Alexander Graf
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View article: Radfahren in Zeiten des Klimawandels – macht Hitze das Radfahren (im Alltag) unattraktiver?
Radfahren in Zeiten des Klimawandels – macht Hitze das Radfahren (im Alltag) unattraktiver? Open
Der Beitrag nimmt die durch den Klimawandel bedingte steigende Hitzebelastung zum Anlass und widmet sich vor diesem Hintergrund der Frage, inwiefern hohe Temperaturen einen Einfluss auf die Radnutzung haben. Bisherige Forschung zum Zusamme…
View article: Multimodale Mobilitätsfähigkeit – die Perspektive des Motility-Konzepts
Multimodale Mobilitätsfähigkeit – die Perspektive des Motility-Konzepts Open
Zusammenfassung Mithilfe der Interviews werden zusätzliche Einblicke in die Organisation des Mobilitätsalltags und der Verkehrsmittelnutzung gewonnen, die mit geschlossenen Fragen in Befragungen nicht erhoben werden können. Zum besseren Ve…
View article: NTRC and thioredoxins<i>m</i>1/<i>m</i>2 underpin the light acclimation of plants on proteome and metabolome levels
NTRC and thioredoxins<i>m</i>1/<i>m</i>2 underpin the light acclimation of plants on proteome and metabolome levels Open
During photosynthesis, plants must manage strong fluctuations in light availability on different time scales, leading to long-term acclimation and short-term responses. However, little is known about the regulation and coordination of thes…
View article: A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores
A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores Open
View article: The conditional mitochondrial protein complexome in the Arabidopsis thaliana root and shoot
The conditional mitochondrial protein complexome in the Arabidopsis thaliana root and shoot Open
Protein complexes are important for almost all biological processes. Hence, to fully understand how cells work, it is also necessary to characterize protein complexes and their dynamics in response to various cellular cues. Moreover, the d…
View article: A multifaceted proteomics strategy in combination with an updated proteome provides insights into the mechanism of calcification in coccolithophores
A multifaceted proteomics strategy in combination with an updated proteome provides insights into the mechanism of calcification in coccolithophores Open
Coccolithophores are globally abundant, calcifying microalgae that have profound effects on marine biogeochemical cycles, the climate, and life in the oceans. They are characterized by a cell wall of CaCO3 scales called coccoliths, which m…
View article: A dominant mutation in <i>β-AMYLASE1</i> disrupts nighttime control of starch degradation in Arabidopsis leaves
A dominant mutation in <i>β-AMYLASE1</i> disrupts nighttime control of starch degradation in Arabidopsis leaves Open
Arabidopsis (Arabidopsis thaliana) leaves possess a mechanism that couples the rate of nighttime starch degradation to the anticipated time of dawn, thus preventing premature exhaustion of starch and nighttime starvation. To shed light on …
View article: Topology of the redox network during induction of photosynthesis as revealed by time-resolved proteomics in tobacco
Topology of the redox network during induction of photosynthesis as revealed by time-resolved proteomics in tobacco Open
Plants regulate the distribution of electrons to activate chloroplast metabolism at the onset of light.
View article: Sulfur deficiency-induced genes affect seed protein accumulation and composition under sulfate deprivation
Sulfur deficiency-induced genes affect seed protein accumulation and composition under sulfate deprivation Open
Sulfur deficiency-induced proteins SDI1 and SDI2 play a fundamental role in sulfur homeostasis under sulfate-deprived conditions (−S) by downregulating glucosinolates. Here, we identified that besides glucosinolate regulation under –S, SDI…
View article: Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis
Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis Open
View article: A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts
A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts Open
View article: Separation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes
Separation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes Open
Conventional preparation methods of plant ribosomes fail to resolve non-translating chloroplast or cytoplasmic ribosome subunits from translating fractions. We established preparation of these ribosome complexes from Arabidopsis thaliana l…
View article: FORGETTER2 protein phosphatase and phospholipase D modulate heat stress memory in Arabidopsis
FORGETTER2 protein phosphatase and phospholipase D modulate heat stress memory in Arabidopsis Open
SUMMARY Plants can mitigate environmental stress conditions through acclimation. In the case of fluctuating stress conditions such as high temperatures, maintaining a stress memory enables a more efficient response upon recurring stress. I…
View article: Protein Complex Identification and quantitative complexome by CN-PAGE
Protein Complex Identification and quantitative complexome by CN-PAGE Open
View article: LIKE SEX4 1 Acts as a β-Amylase-Binding Scaffold on Starch Granules during Starch Degradation
LIKE SEX4 1 Acts as a β-Amylase-Binding Scaffold on Starch Granules during Starch Degradation Open
In Arabidopsis (Arabidopsis thaliana) leaves, starch is synthesized during the day and degraded at night to fuel growth and metabolism. Starch is degraded primarily by β-amylases, liberating maltose, but this activity is preceded by…
View article: Relative effects of location relative to the corpus luteum and lactation on the transcriptome of the bovine oviduct epithelium
Relative effects of location relative to the corpus luteum and lactation on the transcriptome of the bovine oviduct epithelium Open
View article: Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana
Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana Open
Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated. In addition, …
View article: Additional file 2: of Relative effects of location relative to the corpus luteum and lactation on the transcriptome of the bovine oviduct epithelium
Additional file 2: of Relative effects of location relative to the corpus luteum and lactation on the transcriptome of the bovine oviduct epithelium Open
Table S2. Exhaustive lists of up- and down-regulated genes in ipsilateral vs. contralateral ampulla in post-partum lactating (L), non-lactating (NL) cows and heifers (H) on Day 3 of the estrous cycle. Lists of associated Gene Ontology (GO)…
View article: Additional file 1: of Relative effects of location relative to the corpus luteum and lactation on the transcriptome of the bovine oviduct epithelium
Additional file 1: of Relative effects of location relative to the corpus luteum and lactation on the transcriptome of the bovine oviduct epithelium Open
Table S1. Exhaustive and top-20 lists of up- and down-regulated genes in post-partum lactating (L) vs. non-lactating (NL) cows, heifers (H) vs. L, and H vs. NL comparisons in the ampulla and isthmus ipsilateral to the corpus luteum on Day …
View article: Additional file 3: of Relative effects of location relative to the corpus luteum and lactation on the transcriptome of the bovine oviduct epithelium
Additional file 3: of Relative effects of location relative to the corpus luteum and lactation on the transcriptome of the bovine oviduct epithelium Open
Table S3. Exhaustive and top-20 lists of up- and down-regulated genes in ipsilateral vs. contralateral isthmus in post-partum lactating (L), non-lactating (NL) cows and heifers (H) on Day 3 of the estrous cycle. Lists of associated Gene On…
View article: Author Index
Author Index Open
View article: <i>AtRsgA</i> from <i>Arabidopsis thaliana</i> is important for maturation of the small subunit of the chloroplast ribosome
<i>AtRsgA</i> from <i>Arabidopsis thaliana</i> is important for maturation of the small subunit of the chloroplast ribosome Open
Summary Plastid ribosomes are very similar in structure and function to the ribosomes of their bacterial ancestors. Since ribosome biogenesis is not thermodynamically favorable under biological conditions it requires the activity of many a…
View article: PROMIS, global analysis of PROtein–metabolite interactions using size separation in Arabidopsis thaliana
PROMIS, global analysis of PROtein–metabolite interactions using size separation in Arabidopsis thaliana Open
View article: Hit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments
Hit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments Open
View article: The Extra-Pathway Interactome of the TCA Cycle: Expected and Unexpected Metabolic Interactions
The Extra-Pathway Interactome of the TCA Cycle: Expected and Unexpected Metabolic Interactions Open
The plant tricarboxylic acid (TCA) cycle provides essential precursors for respiration, amino acid biosynthesis, and general nitrogen metabolism; moreover, it is closely involved in biotic stress responses and cellular redox homeostasis. T…
View article: Interaction of 2',3'-cAMP with Rbp47b plays a role in stress granule formation
Interaction of 2',3'-cAMP with Rbp47b plays a role in stress granule formation Open
29,39-cAMP is an intriguing small molecule that is conserved among different kingdoms. 29,39-cAMP is presumably produced during RNA degradation, with increased cellular levels observed especially under stress conditions. Previously, we obs…
View article: Photoperiodic control of the <i>Arabidopsis</i> proteome reveals a translational coincidence mechanism
Photoperiodic control of the <i>Arabidopsis</i> proteome reveals a translational coincidence mechanism Open
Plants respond to seasonal cues such as the photoperiod, to adapt to current conditions and to prepare for environmental changes in the season to come. To assess photoperiodic responses at the protein level, we quantified the proteome of t…
View article: Temporal Proteomics of Inducible RNAi Lines of Clp Protease Subunits Identifies Putative Protease Substrates
Temporal Proteomics of Inducible RNAi Lines of Clp Protease Subunits Identifies Putative Protease Substrates Open
The Clp protease in the chloroplasts of plant cells is a large complex composed of at least 13 nucleus-encoded subunits and one plastid-encoded subunit, which are arranged in several ring-like structures. The proteolytic P-ring and the str…
View article: <i>AtRsgA</i>from<i>Arabidopsis thaliana</i>controls maturation of the small subunit of the chloroplast ribosome
<i>AtRsgA</i>from<i>Arabidopsis thaliana</i>controls maturation of the small subunit of the chloroplast ribosome Open
Summary Plastid ribosomes are very similar in structure and function to ribosomes of their bacterial ancestors. Since ribosome biogenesis is not thermodynamically favourable at biological conditions, it requires activity of many assembly f…
View article: Effect of lactation on conceptus-maternal interactions at the initiation of implantation in cattle: I. Effects on the conceptus transcriptome and amino acid composition of the uterine luminal fluid†
Effect of lactation on conceptus-maternal interactions at the initiation of implantation in cattle: I. Effects on the conceptus transcriptome and amino acid composition of the uterine luminal fluid† Open
Approximately 65-75 days postpartum (dpp), the estrous cycles of nonlactating (dried off immediately postpartum: n = 12) and lactating (n = 13) Holstein Friesian cows were synchronized and on day 7 a single blastocyst derived from superovu…