Adam Z. Higgins
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View article: Saliency and trial context in embodied cues of spatial attention: Pointing to context
Saliency and trial context in embodied cues of spatial attention: Pointing to context Open
Recent work suggests that the pointing hand on an outstretched arm is possibly a more powerful cue than the gaze-cue, suggesting these embodied social cues are not equal. The aim of this study is to investigate differences between gaze- an…
View article: Screening for cryoprotective agent toxicity and toxicity reduction in mixtures at subambient temperatures
Screening for cryoprotective agent toxicity and toxicity reduction in mixtures at subambient temperatures Open
Organ transplantation faces major challenges in preserving and transporting organs due to the limitations of existing cold storage methods. Cryopreservation offers a promising alternative for extending preservation time, but it remains a c…
View article: High-Throughput Evaluation of Cryoprotective Agents for Mixture Effects That Reduce Toxicity
High-Throughput Evaluation of Cryoprotective Agents for Mixture Effects That Reduce Toxicity Open
Vitrification is a promising approach for cryopreserving complex biological structures such as organs. However, to prevent ice formation, high concentrations of cell-permeable cryoprotective agents (CPAs) are required, which can be highly …
View article: High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents
High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents Open
Cryoprotective agent (CPA) toxicity is the most limiting factor impeding cryopreservation of critically needed tissues and organs for transplantation and medical research. This limitation is in part due to the challenge of rapidly screenin…
View article: Cryopreserving the intact intervertebral disc without compromising viability
Cryopreserving the intact intervertebral disc without compromising viability Open
Background Tissue cryopreservation requires saturation of the structure with cryoprotectants (CPAs) that are also toxic to cells within a short timeframe unless frozen. The race between CPA delivery and cell death is the main barrier to re…
View article: High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents
High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents Open
Vitrification is the most promising method for cryopreservation of complex structures such as organs and tissue constructs. However, this method requires multimolar concentrations of cell-permeant cryoprotective agents (CPAs), which can be…
View article: Osmotic response during kidney perfusion with cryoprotectant in isotonic or hypotonic vehicle solution
Osmotic response during kidney perfusion with cryoprotectant in isotonic or hypotonic vehicle solution Open
Organ cryopreservation would revolutionize transplantation by overcoming the shelf-life limitations of conventional organ storage. To prepare an organ for cryopreservation, it is first perfused with cryoprotectants (CPAs). These chemicals …
View article: Accelerating cryoprotectant delivery using vacuum infiltration
Accelerating cryoprotectant delivery using vacuum infiltration Open
The ability to cryopreserve bone marrow within the vertebral body (VB) would offer significant clinical and research benefits. However, cryopreservation of large structures, such as VBs, is challenging due to mass transport limitations tha…
View article: The effects of cryoprotectant, temperature and length of in vitro culture in the permeability of in vitro produced bovine embryos: Towards an in silico designed vitrification/warming protocol
The effects of cryoprotectant, temperature and length of in vitro culture in the permeability of in vitro produced bovine embryos: Towards an in silico designed vitrification/warming protocol Open
The oleaginous microalga Lobosphaera incisa (Trebouxiophyceae, Chlorophyta) contains arachidonic acid (ARA, 20:4 n − 6) in all membrane glycerolipids and in the storage lipid triacylglycerol. The optimal growth temperature of the wild-type…
View article: Multiple cryoprotectant toxicity model for vitrification solution optimization
Multiple cryoprotectant toxicity model for vitrification solution optimization Open
View article: Impact of equilibration duration combined with temperature on the outcome of bovine oocyte vitrification
Impact of equilibration duration combined with temperature on the outcome of bovine oocyte vitrification Open
The cryopreservation of mammalian oocytes and embryos has become an integral part of assisted reproduction in both humans and veterinary species. However, the methods used to cryopreserve bovine oocytes still have significant shortcomings.…
View article: The Role of Aquaporin 7 in the Movement of Water and Cryoprotectants in Bovine In Vitro Matured Oocytes
The Role of Aquaporin 7 in the Movement of Water and Cryoprotectants in Bovine In Vitro Matured Oocytes Open
Aquaglyceroporins are known as channel proteins, and are able to transport water and small neutral solutes. In this study, we evaluate the effect of exposure of in vitro matured bovine oocytes to hyperosmotic solutions containing ethylene …
View article: Microfluidic photoreactor to treat neonatal jaundice
Microfluidic photoreactor to treat neonatal jaundice Open
While in most cases, jaundice can be effectively treated using phototherapy, severe cases require exchange transfusion, a relatively risky procedure in which the neonate's bilirubin-rich blood is replaced with donor blood. Here, we examine…
View article: General tissue mass transfer model for cryopreservation applications
General tissue mass transfer model for cryopreservation applications Open
View article: Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches Open
View article: Rapid quantification of multi-cryoprotectant toxicity using an automated liquid handling method
Rapid quantification of multi-cryoprotectant toxicity using an automated liquid handling method Open
View article: Using Studios as a Strategy to Respond to Increasing Enrollment
Using Studios as a Strategy to Respond to Increasing Enrollment Open
Using Studios as a Strategy to Respond to Increasing EnrollmentRecently chemical engineering programs in many universities have shown significant growthrelated to student interest in energy production, sustainability, and innovation chemic…
View article: Mathematical Modeling of Protectant Transport in Tissues
Mathematical Modeling of Protectant Transport in Tissues Open
View article: Implications of variability in cell membrane permeability for design of methods to remove glycerol from frozen-thawed erythrocytes
Implications of variability in cell membrane permeability for design of methods to remove glycerol from frozen-thawed erythrocytes Open
View article: Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer
Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer Open
Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and …
View article: A toxicity cost function approach to optimal CPA equilibration in tissues
A toxicity cost function approach to optimal CPA equilibration in tissues Open
View article: Determination of methemoglobin and hemoglobin levels in small volume samples
Determination of methemoglobin and hemoglobin levels in small volume samples Open
Background/aims Hemoglobin-based oxygen carriers (HBOCs) have been previously studied as resuscitation fluids. Due to HBOCs specific molecular conformation, hemoglobin (Hb) and methemoglobin (MetHb) determination is not always possible wit…
View article: Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells
Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells Open
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vi…