Aaron J. Wolfe
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View article: Real-Time Binding Kinetics of Membrane Protein–Protein Interactions in a Membraneless Setting
Real-Time Binding Kinetics of Membrane Protein–Protein Interactions in a Membraneless Setting Open
A ubiquitous problem in protein analytics and medical biotechnology is assessing the interaction of a membrane protein receptor with its cognate protein ligand. This task generally requires transferring the receptor from native membranes o…
View article: Interplay of self-organization of microtubule asters and crosslinking protein condensates
Interplay of self-organization of microtubule asters and crosslinking protein condensates Open
The cytoskeleton is a major focus of physical studies to understand organization inside cells given its primary role in cell motility, cell division, and cell mechanics. Recently, protein condensation has been shown to be another major int…
View article: A generalizable nanopore sensor for highly specific protein detection at single-molecule precision
A generalizable nanopore sensor for highly specific protein detection at single-molecule precision Open
Protein detection has wide-ranging implications in molecular diagnostics. Substantial progress has been made in protein analytics using nanopores and the resistive-pulse technique. Yet, a long-standing challenge is implementing specific in…
View article: Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures
Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures Open
Vimentin intermediate filaments form part of the cytoskeleton of mesenchymal cells, but under pathological conditions often associated with inflammation, vimentin filaments depolymerize as the result of phosphorylation or citrullination, a…
View article: Spatially controlled microtubule nucleation and organization from crosslinker MAP65 condensates
Spatially controlled microtubule nucleation and organization from crosslinker MAP65 condensates Open
Microtubule organization in cells is essential for the internal structure and coordination of events of intracellular transport, mitosis, and cell motility. For many cell types, microtubule organization is dominated by centrosomal nucleati…
View article: A Generalizable Nanopore Sensor for Highly Specific Protein Detection at Single-Molecule Precision
A Generalizable Nanopore Sensor for Highly Specific Protein Detection at Single-Molecule Precision Open
Protein detection and biomarker profiling have wide-ranging implications in many areas of basic research and molecular diagnostics. Substantial progress has been made in protein analytics using nanopores and the resistive-pulse technique. …
View article: Coevolution of the Ess1-CTD axis in polar fungi suggests a role for phase separation in cold tolerance
Coevolution of the Ess1-CTD axis in polar fungi suggests a role for phase separation in cold tolerance Open
Most of the world’s biodiversity lives in cold (−2° to 4°C) and hypersaline environments. To understand how cells adapt to such conditions, we isolated two key components of the transcription machinery from fungal species that live in extr…
View article: P-030: CRIZANLIZUMAB REDUCES ENDOTHELIAL ADHESION OF RED BLOOD CELLS FROM SCD PATIENTS IN STANDARDIZED MICROFLUIDIC PLATFORM: IN VITRO TECHNOLOGY ASSESSMENT
P-030: CRIZANLIZUMAB REDUCES ENDOTHELIAL ADHESION OF RED BLOOD CELLS FROM SCD PATIENTS IN STANDARDIZED MICROFLUIDIC PLATFORM: IN VITRO TECHNOLOGY ASSESSMENT Open
Purpose: Crizanlizumab (ADAKVEO®, Novartis, Switzerland) a monoclonal P-selectin blocking antibody designed to disrupt the blood cells and endothelium interactions in sickle cell disease (SCD) patients, which leads to reduction of vaso-occ…
View article: Convergent Alterations of a Protein Hub Produce Divergent Effects within a Binding Site
Convergent Alterations of a Protein Hub Produce Divergent Effects within a Binding Site Open
Progress in tumor sequencing and cancer databases has created an enormous amount of information that scientists struggle to sift through. While several research groups have created computational methods to analyze these databases, much wor…
View article: Interplay of Affinity and Surface Tethering in Protein Recognition
Interplay of Affinity and Surface Tethering in Protein Recognition Open
Surface-tethered ligand-receptor complexes are key components in biological signaling and adhesion. They also find increasing utility in single-molecule assays and biotechnological applications. Here, we study the real-time binding kinetic…
View article: Disentangling the Recognition Complexity of a Protein Hub Using a Nanopore
Disentangling the Recognition Complexity of a Protein Hub Using a Nanopore Open
WD40 repeat proteins are frequently involved in processing cell signaling and scaffolding large multi-subunit machineries. Despite their significance in physiological and disease-like conditions, their reversible interactions with other pr…
View article: A rare human centenarian variant of SIRT6 enhances genome stability and interaction with Lamin A
A rare human centenarian variant of SIRT6 enhances genome stability and interaction with Lamin A Open
SUMMARY Sirtuin 6 (SIRT6) is a deacylase and mono-ADP ribosyl transferase (mADPr) enzyme involved in multiple cellular pathways implicated in the regulation of aging and metabolism. Targeted sequencing identified a SIRT6 allele containing …
View article: Kinetics of the multitasking high-affinity Win binding site of WDR5 in restricted and unrestricted conditions
Kinetics of the multitasking high-affinity Win binding site of WDR5 in restricted and unrestricted conditions Open
Recent advances in quantitative proteomics show that WD40 proteins play a pivotal role in numerous cellular networks. Yet, they have been fairly unexplored and their physical associations with other proteins are ambiguous. A quantitative u…
View article: CCDC 2043160: Experimental Crystal Structure Determination
CCDC 2043160: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis
Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis Open
Fenretinide is a synthetic retinoid pharmaceutical linked to ceramide build-upin vivo.
View article: High‐Throughput Screening of Protein‐Detergent Complexes Using Fluorescence Polarization Spectroscopy
High‐Throughput Screening of Protein‐Detergent Complexes Using Fluorescence Polarization Spectroscopy Open
This article provides detailed protocols for a high‐throughput fluorescence polarization (FP) spectroscopy approach to disentangle the interactions of membrane proteins with solubilizing detergents. Existing techniques for examining the me…
View article: Kinetics of Membrane Protein–Detergent Interactions Depend on Protein Electrostatics
Kinetics of Membrane Protein–Detergent Interactions Depend on Protein Electrostatics Open
Interactions of a membrane protein with a detergent micelle represent a fundamental process with practical implications in structural and chemical biology. Quantitative assessment of the kinetics of protein-detergent complex (PDC) interact…
View article: Deconvolution of Membrane Protein-Detergent Complex Interactions
Deconvolution of Membrane Protein-Detergent Complex Interactions Open
Membrane proteins are important in many biological functions such as cell-cell recognition, transport, and signaling; yet the study of these proteins is stunted due to their excessive aggregation in aqueous solutions. Detergents have been …
View article: Quantification of Membrane Protein-Detergent Complex Interactions
Quantification of Membrane Protein-Detergent Complex Interactions Open
Although fundamentally significant in structural, chemical, and membrane biology, the interfacial protein-detergent complex (PDC) interactions have been modestly examined because of the complicated behavior of both detergents and membrane …
View article: Interrogating Detergent Desolvation of Nanopore-Forming Proteins by Fluorescence Polarization Spectroscopy
Interrogating Detergent Desolvation of Nanopore-Forming Proteins by Fluorescence Polarization Spectroscopy Open
Understanding how membrane proteins interact with detergents is of fundamental and practical significance in structural and chemical biology as well as in nanobiotechnology. Current methods for inspecting protein-detergent complex (PDC) in…