Abigail L. Mackey
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View article: Spatially distinct ECM-producing fibroblasts and myonuclei orchestrate early adaptation to mechanical loading in the human muscle-tendon unit
Spatially distinct ECM-producing fibroblasts and myonuclei orchestrate early adaptation to mechanical loading in the human muscle-tendon unit Open
We provide the first high-resolution, in vivo single-nucleus map of the acute human muscle-tendon response to mechanical loading. Within 4 hours of resistance exercise, spatially distinct tendon fibroblasts and myonuclei activate coordinat…
View article: Spatially distinct ECM-producing fibroblasts and myonuclei orchestrate early adaptation to mechanical loading in the human muscle-tendon unit
Spatially distinct ECM-producing fibroblasts and myonuclei orchestrate early adaptation to mechanical loading in the human muscle-tendon unit Open
Mechanical loading drives structural and functional improvements in muscle and tendon, protecting against injury at their interface – the myotendinous junction (MTJ) – and within the tendon matrix. However, the early cellular and molecular…
View article: Morphological differences in myofibre size and shape: A comparative study of the soleus, gastrocnemius, triceps brachii and vastus lateralis in humans and mice
Morphological differences in myofibre size and shape: A comparative study of the soleus, gastrocnemius, triceps brachii and vastus lateralis in humans and mice Open
Certain skeletal muscles are specialized for their functional roles, yet direct comparisons of cellular morphology of distinct muscles beyond fibre type distribution are limited. This study investigated myofibre morphology in predominantly…
View article: Serum C‐Terminal Agrin Fragment With Acute and Long‐Term Exercise and Angiotensin II Type I Receptor Blockade
Serum C‐Terminal Agrin Fragment With Acute and Long‐Term Exercise and Angiotensin II Type I Receptor Blockade Open
Background Sarcopenia represents a major clinical and societal challenge facing rapidly aging populations. Accessible and specific biomarkers represent valuable tools, both in diagnosis and assessing the efficacy of therapeutic interventio…
View article: Heavy Resistance Exercise Training in Older Men: A Responder and Inter-individual Variability Analysis
Heavy Resistance Exercise Training in Older Men: A Responder and Inter-individual Variability Analysis Open
Background Heavy resistance exercise training (HReT) effectively increases muscle mass and strength in groups of older individuals. However, the extent of inter-individual variability in response to HReT, and the possible existence of non-…
View article: Insights into human muscle biology from human primary skeletal muscle cell culture
Insights into human muscle biology from human primary skeletal muscle cell culture Open
This review arises from the symposium held in honour of Prof Jenny Morgan at UCL in 2024 and the authors would like to acknowledge the outstanding contribution that Prof Morgan has made to the field of translational muscle cell biology. Pr…
View article: The repair capacity spectrum of human skeletal muscle injury from sports to surgical trauma settings
The repair capacity spectrum of human skeletal muscle injury from sports to surgical trauma settings Open
Skeletal muscle injury and repair have been a major research focus for more than a century. Muscle injuries are defined by their cause and anatomical location and lie on a spectrum in terms of repair outcomes. From contraction‐induced necr…
View article: Cardiorespiratory Fitness, Physical Performance and Metabolic Syndrome in Adult Survivors of Paediatric Haematopoietic Stem Cell Transplantation
Cardiorespiratory Fitness, Physical Performance and Metabolic Syndrome in Adult Survivors of Paediatric Haematopoietic Stem Cell Transplantation Open
Background We examined cardiorespiratory fitness and physical performance in long‐term survivors of paediatric haematopoietic stem cell transplantation (HSCT) and explored how these are associated with the presence of metabolic syndrome (M…
View article: Muscle fibroblasts and stem cells stimulate motor neurons in an age and exercise‐dependent manner
Muscle fibroblasts and stem cells stimulate motor neurons in an age and exercise‐dependent manner Open
Exercise preserves neuromuscular function in aging through unknown mechanisms. Skeletal muscle fibroblasts (FIB) and stem cells (MuSC) are abundant in skeletal muscle and reside close to neuromuscular junctions, but their relative roles in…
View article: Muscle Fibroblasts and Stem Cells Stimulate Motor Neurons in An Age and Exercise-Dependent Manner
Muscle Fibroblasts and Stem Cells Stimulate Motor Neurons in An Age and Exercise-Dependent Manner Open
Exercise preserves neuromuscular function in ageing through unknown mechanisms. Skeletal muscle fibroblasts (FIB) and stem cells (MuSC) are abundant in skeletal muscle and reside close to neuromuscular junctions, but their relative roles i…
View article: Insulin‐like growth factor‐1 infusion in preterm piglets does not affect growth parameters of skeletal muscle or tendon tissue
Insulin‐like growth factor‐1 infusion in preterm piglets does not affect growth parameters of skeletal muscle or tendon tissue Open
Prematurity has physical consequences, such as lower birth weight, decreased muscle mass and increased risk of adult‐onset metabolic disease. Insulin‐like growth factor 1 (IGF‐1) has therapeutic potential to improve the growth and quality …
View article: Bone remodeling in survivors of pediatric hematopoietic stem cell transplantation: Impact of heavy resistance training
Bone remodeling in survivors of pediatric hematopoietic stem cell transplantation: Impact of heavy resistance training Open
Background Early‐onset osteoporosis is a frequent late effect after pediatric hematopoietic stem cell transplantation (HSCT). It remains unknown if physical training can improve bone formation in these patients, as the transplantation proc…
View article: Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres
Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres Open
The myotendinous junction (MTJ) is a weak link in the musculoskeletal system. Here, we isolated the tips of single myofibres from healthy human hamstring muscles for confocal microscopy (n=6) and RNAscope in situ hybridization (n=6) to gai…
View article: Return to Sport, Reinjury Rate, and Tissue Changes after Muscle Strain Injury: A Narrative Review
Return to Sport, Reinjury Rate, and Tissue Changes after Muscle Strain Injury: A Narrative Review Open
A major challenge in sports medicine is to facilitate the fastest possible recovery from injury without increasing the risk of subsequent reruptures, and thus effective rehabilitation programs should balance between these two factors. The …
View article: Marked irregular myofiber shape is a hallmark of human skeletal muscle ageing and is reversed by heavy resistance training
Marked irregular myofiber shape is a hallmark of human skeletal muscle ageing and is reversed by heavy resistance training Open
Background Age‐related loss of strength is disproportionally greater than the loss of mass, suggesting maladaptations in the neuro‐myo‐tendinous system. Myofibers are often misshaped in aged and diseased muscle, but systematic analyses of …
View article: The effects of resistance training on denervated myofibers, senescent cells, and associated protein markers in middle-aged adults
The effects of resistance training on denervated myofibers, senescent cells, and associated protein markers in middle-aged adults Open
Denervated myofibers and senescent cells are hallmarks of skeletal muscle aging. However, sparse research has examined how resistance training affects these outcomes. We investigated the effects of unilateral leg extensor resistance traini…
View article: Fusion of myofibre branches is a physiological feature of healthy human skeletal muscle regeneration
Fusion of myofibre branches is a physiological feature of healthy human skeletal muscle regeneration Open
Background The occurrence of hyperplasia, through myofibre splitting, remains a widely debated phenomenon. Structural alterations and fibre typing of skeletal muscle fibres, as seen during regeneration and in certain muscle diseases, can b…
View article: Mechanisms of mechanical overload-induced skeletal muscle hypertrophy: current understanding and future directions
Mechanisms of mechanical overload-induced skeletal muscle hypertrophy: current understanding and future directions Open
Mechanisms underlying mechanical overload-induced skeletal muscle hypertrophy have been extensively researched since the landmark report by Morpurgo (1897) of “work-induced hypertrophy” in dogs that were treadmill trained. Much of the prec…
View article: Marked irregular myofiber shape is a hallmark of human skeletal muscle aging and is reversed by heavy resistance training
Marked irregular myofiber shape is a hallmark of human skeletal muscle aging and is reversed by heavy resistance training Open
Background Age-related loss of strength is disproportionally greater than the loss of mass, suggesting maladaptations in the neuro-myo-tendinous system. Myofibers are often misshaped in aged and diseased muscle, but systematic analyses of …
View article: Fusion of myofibre branches is a physiological feature of healthy human skeletal muscle regeneration
Fusion of myofibre branches is a physiological feature of healthy human skeletal muscle regeneration Open
Background : The occurrence of hyperplasia, through myofibre splitting, remains a widely debated phenomenon. Structural alterations and fibre typing of skeletal muscle fibres, as seen during regeneration and in certain muscle diseases, can…
View article: Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing
Distinct myofibre domains of the human myotendinous junction revealed by single-nucleus RNA sequencing Open
The myotendinous junction (MTJ) is a specialized domain of the multinucleated myofibre that is faced with the challenge of maintaining robust cell–matrix contact with the tendon under high mechanical stress and strain. Here, we profiled 24…
View article: Distinct myofibre domains of the human myotendinous junction revealed by single nucleus RNA-seq
Distinct myofibre domains of the human myotendinous junction revealed by single nucleus RNA-seq Open
The myotendinous junction (MTJ) is a specialized domain of the multinucleated myofibre, faced with the challenge of maintaining robust cell-matrix contact with the tendon under high mechanical stress and strain. Here, we profiled 24,161 nu…
View article: PCM1 labeling reveals myonuclear and nuclear dynamics in skeletal muscle across species
PCM1 labeling reveals myonuclear and nuclear dynamics in skeletal muscle across species Open
Myonuclei transcriptionally regulate muscle fibers during homeostasis and adaptation to exercise. Their subcellular location and quantity are important when characterizing phenotypes of myopathies, the effect of treatments, and understandi…
View article: Larger interface area at the human myotendinous junction in type 1 compared with type 2 muscle fibers
Larger interface area at the human myotendinous junction in type 1 compared with type 2 muscle fibers Open
The myotendinous junction (MTJ) is structurally specialized to transmit force. The highly folded muscle membrane at the MTJ increases the contact area between muscle and tendon and potentially the load tolerance of the MTJ. Muscles with a …
View article: Aging is associated with an altered macrophage response during human skeletal muscle regeneration
Aging is associated with an altered macrophage response during human skeletal muscle regeneration Open
Skeletal muscle injury in aged rodents is characterized by an asynchronous infiltration of pro- and anti-inflammatory macrophage waves, leading to improper and incomplete regeneration. It is unclear whether this aberration also occurs in a…
View article: An Estimate of Plasma Volume Changes Following Moderate-High Intensity Running and Cycling Exercise and Adrenaline Infusion
An Estimate of Plasma Volume Changes Following Moderate-High Intensity Running and Cycling Exercise and Adrenaline Infusion Open
Introduction: Plasma volume (PV) changes in response to physical activity, possibly as a consequence of adrenergic activation. We estimated changes in PV in response to common exercise modalities; cycling and running as well as adrenaline …
View article: Intramuscular connective tissue content and mechanical properties: Influence of aging and physical activity in mice
Intramuscular connective tissue content and mechanical properties: Influence of aging and physical activity in mice Open
Aging is accompanied by morphological and mechanical changes to the intramuscular connective tissue (IMCT) of skeletal muscles, but whether physical exercise can influence these changes is debated. We investigated the effects of aging and …
View article: AB0984 CHANGES IN PLASMA VOLUME WHEN MEASURING BIOCHEMICAL MARKERS OF JOINT TISSUE TURNOVER IN RELATION TO ACUTE PHYSICAL ACTIVITY
AB0984 CHANGES IN PLASMA VOLUME WHEN MEASURING BIOCHEMICAL MARKERS OF JOINT TISSUE TURNOVER IN RELATION TO ACUTE PHYSICAL ACTIVITY Open
View article: Human skeletal muscle acetylcholine receptor gene expression in elderly males performing heavy resistance exercise
Human skeletal muscle acetylcholine receptor gene expression in elderly males performing heavy resistance exercise Open
Muscle fiber denervation is a major contributor to the decline in muscle mass and function during aging. Heavy resistance exercise is an effective tool for increasing muscle mass and strength, but whether it can rescue denervated muscle fi…
View article: A RANDOMIZED, CROSS-OVER STUDY TO INVESTIGATE THE EFFECT OF WEIGHT-BEARING VS NON-WEIGHT-BEARING EXERCISE AND CARDIOVASCULAR STRESS ON TYPE II COLLAGEN TURNOVER IN PATIENTS WITH KNEE OSTEOARTHRITIS - SERUM BIOMARKER DATA FROM THE EFEX-OA-02 STUDY
A RANDOMIZED, CROSS-OVER STUDY TO INVESTIGATE THE EFFECT OF WEIGHT-BEARING VS NON-WEIGHT-BEARING EXERCISE AND CARDIOVASCULAR STRESS ON TYPE II COLLAGEN TURNOVER IN PATIENTS WITH KNEE OSTEOARTHRITIS - SERUM BIOMARKER DATA FROM THE EFEX-OA-02 STUDY Open