Adam E. Cohen
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View article: Distribution of Eigenvalues of the Kohn Laplacian on Sphere Quotients
Distribution of Eigenvalues of the Kohn Laplacian on Sphere Quotients Open
We prove a Weyl-type theorem for the Kohn Laplacian on sphere quotients as CR manifolds. We show that we can determine the fundamental group from the spectrum of the Kohn Laplacian in dimension three. Furthermore, we prove Sobolev estimate…
View article: Electrophysiology in small compartments
Electrophysiology in small compartments Open
Voltage-gated ion channels are found in many membrane-enclosed structures, including synaptic vesicles, endosomes, mitochondria, chloroplasts, viruses and bacteria. In small compartments, assumptions underlying Hodgkin-Huxley-type models m…
View article: Optical single-channel recording via diffusional confinement in membrane tethers
Optical single-channel recording via diffusional confinement in membrane tethers Open
Single-channel electrophysiology probes ion channel gating, but how can one probe membrane transport when the single-unit current is undetectable? We pulled membrane tethers from live cells to isolate individual transmembrane proteins. The…
View article: Optogenetic control of Nodal signaling patterns
Optogenetic control of Nodal signaling patterns Open
A crucial step in early embryogenesis is the establishment of spatial patterns of signaling activity. Tools to perturb morphogen signals with high resolution in space and time can help reveal how embryonic cells decode these signals to mak…
View article: Mechanism of giant magnetic field effect in fluorescence of mScarlet3, a red fluorescent protein
Mechanism of giant magnetic field effect in fluorescence of mScarlet3, a red fluorescent protein Open
Several fluorescent proteins, when expressed in E. coli , are sensitive to weak magnetic fields 1 . We found that mScarlet3 fluorescence in E. coli reversibly decreased by 21% in the presence of a 60 mT magnetic field, the largest magnetic…
View article: Luminos: open-source software for bidirectional microscopy
Luminos: open-source software for bidirectional microscopy Open
Bidirectional microscopy (BDM) combines simultaneous targeted optical perturbation and imaging of biophysical or biochemical signals (e.g. membrane voltage, Ca 2+ , or signaling molecules). A core challenge in BDM is precise spatial and te…
View article: Swimming motions evoke Ca<sup>2+</sup>events in vascular endothelial cells of larval zebrafish via mechanical activation of Piezo1
Swimming motions evoke Ca<sup>2+</sup>events in vascular endothelial cells of larval zebrafish via mechanical activation of Piezo1 Open
Summary Calcium signaling in blood vessels regulates their growth 1,2 , immune response 3 , and vascular tone 4 . Vascular endothelial cells are known to be mechanosensitive 5–7 , and it has been assumed that this mechanosensation mediates…
View article: Dendritic excitations govern back-propagation via a spike-rate accelerometer
Dendritic excitations govern back-propagation via a spike-rate accelerometer Open
Dendrites on neurons support electrical excitations, but the computational significance of these events is not well understood. We developed molecular, optical, and computational tools for all-optical electrophysiology in dendrites. We map…
View article: Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators
Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators Open
Microbial rhodopsin–derived genetically encoded voltage indicators (GEVIs) are powerful tools for mapping bioelectrical dynamics in cell culture and in live animals. Förster resonance energy transfer (FRET)–opsin GEVIs use voltage-dependen…
View article: Ketamine induces plasticity in a norepinephrine-astroglial circuit to promote behavioral perseverance
Ketamine induces plasticity in a norepinephrine-astroglial circuit to promote behavioral perseverance Open
Transient exposure to ketamine can trigger lasting changes in behavior and mood. We found that brief ketamine exposure causes long-term suppression of futility-induced passivity in larval zebrafish, reversing the "giving-up" response that …
View article: All-optical mapping of Ca2+ transport and homeostasis in dendrites
All-optical mapping of Ca2+ transport and homeostasis in dendrites Open
Calcium mediates many important signals in dendrites. However, the basic transport properties of calcium in dendrites have been difficult to measure: how far and how fast does a local influx of calcium propagate? We developed an all-optica…
View article: Tools for intersectional optical and chemical tagging on cell surfaces
Tools for intersectional optical and chemical tagging on cell surfaces Open
We present versatile tools for intersectional optical and chemical tagging of live cells. Photocaged tetrazines serve as “photo-click” adapters between recognition groups on the cell surface and diverse chemical payloads. We describe two n…
View article: Mapping uterine calcium dynamics during the ovulatory cycle in live mice
Mapping uterine calcium dynamics during the ovulatory cycle in live mice Open
Uterine contraction patterns vary during the ovulatory cycle and throughout pregnancy, but prior measurements have produced limited and conflicting information on these patterns. We combined a virally delivered genetically encoded calcium …
View article: Optical constraints on two-photon voltage imaging
Optical constraints on two-photon voltage imaging Open
Due to the stringent photon-count requirements of voltage imaging and the modest voltage sensitivity of existing reporters, 2P voltage imaging in vivo faces a stringent tradeoff between shot noise and tissue photodamage. 2P imaging of hund…
View article: All-optical mapping of Ca<sup>2+</sup>transport and homeostasis in dendrites
All-optical mapping of Ca<sup>2+</sup>transport and homeostasis in dendrites Open
Calcium mediates many important signals in dendrites. However, the basic transport properties of calcium in dendrites have been difficult to measure: how far and how fast does a local influx of calcium propagate? We developed an all-optica…
View article: Optogenetic control of Nodal signaling patterns
Optogenetic control of Nodal signaling patterns Open
A crucial step in early embryogenesis is the establishment of spatial patterns of signaling activity. Tools to perturb morphogen signals with high resolution in space and time can help reveal how embryonic cells decode these signals to mak…
View article: Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators
Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators Open
Microbial rhodopsin-derived genetically encoded voltage indicators (GEVIs) are powerful tools for mapping bioelectrical dynamics in cell culture and in live animals. Förster resonance energy transfer (FRET)-opsin GEVIs use voltage-dependen…
View article: Ether lipids influence cancer cell fate by modulating iron uptake
Ether lipids influence cancer cell fate by modulating iron uptake Open
Cancer cell fate has been widely ascribed to mutational changes within protein-coding genes associated with tumor suppressors and oncogenes. In contrast, the mechanisms through which the biophysical properties of membrane lipids influence …
View article: Mapping uterine calcium dynamics during the ovulatory cycle in live mice
Mapping uterine calcium dynamics during the ovulatory cycle in live mice Open
Uterine contraction patterns vary during the ovulatory cycle and throughout pregnancy but prior measurements have produced limited and conflicting information on these patterns. We combined a virally delivered genetically encoded calcium r…
View article: Solaris: a panel of bright and sensitive hybrid voltage indicators for imaging membrane potential in cultured neurons
Solaris: a panel of bright and sensitive hybrid voltage indicators for imaging membrane potential in cultured neurons Open
Dynamic changes in the membrane potential underlie neuronal activities. Fluorescent voltage indicators allow optical recording of electrical signaling across a neuronal population with cellular precision and at millisecond-level temporal r…
View article: Spiking at the edge: Excitability at interfaces in reaction–diffusion systems
Spiking at the edge: Excitability at interfaces in reaction–diffusion systems Open
Excitable media, ranging from bioelectric tissues and chemical oscillators to forest fires and competing populations, are nonlinear, spatially extended systems capable of spiking. Most investigations of excitable media consider situations …
View article: Code and data for "Spiking at the edge: Excitability at interfaces in reaction-diffusion systems"
Code and data for "Spiking at the edge: Excitability at interfaces in reaction-diffusion systems" Open
This .zip file contains code and data to support the paper "Spiking at the edge: Excitability at interfaces in reaction-diffusion systems" https://doi.org/10.1073/pnas.2307996120). See Readme.txt for technical details.
View article: Optical constraints on two-photon voltage imaging
Optical constraints on two-photon voltage imaging Open
Significance Genetically encoded voltage indicators (GEVIs) are a valuable tool for studying neural circuits in vivo , but the relative merits and limitations of one-photon (1P) vs. two-photon (2P) voltage imaging are not well characterize…
View article: A phenotypic screening platform for chronic pain therapeutics using all-optical electrophysiology
A phenotypic screening platform for chronic pain therapeutics using all-optical electrophysiology Open
Chronic pain associated with osteoarthritis (OA) remains an intractable problem with few effective treatment options. New approaches are needed to model the disease biology and to drive discovery of therapeutics. We present an in vitro mod…
View article: Optical segmentation-based compressed readout of neuronal voltage dynamics
Optical segmentation-based compressed readout of neuronal voltage dynamics Open
Functional imaging of biological dynamics generally begins with acquiring time-series images, followed by quantifying spatially averaged intensity traces for the regions of interest (ROIs). The conventional pipeline discards a substantial …
View article: Current Status and Future Strategies for Advancing Functional Circuit Mapping<i>In Vivo</i>
Current Status and Future Strategies for Advancing Functional Circuit Mapping<i>In Vivo</i> Open
The human brain represents one of the most complex biological systems, containing billions of neurons interconnected through trillions of synapses. Inherent to the brain is a biochemical complexity involving ions, signaling molecules, and …