Aaron P. Mitchell
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View article: Hgt17-Adr1 Relationship in Candida albicans Citrate Utilization
Hgt17-Adr1 Relationship in Candida albicans Citrate Utilization Open
The fungal pathogen Candida albicans can infect diverse tissues, a reflection of its broad metabolic repertoire. The transcription factor Adr1 is required for utilization of several citric acid cycle intermediates that are found in tissue.…
View article: Characterization of<i>ORF19.7608</i>(<i>PPP1</i>), a Biofilm-induced Gene of<i>Candida albicans</i>
Characterization of<i>ORF19.7608</i>(<i>PPP1</i>), a Biofilm-induced Gene of<i>Candida albicans</i> Open
The opportunistic human pathogen Candida albicans is an important cause of nosocomial infections, in large part because of its propensity to form biofilms on indwelling medical devices such as catheters. The formation of these biofilms is …
View article: Control of citrate utilization by <i>Candida albicans</i> Adr1
Control of citrate utilization by <i>Candida albicans</i> Adr1 Open
Candida albicans , a fungal commensal and pathogen, occupies diverse niches in the human host. Its broad metabolic repertoire is critical for its survival. The model yeast Saccharomyces cerevisiae provides a starting point for analysis of …
View article: Roles of P-body factors in Candida albicans filamentation and stress response
Roles of P-body factors in Candida albicans filamentation and stress response Open
Hyphal growth is strongly associated with virulence in the human fungal pathogen Candida albicans . While hyphal transcriptional networks have been the subject of intense study, relatively little is known about post-transcriptional regulat…
View article: Strain-limited biofilm regulation through the Brg1-Rme1 circuit in <i>Candida albicans</i>
Strain-limited biofilm regulation through the Brg1-Rme1 circuit in <i>Candida albicans</i> Open
Prominent virulence traits of Candida albicans include its ability to produce filamentous hyphal cells and grow as a biofilm. These traits are under control of numerous transcription factors (TFs), including Brg1 and Rme1. In the reference…
View article: Strain variation in <i>Candida albicans</i> glycolytic gene regulation
Strain variation in <i>Candida albicans</i> glycolytic gene regulation Open
Central carbon metabolism is vital for the proliferation of Candida albicans , a fungus that is prominent as a commensal and pathogen. Glycolytic genes are activated by overlapping activities of the transcription factors Tye7 and Gal4, as …
View article: A Brg1-Rme1 circuit in <i>Candida albicans</i> hyphal gene regulation
A Brg1-Rme1 circuit in <i>Candida albicans</i> hyphal gene regulation Open
Major Candida albicans virulence traits include its ability to make hyphae, to produce a biofilm, and to damage host cells. These traits depend upon expression of hypha-associated genes. A gene expression comparison among clinical isolates…
View article: Roles of P-body factors in <i>Candida albicans</i> filamentation and stress response
Roles of P-body factors in <i>Candida albicans</i> filamentation and stress response Open
Hyphal growth is strongly associated with virulence in the human fungal pathogen Candida albicans . While hyphal transcriptional networks have been the subject of intense study, relatively little is known about post-transcriptional regulat…
View article: Strain variation in the <i>Candida albicans</i> iron limitation response
Strain variation in the <i>Candida albicans</i> iron limitation response Open
Iron acquisition is critical for pathogens to proliferate during invasive infection, and the human fungal pathogen Candida albicans is no exception. The iron regulatory network, established in reference strain SC5314 and derivatives, inclu…
View article: Systematic analysis of the <i>Candida albicans</i> kinome reveals environmentally contingent protein kinase-mediated regulation of filamentation and biofilm formation <i>in vitro</i> and <i>in vivo</i>
Systematic analysis of the <i>Candida albicans</i> kinome reveals environmentally contingent protein kinase-mediated regulation of filamentation and biofilm formation <i>in vitro</i> and <i>in vivo</i> Open
Protein kinases are critical regulatory proteins in both prokaryotes and eukaryotes. Accordingly, protein kinases represent a common drug target for a wide range of human diseases. Therefore, understanding protein kinase function in human …
View article: Biofilm-associated metabolism via ERG251 in Candida albicans
Biofilm-associated metabolism via ERG251 in Candida albicans Open
Biofilm formation by the fungal pathogen Candida albicans is the basis for its ability to infect medical devices. The metabolic gene ERG251 has been identified as a target of biofilm transcriptional regulator Efg1, and here we report that …
View article: Regulatory features of <i>Candida albicans</i> hemin-induced filamentation
Regulatory features of <i>Candida albicans</i> hemin-induced filamentation Open
Candida albicans is a prominent fungal pathogen that can infect the bloodstream and deep tissues. One key pathogenicity trait is the ability to transition between yeast and hyphal growth. Hyphae are critical for the formation of biofilms, …
View article: MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues
MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues Open
Super‐resolution optical imaging tools are crucial in microbiology to understand the complex structures and behavior of microorganisms such as bacteria, fungi, and viruses. However, the capabilities of these tools, particularly when it com…
View article: Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection
Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection Open
Fungal invasion of the oral epithelium is central to the pathogenesis of oropharyngeal candidiasis (OPC). Candida albicans invades the oral epithelium by receptor-induced endocytosis but this process is incompletely understood. We found th…
View article: A New Expansion Microscopy Method Optimized for Microbiology
A New Expansion Microscopy Method Optimized for Microbiology Open
Journal Article A New Expansion Microscopy Method Optimized for Microbiology Get access Zhangyu Cheng, Zhangyu Cheng Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA Search for other works by thi…
View article: Glucose-enhanced oxidative stress resistance—A protective anticipatory response that enhances the fitness of Candida albicans during systemic infection
Glucose-enhanced oxidative stress resistance—A protective anticipatory response that enhances the fitness of Candida albicans during systemic infection Open
Most microbes have developed responses that protect them against stresses relevant to their niches. Some that inhabit reasonably predictable environments have evolved anticipatory responses that protect against impending stresses that are …
View article: Strain variation in gene expression impact of hyphal cyclin Hgc1 in <i>Candida albicans</i>
Strain variation in gene expression impact of hyphal cyclin Hgc1 in <i>Candida albicans</i> Open
Formation of hyphae is a key virulence trait of the fungal pathogen Candida albicans. Hypha morphogenesis depends upon the cyclin Hgc1, which acts together with cyclin-dependent protein kinase Cdc28 to phosphorylate effectors that drive po…
View article: Functional Dichotomy for a Hyphal Repressor in Candida albicans
Functional Dichotomy for a Hyphal Repressor in Candida albicans Open
Hypha formation is a central virulence trait of the fungal pathogen Candida albicans . Control of hypha formation has been studied in detail in the type strain but not in other diverse C. albicans clinical isolates.
View article: MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues
MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues Open
Super-resolution optical imaging tools are crucial in microbiology to understand the complex structures and behavior of microorganisms such as bacteria, fungi, and viruses. However, the capabilities of these tools, particularly when it com…
View article: Correction: Targeted Genetic Changes in <i>Candida albicans</i> Using Transient CRISPR‐Cas9 Expression
Correction: Targeted Genetic Changes in <i>Candida albicans</i> Using Transient CRISPR‐Cas9 Expression Open
Current Protocols is issuing corrections for the following protocol article. Huang, M. Y., Cravener, M. V., & Mitchell, A. P. (2021). Targeted genetic changes in Candida albicans using transient CRISPR-Cas9 expression. Current Protocols, 1…
View article: <i>Candida albicans</i>stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection
<i>Candida albicans</i>stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection Open
Summary Fungal invasion of the oral epithelium is central to the pathogenesis of oropharyngeal candidiasis (OPC). Candida albicans invades the oral epithelium by receptor-induced endocytosis but this process is incompletely understood. We …
View article: Hgc1 Independence of Biofilm Hyphae in Candida albicans
Hgc1 Independence of Biofilm Hyphae in Candida albicans Open
The HGC1 gene encodes a cyclin that is required for virulence of the fungal pathogen Candida albicans . It is required to produce the elongated hyphal filaments of free-living planktonic cells that are associated with virulence.
View article: Reinforcement amid genetic diversity in the Candida albicans biofilm regulatory network
Reinforcement amid genetic diversity in the Candida albicans biofilm regulatory network Open
Biofilms of the fungal pathogen Candida albicans include abundant long filaments called hyphae. These cells express hypha-associated genes, which specify diverse virulence functions including surface adhesins that ensure biofilm integrity.…
View article: Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation
Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation Open
Candida species commonly form mixed-species biofilms with other Candida species and bacteria. In the established biofilm state, vesicle cargo delivers public goods to support the mature community.
View article: Nature of β-1,3-Glucan-Exposing Features on Candida albicans Cell Wall and Their Modulation
Nature of β-1,3-Glucan-Exposing Features on Candida albicans Cell Wall and Their Modulation Open
Microbes that coexist with humans have evolved ways of avoiding or evading our immunological defenses. These include the masking by these microbes of their “pathogen-associated molecular patterns” (PAMPs), which are recognized as “foreign”…
View article: The future of fungi: threats and opportunities
The future of fungi: threats and opportunities Open
The fungal kingdom represents an extraordinary diversity of organisms with profound impacts across animal, plant, and ecosystem health. Fungi simultaneously support life, by forming beneficial symbioses with plants and producing life-savin…