Adam Lange
YOU?
Author Swipe
View article: Atomistic Mechanism of Calcium-Mediated Inward Rectification of the MthK Potassium Channel by Solid-State NMR and MD Simulations
Atomistic Mechanism of Calcium-Mediated Inward Rectification of the MthK Potassium Channel by Solid-State NMR and MD Simulations Open
Inward rectification is a fundamental but poorly understood phenomenon in potassium channel physiology. Despite its physiological importance, the exact mechanism has remained elusive. In this work, we uncover a previously unrecognized calc…
View article: Death, taxes, and rhomboids: Understanding the ubiquitous roles of the rhomboid protein superfamily
Death, taxes, and rhomboids: Understanding the ubiquitous roles of the rhomboid protein superfamily Open
The rhomboid superfamily is the largest family of membrane proteins, containing over 122,000 members (both active and inactive proteases) across nearly all domains of life. The high number of members, as well as the conserved roles underta…
View article: Atomistic Mechanism of Calcium-Mediated Inward Rectification of the MthK Potassium Channel by Solid-State NMR and MD Simulations
Atomistic Mechanism of Calcium-Mediated Inward Rectification of the MthK Potassium Channel by Solid-State NMR and MD Simulations Open
Inward rectification is a fundamental but poorly understood phenomenon in potassium channel physiology. Despite its physiological importance, the exact mechanism has remained elusive. In this work, we uncover a previously unrecognized calc…
View article: Carbazole‐Based Thin Microporous Polymer Films for Photocatalytic Hydrogen Evolution
Carbazole‐Based Thin Microporous Polymer Films for Photocatalytic Hydrogen Evolution Open
Photocatalytic hydrogen evolution is a direct pathway to store solar energy in chemicals. Conjugated microporous polymers (CMPs) are porous organic photocatalysts, that are typically applied in powder form in heterogenous catalytic reactio…
View article: Structural Transition from Closed to Open for the Influenza A M2 Proton Channel as Observed by Proton-Detected Solid-State NMR
Structural Transition from Closed to Open for the Influenza A M2 Proton Channel as Observed by Proton-Detected Solid-State NMR Open
The influenza A M2 protein is an acid-activated proton channel and an established pharmaceutical target for antiflu drugs. Here, we studied the conductance domain of the tetrameric M2 channel (construct 18-60) using proton-detected solid-s…
View article: Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG Open
Chemical shift assignments of large membrane proteins by solid-state NMR experiments are challenging. Recent advancements in sensitivity-enhanced pulse sequences, have made it feasible to acquire 1 H-detected 4D spectra of these challengin…
View article: Lipid-polymer nanoparticles to probe the native-like environment of intramembrane rhomboid protease GlpG and its activity
Lipid-polymer nanoparticles to probe the native-like environment of intramembrane rhomboid protease GlpG and its activity Open
Polymers can facilitate detergent-free extraction of membrane proteins into nanodiscs (e.g., SMALPs, DIBMALPs), incorporating both integral membrane proteins as well as co-extracted native membrane lipids. Lipid-only SMALPs and DIBMALPs ha…
View article: Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG Open
Chemical shift assignments of large membrane proteins by solid-state NMR experiments are challenging. Recent advancements in sensitivity-enhanced pulse sequences, have made it feasible to acquire 1H-detected 4D spectra of these challenging…
View article: An anionic two dimensional covalent organic framework from tetratopic borate centres pillared by lithium ions
An anionic two dimensional covalent organic framework from tetratopic borate centres pillared by lithium ions Open
Non-covalent interactions play an important role for the framework formation of two-dimensional covalent organic frameworks. Until now, π–π interactions and hydrogen bonding are the main reported forces facilitating the stacking of framewo…
View article: Homonuclear Simplified Preservation of Equivalent Pathways Spectroscopy
Homonuclear Simplified Preservation of Equivalent Pathways Spectroscopy Open
Recently developed homonuclear transverse mixing optimal control pulses (hTROP) revealed an elegant way to enhance the detected signal in multidimensional magic-angle spinning (MAS) nuclear magnetic resonance experiments. Inspired by their…
View article: Homonuclear Simplified Preservation of Equivalent Pathways Spectroscopy
Homonuclear Simplified Preservation of Equivalent Pathways Spectroscopy Open
Recently developed homonuclear Transverse mixing Optimal control Pulses (hTROP) revealed an elegant way to enhance the detected signal in multi-dimensional magic-angle spinning (MAS) NMR experiments. Inspired by their work, we present two …
View article: In Situ Spectroscopic Detection of Large‐Scale Reorientations of Transmembrane Helices During Influenza A M2 Channel Opening**
In Situ Spectroscopic Detection of Large‐Scale Reorientations of Transmembrane Helices During Influenza A M2 Channel Opening** Open
Viroporins are small ion channels in membranes of enveloped viruses that play key roles during viral life cycles. To use viroporins as drug targets against viral infection requires in‐depth mechanistic understanding and, with that, methods…
View article: The opening dynamics of the lateral gate regulates the activity of rhomboid proteases
The opening dynamics of the lateral gate regulates the activity of rhomboid proteases Open
Rhomboid proteases hydrolyze substrate helices within the lipid bilayer to release soluble domains from the membrane. Here, we investigate the mechanism of activity regulation for this unique but wide-spread protein family. In the model rh…
View article: In-situ spectroscopic detection of large-scale reorientations of transmembrane α-helices during viroporin channel opening
In-situ spectroscopic detection of large-scale reorientations of transmembrane α-helices during viroporin channel opening Open
Viroporins are small ion channels in membranes of enveloped viruses that play key roles during viral life cycles. To use viroporins as drug targets against viral infection requires in-depth mechanistic understanding and, with that, methods…
View article: Accurate Determination of Motional Amplitudes in Biomolecules by Solid-State NMR
Accurate Determination of Motional Amplitudes in Biomolecules by Solid-State NMR Open
Protein dynamics are an intrinsically important factor when considering a protein's biological function. Understanding these motions is often limited through the use of static structure determination methods, namely, X-ray crystallography …
View article: Photoactivation of a Mechanosensitive Channel
Photoactivation of a Mechanosensitive Channel Open
Optogenetics in the conventional sense, i.e. the use of engineered proteins that gain their light sensitivity from naturally abundant chromophores, represents an exciting means to trigger and control biological activity by light. As an alt…
View article: Direct Detection of Bound Ammonium Ions in the Selectivity Filter of Ion Channels by Solid-State NMR
Direct Detection of Bound Ammonium Ions in the Selectivity Filter of Ion Channels by Solid-State NMR Open
The flow of ions across cell membranes facilitated by ion channels is an important function for all living cells. Despite the huge amount of structural data provided by crystallography, elucidating the exact interactions between the select…
View article: Structural Plasticity of the Selectivity Filter in Cation Channels
Structural Plasticity of the Selectivity Filter in Cation Channels Open
Ion channels allow for the passage of ions across biological membranes, which is essential for the functioning of a cell. In pore loop channels the selectivity filter (SF) is a conserved sequence that forms a constriction with multiple ion…
View article: Structural plasticity of the selectivity filter in a nonselective ion channel
Structural plasticity of the selectivity filter in a nonselective ion channel Open
The sodium potassium ion channel (NaK) is a nonselective ion channel that conducts both sodium and potassium across the cellular membrane. A new crystallographic structure of NaK reveals conformational differences in the residues that make…
View article: A combination of solid-state NMR and MD simulations reveals the binding mode of a rhomboid protease inhibitor
A combination of solid-state NMR and MD simulations reveals the binding mode of a rhomboid protease inhibitor Open
Proton-detected solid-state NMR in combination with molecular docking and molecular dynamics (MD) simulations allow the study of rhomboid protease inhibition under native-like conditions.
View article: Spinal Column Architecture of the Flexible SPP1 Bacteriophage Tail Tube
Spinal Column Architecture of the Flexible SPP1 Bacteriophage Tail Tube Open
Phage therapy has recently regained attention at combating multidrug-resistant bacteria. In 2019, tailed bacteriophages of the Siphoviridae family were engineered to successfully treat a disseminated bacterial infection after all other dru…
View article: Protein resonance assignment by BSH‐CP‐based 3D solid‐state NMR experiments: A practical guide
Protein resonance assignment by BSH‐CP‐based 3D solid‐state NMR experiments: A practical guide Open
Solid‐state NMR (ssNMR) spectroscopy has evolved into a powerful method to obtain structural information and to study the dynamics of proteins at atomic resolution and under physiological conditions. The method is especially well suited to…
View article: Dynamic interactions of CbiN and CbiM trigger activity of a cobalt energy-coupling-factor transporter
Dynamic interactions of CbiN and CbiM trigger activity of a cobalt energy-coupling-factor transporter Open
Energy-coupling factor (ECF) transporters for uptake of vitamins and transition-metal ions into prokaryotic cells share a common architecture consisting of a substrate-specific integral membrane protein (S), a transmembrane coupling protei…
View article: The conduction pathway of potassium channels is water free under physiological conditions
The conduction pathway of potassium channels is water free under physiological conditions Open
Detection of bound water in a potassium channel by solid-state NMR supports the “direct” knock-on ion permeation mechanism.
View article: Protein−Protein Interfaces Probed by Methyl Labeling and Proton‐Detected Solid‐State NMR Spectroscopy
Protein−Protein Interfaces Probed by Methyl Labeling and Proton‐Detected Solid‐State NMR Spectroscopy Open
Proton detection and fast magic‐angle spinning have advanced biological solid‐state NMR, allowing for the backbone assignment of complex protein assemblies with high sensitivity and resolution. However, so far no method has been proposed t…