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View article: A conformational transition of the D′D3 domain primes von Willebrand factor for multimerization
A conformational transition of the D′D3 domain primes von Willebrand factor for multimerization Open
Von Willebrand factor (VWF) is a multimeric plasma glycoprotein that is critically involved in hemostasis. Biosynthesis of long VWF concatemers in the endoplasmic reticulum and the trans-Golgi is still not fully understood. We use the sing…
View article: Author Correction: Vascular surveillance by haptotactic blood platelets in inflammation and infection
Author Correction: Vascular surveillance by haptotactic blood platelets in inflammation and infection Open
The original version of this Article contained an error in the "Introduction", as reference was missing.The original version read incorrectly "[…] since the abrogation of lamellipodia formation does not
View article: A Conformational Transition of Von Willebrand Factor’s D’D3 Domain Primes It For Multimerization
A Conformational Transition of Von Willebrand Factor’s D’D3 Domain Primes It For Multimerization Open
Von Willebrand factor (VWF) is a multimeric plasma glycoprotein that is critically involved in hemostasis. Biosynthesis of long VWF concatemers in the endoplasmic reticulum and the (trans-)Golgi is still not fully understood. We use the si…
View article: Designed Anchoring Geometries Determine Lifetimes of Biotin-Streptavidin Bonds under Constant Load and Enable Ultra-Stable Coupling
Designed Anchoring Geometries Determine Lifetimes of Biotin-Streptavidin Bonds under Constant Load and Enable Ultra-Stable Coupling Open
The small molecule biotin and the homotetrameric protein streptavidin (SA) form a stable and robust complex that plays a pivotal role in many biotechnological and medical applications. In particular, the biotin-streptavidin linkage is freq…
View article: Multiplexed protein force spectroscopy reveals equilibrium protein folding dynamics and the low-force response of von Willebrand factor
Multiplexed protein force spectroscopy reveals equilibrium protein folding dynamics and the low-force response of von Willebrand factor Open
Significance The physiological function of proteins is often critically regulated by mechanical forces acting on them. Single-molecule manipulation techniques such as atomic force microscopy or optical tweezers have enabled unprecedented i…
View article: Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging
Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging Open
The formation of hemostatic plugs at sites of vascular injury crucially involves the multimeric glycoprotein von Willebrand factor (VWF). VWF multimers are linear chains of N-terminally linked dimers. The latter are formed from monomers vi…
View article: Single-molecule mechanics and regulatory conformational transitions of the force-sensing protein von Willebrand factor
Single-molecule mechanics and regulatory conformational transitions of the force-sensing protein von Willebrand factor Open
The formation of hemostatic plugs at sites of vascular injury represents a first essential step in the blood coagulation cascade. This process crucially relies on the large, linear multimeric glycoprotein von Willebrand factor (VWF) and it…
View article: Modular, ultra-stable, and highly parallel protein force spectroscopy in magnetic tweezers using peptide linkers
Modular, ultra-stable, and highly parallel protein force spectroscopy in magnetic tweezers using peptide linkers Open
Single-molecule force spectroscopy has provided unprecedented insights into protein folding, force-regulation, and function. Here, we present a modular magnetic tweezers force spectroscopy approach that uses elastin-like polypeptide linker…
View article: Force sensing by the vascular protein von Willebrand factor is tuned by a strong intermonomer interaction
Force sensing by the vascular protein von Willebrand factor is tuned by a strong intermonomer interaction Open
Significance Excessive blood loss at a site of vascular injury is prevented by recruitment of platelets to the injured vessel wall and the formation of a platelet plug. Under elevated shear flow conditions, these processes are critically m…