Naeem Rashid
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View article: ELECTROCONDUCTIVITY PROFILES ON SPRAY DRYING: FEASIBILITY STUDY ON REDUCING FOULING DEPOSIT ON BOROSILICATE DRYING CHAMBER SURFACE VIA ALTERNATING REPULSIVE CHARGES
ELECTROCONDUCTIVITY PROFILES ON SPRAY DRYING: FEASIBILITY STUDY ON REDUCING FOULING DEPOSIT ON BOROSILICATE DRYING CHAMBER SURFACE VIA ALTERNATING REPULSIVE CHARGES Open
Spray drying is a conventional technique for converting liquids into powders, involving preparation, homogenization, atomization, dispersion, and drying. However, electrostatic charges on the drying chamber walls and powder particles often…
View article: Investigation on the Effect of Alginate Concentration and Flow Rate on Production of Nanoparticle Loaded with Naringenin Using Electrospray Method
Investigation on the Effect of Alginate Concentration and Flow Rate on Production of Nanoparticle Loaded with Naringenin Using Electrospray Method Open
View article: A Bifunctional Phosphoglucomutase/Phosphomannomutase from Thermococcus kodakarensis: Biophysical Analysis and Cryo-EM Structure
A Bifunctional Phosphoglucomutase/Phosphomannomutase from Thermococcus kodakarensis: Biophysical Analysis and Cryo-EM Structure Open
Phosphoglucomutase (EC 5.4.2.2., PGM), a key enzyme of glycogenolysis and glycogenesis, catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate, whereas phosphomannomutase (EC 5.4.2.8., PMM) transfers the phosphate gro…
View article: Overproduction of Tk-Subtilisin and Elucidation of Its Structural Thermostability Based on Molecular Dynamics Studies
Overproduction of Tk-Subtilisin and Elucidation of Its Structural Thermostability Based on Molecular Dynamics Studies Open
Background: Subtilisin from Thermococcus kodakaraensis (Tk-subtilisin) is an ideal candidate for many industrial and research processes due to its unique features. Methods: In this study, the enzyme is overproduced in E. coli using four di…
View article: Biophysical Characterization of a Novel Phosphopentomutase from the Hyperthermophilic Archaeon Thermococcus kodakarensis
Biophysical Characterization of a Novel Phosphopentomutase from the Hyperthermophilic Archaeon Thermococcus kodakarensis Open
Phosphopentomutases catalyze the isomerization of ribose 1-phosphate and ribose 5-phosphate. Thermococcus kodakarensis, a hyperthermophilic archaeon, harbors a novel enzyme (PPMTk) that exhibits high homology with phosphohexomutases but ha…
View article: 3D modeling of thermostable xylanase from Thermotoga naphthophila a member of GH10 family: characterization studies of recombinant xylanase
3D modeling of thermostable xylanase from Thermotoga naphthophila a member of GH10 family: characterization studies of recombinant xylanase Open
The current study was planned keeping in view the significance, industrial impact and import of xylanase to Pakistan. In this study, a thermostable recombinant xylanase from Thermotoga naphthophila was produced and characterized. The PCR p…
View article: Comparison of Mulligan Mobilization with Movement and Progressive Strengthening Exercises in Patient with Lateral Epicondylitis
Comparison of Mulligan Mobilization with Movement and Progressive Strengthening Exercises in Patient with Lateral Epicondylitis Open
Background: Lateral epicondylitis, commonly known as tennis elbow, is the most prevalent cause of elbow pain. Both Mulligan Mobilization with Movement (MWM) and Progressive Resistive Exercises (PRE) have shown effectiveness in treating lat…
View article: RISK FACTORS ASSOCIATED WITH BIRTH ASPHYXIA
RISK FACTORS ASSOCIATED WITH BIRTH ASPHYXIA Open
This study aimed to identify factors contributing to prenatal asphyxia in full-term neonates born at Hayatabad Medical Complex in Peshawar. The research was conducted over one year (January 2022 to January 2023) at the Pediatrics Departmen…
View article: Ethylenediaminetetraacetic acid enhances structural stability and thermotolerance of recombinant cyclomaltodextrinase from Geobacillus thermopakistaniensis at higher temperatures.
Ethylenediaminetetraacetic acid enhances structural stability and thermotolerance of recombinant cyclomaltodextrinase from Geobacillus thermopakistaniensis at higher temperatures. Open
Ethylenediaminetetraacetic acid (EDTA), a chelating agent, has shown the ability to enhance the thermostability of cyclomaltodextrinase from Geobacillus thermopakistaniensis (CDaseGt). There was a 5-fold and 3-fold enhancement i…
View article: Development of an immunodiagnostic assay for the detection of Sugarcane mosaic virus
Development of an immunodiagnostic assay for the detection of Sugarcane mosaic virus Open
The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Figure S1. SnapGene map of the SCMVCpaj405-pET28 (a+) expression construct for E. coli. Figure S2. Transf…
View article: Development of an immunodiagnostic assay for the detection of Sugarcane mosaic virus
Development of an immunodiagnostic assay for the detection of Sugarcane mosaic virus Open
Sugarcane mosaic virus (SCMV) has devastating yield losses in sugarcane cultivars around the World, including Pakistan. The disease losses can be minimized, and specific control measures can be employed if the disease could be diagnosed at…
View article: Temperature dependent autocleavage and applications of recombinant L-asparaginase from Thermococcus kodakarensis for acrylamide mitigation
Temperature dependent autocleavage and applications of recombinant L-asparaginase from Thermococcus kodakarensis for acrylamide mitigation Open
View article: Structural and Functional Analyses of a Highly Thermoactive Atp-Dependent Hexokinase from Pyrobaculum Calidifontis
Structural and Functional Analyses of a Highly Thermoactive Atp-Dependent Hexokinase from Pyrobaculum Calidifontis Open
View article: Investigating the role of carbohydrate-binding module 34 in cyclomaltodextrinase from Geobacillus thermopakistaniensis: structural and functional analyses
Investigating the role of carbohydrate-binding module 34 in cyclomaltodextrinase from Geobacillus thermopakistaniensis: structural and functional analyses Open
View article: A novel RdRp-based colorimetric RT-LAMP assay for rapid and sensitive detection of SARS-CoV-2 in clinical and sewage samples from Pakistan
A novel RdRp-based colorimetric RT-LAMP assay for rapid and sensitive detection of SARS-CoV-2 in clinical and sewage samples from Pakistan Open
View article: Gene Cloning and Characterization of Pcal_0222, α-Amylase from Pyrobaculum calidifontis
Gene Cloning and Characterization of Pcal_0222, α-Amylase from Pyrobaculum calidifontis Open
The current research trial was conducted to evaluate the effects of a non-antibiotic growth promoter on growth performance and histomorphology in broiler chickens.One hundred and twenty day-old broiler chicks were randomly distributed into…
View article: Cyclodextrin-preferring glycoside hydrolases: properties and applications
Cyclodextrin-preferring glycoside hydrolases: properties and applications Open
Cyclodextrin-hydrolyzing enzymes are widespread in bacteria and archaea where they play their roles in carbohydrates metabolism. They were previously characterized as cyclodextrinases, neopullulanases and maltogenic amylases. In the Carboh…
View article: Purification and Characterization of a Thermostable Pyruvate Ferredoxin Oxidoreductase/Pyruvate Decarboxylase from Thermococcus kodakaraensis
Purification and Characterization of a Thermostable Pyruvate Ferredoxin Oxidoreductase/Pyruvate Decarboxylase from Thermococcus kodakaraensis Open
View article: TK-PUL, a pullulan hydrolase type III from <i>Thermococcus kodakarensis</i>, a potential candidate for simultaneous liquefaction and saccharification of starch
TK-PUL, a pullulan hydrolase type III from <i>Thermococcus kodakarensis</i>, a potential candidate for simultaneous liquefaction and saccharification of starch Open
TK-PUL, a novel thermo-acidophilic pullulanase from Thermococcus kodakarensis and a unique member of glycoside hydrolase family GH13 was successfully produced in Escherichia coli grown by fed batch culture in a fermenter and partially puri…
View article: Brewer’s rice - A potential substrate for cosmeceutical bio-ingredient production by solid state fermentation using Aspergillus oryzae
Brewer’s rice - A potential substrate for cosmeceutical bio-ingredient production by solid state fermentation using Aspergillus oryzae Open
View article: Molecular Identification and Characterization of Lactic Acid producing Bacterial Strains Isolated from Raw and Traditionally Processed Foods of Punjab, Pakistan
Molecular Identification and Characterization of Lactic Acid producing Bacterial Strains Isolated from Raw and Traditionally Processed Foods of Punjab, Pakistan Open
View article: Escherichia coli Signal Peptidases Cleave the Signal Sequence of TK0522, a Carbohydrate Esterase from Hyperthermophilic Archaeon Thermococcus kodakarensis
Escherichia coli Signal Peptidases Cleave the Signal Sequence of TK0522, a Carbohydrate Esterase from Hyperthermophilic Archaeon Thermococcus kodakarensis Open
TK0522 gene, encoding a carbohydrate esterase, from Thermococcus kodakarensis was cloned with its native signal sequence and expressed in Escherichia coli.Heterologous gene expression resulted in production of recombinant protein in the cy…
View article: Effect of fertilizers and chiseling techniques on optimizing growth of cotton (Gossypium hirsutum L.)
Effect of fertilizers and chiseling techniques on optimizing growth of cotton (Gossypium hirsutum L.) Open
Cotton contributes significantly to the production of oilseeds and textile fiber globally, and it is an important component of the socioeconomic and political environment. This study aims to evaluate the effects of commercial sulfuric acid…
View article: Valorization of waste foods using pullulan hydrolase from Thermococcus kodakarensis
Valorization of waste foods using pullulan hydrolase from Thermococcus kodakarensis Open
The starchy foods including staled or leftover bread, insect infested or damaged cereal grains and bakery wastes are usually discarded, which is a threat to public health due to extensive fungal growth. In the present study we have utilize…
View article: Gene Expression in Escherichia coli and Purification of Recombinant Type II Pullulanase from a Hyperthermophilic Archaeon, Pyrobaculum calidifontis
Gene Expression in Escherichia coli and Purification of Recombinant Type II Pullulanase from a Hyperthermophilic Archaeon, Pyrobaculum calidifontis Open
View article: A Phosphofructokinase Homolog from Pyrobaculum calidifontis Displays Kinase Activity towards Pyrimidine Nucleosides and Ribose 1-Phosphate
A Phosphofructokinase Homolog from Pyrobaculum calidifontis Displays Kinase Activity towards Pyrimidine Nucleosides and Ribose 1-Phosphate Open
The discovery of the pentose bisphosphate pathway in Thermococcus kodakarensis has clarified how this archaeon can degrade nucleosides. Homologs of the enzymes of this pathway are present in many members of the Thermococcales, suggesting t…
View article: Gene cloning and characterization of recombinant L-Asparaginase from Bacillus subtilis strain R5
Gene cloning and characterization of recombinant L-Asparaginase from Bacillus subtilis strain R5 Open
View article: Refolding of Misfolded Inclusion Bodies of Recombinant α-Amylase: Characterization of Cobalt Activated Thermostable α-Amylase from Geobacillus SBS-4S
Refolding of Misfolded Inclusion Bodies of Recombinant α-Amylase: Characterization of Cobalt Activated Thermostable α-Amylase from Geobacillus SBS-4S Open
View article: Complete signal peptide of Tk1884, an α-amylase from Thermococcus kodakarensis, is not necessary for extracellular secretion of the enzyme by Escherichia coli
Complete signal peptide of Tk1884, an α-amylase from Thermococcus kodakarensis, is not necessary for extracellular secretion of the enzyme by Escherichia coli Open
In order to elucidate if Escherichia coli secretion system recognizes the N-terminally truncated signal sequence of an archaeal α-amylase from Thermococcus kodakarensis (Tk1884) and secretes the recombinant protein to the extracellular med…
View article: Characterization of Recombinant Thermostable Phytase from Thermotoga naphthophila: A Step for the Fulfilment of Domestic Requirement of Phytase in Pakistan
Characterization of Recombinant Thermostable Phytase from Thermotoga naphthophila: A Step for the Fulfilment of Domestic Requirement of Phytase in Pakistan Open