment in order to survive and function. In article number 2102660, Tom Kamperman, Jeroen Leijten, and co-workers present an enzymatic crosslinking strategy that can tether non-cell-ad…
View article: Tethering Cells via Enzymatic Oxidative Crosslinking Enables Mechanotransduction in Non‐Cell‐Adhesive Materials
Tethering Cells via Enzymatic Oxidative Crosslinking Enables Mechanotransduction in Non‐Cell‐Adhesive Materials Open
Cell–matrix interactions govern cell behavior and tissue function by facilitating transduction of biomechanical cues. Engineered tissues often incorporate these interactions by employing cell‐adhesive materials. However, using constitutive…
View article: DAMPening COVID-19 Severity by Attenuating Danger Signals
DAMPening COVID-19 Severity by Attenuating Danger Signals Open
COVID-19 might lead to multi-organ failure and, in some cases, to death. The COVID-19 severity is associated with a “cytokine storm.” Danger-associated molecular patterns (DAMPs) are proinflammatory molecules that can activate pattern reco…
View article: In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period
In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period Open
Grafting of microencapsulated pancreatic islets has been proposed as an alternative to exogenous insulin for the treatment of type 1 diabetes mellitus. Microencapsulated islets are protected from direct contact with immune cells and larger…
View article: In vivo vascularization and islet function in a microwell device for pancreatic islet transplantation
In vivo vascularization and islet function in a microwell device for pancreatic islet transplantation Open
Islet encapsulation in membrane-based devices could allow for transplantation of donor islet tissue in the absence of immunosuppression. To achieve long-term survival of islets, the device should allow rapid exchange of essential nutrients…
View article: <i>In vitro</i> determination of the immunosuppressive effect, internalization, and release mechanism of squalene-gusperimus nanoparticles for managing inflammatory responses
<i>In vitro</i> determination of the immunosuppressive effect, internalization, and release mechanism of squalene-gusperimus nanoparticles for managing inflammatory responses Open
Gusperimus is an anti-inflammatory drug that has shown to be effective in managing autoimmunity and preventing graft rejection. This is unstable and easily broken down into cytotoxic components. We encapsulated gusperimus binding it covale…
View article: Toll-like receptor 2-modulating pectin-polymers in alginate-based microcapsules attenuate immune responses and support islet-xenograft survival
Toll-like receptor 2-modulating pectin-polymers in alginate-based microcapsules attenuate immune responses and support islet-xenograft survival Open
View article: Design and characterization of Squalene-Gusperimus nanoparticles for modulation of innate immunity
Design and characterization of Squalene-Gusperimus nanoparticles for modulation of innate immunity Open
View article: Low methyl-esterified pectin protects pancreatic β-cells against diabetes-induced oxidative and inflammatory stress via galectin-3
Low methyl-esterified pectin protects pancreatic β-cells against diabetes-induced oxidative and inflammatory stress via galectin-3 Open
Insufficient intake of dietary fibers in Western societies is considered a major contributing factor in the high incidence rates of diabetes. The dietary fiber pectin has been suggested to be beneficial for management of both Diabetes Type…
View article: In vitro degradation profiles and in vivo biomaterial–tissue interactions of microwell array delivery devices
In vitro degradation profiles and in vivo biomaterial–tissue interactions of microwell array delivery devices Open
To effectively apply microwell array cell delivery devices their biodegradation rate must be tailored towards their intended use and implantation location. Two microwell array devices with distinct degradation profiles, either suitable for…