Aaron K. Neumann
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View article: Plasma Membrane Damage by Environmental Materials Enhances Cell-Cell Fusion and Impairs Immune Functions of Macrophages
Plasma Membrane Damage by Environmental Materials Enhances Cell-Cell Fusion and Impairs Immune Functions of Macrophages Open
Macrophages are the most abundant phagocytes and play an essential role in host defense. Previous studies have shown that many environmental materials can activate macrophages and trigger inflammatory responses. However, whether these expo…
View article: Acoustic pipette and biofunctional elastomeric microparticle system for rapid picomolar-level biomolecule detection in whole blood
Acoustic pipette and biofunctional elastomeric microparticle system for rapid picomolar-level biomolecule detection in whole blood Open
Most biosensing techniques require complex processing steps that generate prolonged workflows and introduce potential points of error. Here, we report an acoustic pipette to purify and label biomarkers in 70 minutes. A key aspect of this t…
View article: Rapid culture free pathogen detection via spectrally resolved fluorescence microscopy
Rapid culture free pathogen detection via spectrally resolved fluorescence microscopy Open
Methods and apparatus for rapid, culture free pathogen detection. The methods utilize optical spectroscopy techniques to identify and/or characterize pathogens in a sample via the detection of unique properties and/or analytes that are spe…
View article: Giardial lipid rafts share virulence factors with secreted vesicles and participate in parasitic infection in mice
Giardial lipid rafts share virulence factors with secreted vesicles and participate in parasitic infection in mice Open
Giardia lamblia , a protozoan parasite, is a major cause of waterborne infection, worldwide. While the trophozoite form of this parasite induces pathological symptoms in the gut, the cyst form transmits the infection. Since Giardia is a no…
View article: Distribution and lateral mobility of DC-SIGN on immature dendritic cells-implications for pathogen uptake
Distribution and lateral mobility of DC-SIGN on immature dendritic cells-implications for pathogen uptake Open
The receptor C-type lectin DC-SIGN (CD209) is expressed by immature dendritic cells, functioning as an antigen capture receptor and cell adhesion molecule. Various microbes, including HIV-1, can exploit binding to DC-SIGN to gain entry to …
View article: Dectin-1-Mediated DC-SIGN Recruitment to Candida albicans Contact Sites
Dectin-1-Mediated DC-SIGN Recruitment to Candida albicans Contact Sites Open
At host–pathogen contact sites with Candida albicans, Dectin-1 activates pro-inflammatory signaling, while DC-SIGN promotes adhesion to the fungal surface. We observed that Dectin-1 and DC-SIGN collaborate to enhance capture/retention of C…
View article: The Formation and Stability of DC-SIGN Microdomains Require its Extracellular Moiety: DC-SIGN Microdomains Require Extracellular Moiety
The Formation and Stability of DC-SIGN Microdomains Require its Extracellular Moiety: DC-SIGN Microdomains Require Extracellular Moiety Open
DC-SIGN (Dendritic cell-specific ICAM-3-grabbing non-integrin) is a Ca2+-dependent transmembrane lectin that binds a large variety of pathogens and facilitates their uptake for subsequent antigen presentation. This receptor is present in c…
View article: Dectin-1 mediated DC-SIGN Recruitment to<i>Candida albicans</i>Contact Sites
Dectin-1 mediated DC-SIGN Recruitment to<i>Candida albicans</i>Contact Sites Open
At host-pathogen contact sites with Candida albicans , Dectin-1 activates pro-inflammatory signaling, while DC-SIGN promotes adhesion to the fungal surface. We observed that Dectin-1 and DC-SIGN collaborate to enhance capture/retention of …
View article: Low Copy Numbers of DC-SIGN in Cell Membrane Microdomains: Implications for Structure and Function: Quantification of DC-SIGN Copy Numbers in Microdomains
Low Copy Numbers of DC-SIGN in Cell Membrane Microdomains: Implications for Structure and Function: Quantification of DC-SIGN Copy Numbers in Microdomains Open
Presently, there are few estimates of the number of molecules occupying membrane domains. Using a total internal reflection fluorescence microscopy (TIRFM) imaging approach, based on comparing the intensities of fluorescently labeled micro…
View article: A Novel Pseudopodial Component of the Dendritic Cell Anti-Fungal Response: The Fungipod
A Novel Pseudopodial Component of the Dendritic Cell Anti-Fungal Response: The Fungipod Open
Fungal pathologies are seen in immunocompromised and healthy humans. C-type lectins expressed on immature dendritic cells (DC) recognize fungi. We report a novel dorsal pseudopodial protrusion, the “fungipod”, formed by DC after contact wi…
View article: Quantitative 3D Tracing of Gene-delivery Viral Vectors in Human Cells and Animal Tissues
Quantitative 3D Tracing of Gene-delivery Viral Vectors in Human Cells and Animal Tissues Open
Trafficking through a variety of cellular structures and organelles is essential for the interaction between gene-delivery vectors (i.e., adeno-associated virus (AAV) and liposomes) and host cells/tissues. Here, we present a method of comp…
View article: DC-SIGN and Influenza Hemagglutinin Dynamics in Plasma Membrane Microdomains Are Markedly Different
DC-SIGN and Influenza Hemagglutinin Dynamics in Plasma Membrane Microdomains Are Markedly Different Open
DC-SIGN, a Ca2+-dependent transmembrane lectin, is found assembled in microdomains on the plasma membranes of dendritic cells. These microdomains bind a large variety of pathogens and facilitate their uptake for subsequent antigen presenta…
View article: Super-Resolution Imaging of C-Type Lectin and Influenza Hemagglutinin Nanodomains on Plasma Membranes Using Blink Microscopy
Super-Resolution Imaging of C-Type Lectin and Influenza Hemagglutinin Nanodomains on Plasma Membranes Using Blink Microscopy Open
Dendritic cells express DC-SIGN, a C-type lectin (CTL) that binds a variety of pathogens and facilitates their uptake for subsequent antigen presentation. DC-SIGN forms remarkably stable microdomains on the plasma membrane. However, inner …
View article: RHOA-mediated mechanical force generation through Dectin-1
RHOA-mediated mechanical force generation through Dectin-1 Open
Dendritic cell-associated C-type lectin 1 (Dectin-1, also known as CLEC7A) is an innate immune pattern recognition receptor that recognizes β-glucan on the Candida albicans cell wall. Recognition of β-glucan by immune cells leads to phagoc…
View article: Dectin-1 Molecular Aggregation and Signaling is Sensitive to β-Glucan Structure and Glucan Exposure on <i>Candida albicans</i> Cell Walls
Dectin-1 Molecular Aggregation and Signaling is Sensitive to β-Glucan Structure and Glucan Exposure on <i>Candida albicans</i> Cell Walls Open
Dectin-1A is a C-type Lectin innate immunoreceptor that recognizes β-(1,3;1,6)-glucan, a structural component of Candida species cell walls. The higher order structure of β-glucans ranges from random coil to insoluble fiber due to varying …
View article: RHOA mediated mechanical force generation through Dectin-1
RHOA mediated mechanical force generation through Dectin-1 Open
Dectin-1 is an innate immune pattern recognition receptor which recognizes β-glucan on the Candida albicans ( C. albicans ) cell wall. Recognition of β-glucan by immune cells leads to phagocytosis, oxidative burst, cytokine and chemokine p…
View article: Mannan Molecular Substructures Control Nanoscale Glucan Exposure in Candida
Mannan Molecular Substructures Control Nanoscale Glucan Exposure in Candida Open
Cell wall mannans of Candida albicans mask β-(1,3)-glucan from recognition by Dectin-1, contributing to innate immune evasion. Glucan exposures are predominantly single receptor-ligand interaction sites of nanoscale dimensions. Candida spe…
View article: A computational model for regulation of nanoscale glucan exposure in Candida albicans
A computational model for regulation of nanoscale glucan exposure in Candida albicans Open
Candida albicans is a virulent human opportunistic pathogen. It evades innate immune surveillance by masking an immunogenic cell wall polysaccharide, β-glucan, from recognition by the immunoreceptor Dectin-1. Glucan unmasking by the antifu…