Adele Williamson
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View article: Crystal structure of ATP-dependent DNA ligase from <i>Rhizobium</i> phage vB_RleM_P10VF
Crystal structure of ATP-dependent DNA ligase from <i>Rhizobium</i> phage vB_RleM_P10VF Open
DNA ligases are foundational molecular-biological tools used for cloning and sequencing workflows, and are essential replicative enzymes for all cellular life forms as well as many viruses and bacteriophage. There is considerable interest …
View article: Influence of the ATP-dependent DNA ligase, Lig E, on <i>Neisseria gonorrhoeae</i> microcolony and biofilm formation
Influence of the ATP-dependent DNA ligase, Lig E, on <i>Neisseria gonorrhoeae</i> microcolony and biofilm formation Open
Background Neisseria gonorrhoeae , the causative agent of the sexually transmitted infection, gonorrhoea, is known to form biofilms rich in extracellular DNA on human cervical cells. Biofilm formation is conducive to increased antimicrobia…
View article: Nucleic acid joining enzymes: biological functions and synthetic applications beyond DNA
Nucleic acid joining enzymes: biological functions and synthetic applications beyond DNA Open
DNA-joining by ligase and polymerase enzymes has provided the foundational tools for generating recombinant DNA and enabled the assembly of gene and genome-sized synthetic products. Xenobiotic nucleic acid (XNA) analogues of DNA and RNA wi…
View article: Earth's Climate History Explains Life's Temperature Optima
Earth's Climate History Explains Life's Temperature Optima Open
Why does the growth of most life forms exhibit a narrow range of optimal temperatures below 40°C? We hypothesize that the recently identified stable range of oceanic temperatures of ~5 to 37°C for more than two billion years of Earth histo…
View article: R2D Ligase -- A novel DNA ligase with unexpected DNA-to-RNA ligation activity
R2D Ligase -- A novel DNA ligase with unexpected DNA-to-RNA ligation activity Open
DNA ligases catalyze bond formation in the backbone of nucleic acids via the formation of a phosphodiester bond between adjacent 5’ phosphates and 3’ hydroxyl groups on one strand of the duplex. While DNA ligases preferentially ligate sing…
View article: R2D ligase: Unveiling a novel DNA ligase with surprising DNA‐to‐RNA ligation activity
R2D ligase: Unveiling a novel DNA ligase with surprising DNA‐to‐RNA ligation activity Open
DNA ligases catalyze bond formation in the backbone of nucleic acids via the formation of a phosphodiester bond between adjacent 5′ phosphates and 3′ hydroxyl groups on one strand of the duplex. While DNA ligases preferentially ligate sing…
View article: Characterisation and engineering of a thermophilic RNA ligase from <i>Palaeococcus pacificus</i>
Characterisation and engineering of a thermophilic RNA ligase from <i>Palaeococcus pacificus</i> Open
RNA ligases are important enzymes in molecular biology and are highly useful for the manipulation and analysis of nucleic acids, including adapter ligation in next-generation sequencing of microRNAs. Thermophilic RNA ligases belonging to t…
View article: A role for the ATP-dependent DNA ligase Lig E of Neisseria gonorrhoeae in biofilm formation
A role for the ATP-dependent DNA ligase Lig E of Neisseria gonorrhoeae in biofilm formation Open
The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisser…
View article: A role for the ATP-dependent DNA ligase Lig E of Neisseria gonorrhoeae in biofilm formation
A role for the ATP-dependent DNA ligase Lig E of Neisseria gonorrhoeae in biofilm formation Open
The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial pathogen genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathoge…
View article: A role for the ATP-dependent DNA ligase Lig E of<i>Neisseria gonorrhoeae</i>in biofilm formation
A role for the ATP-dependent DNA ligase Lig E of<i>Neisseria gonorrhoeae</i>in biofilm formation Open
The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisser…
View article: DNA repair enzymes of the Antarctic Dry Valley metagenome
DNA repair enzymes of the Antarctic Dry Valley metagenome Open
Microbiota inhabiting the Dry Valleys of Antarctica are subjected to multiple stressors that can damage deoxyribonucleic acid (DNA) such as desiccation, high ultraviolet light (UV) and multiple freeze-thaw cycles. To identify novel or high…
View article: Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheep
Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheep Open
Antigen-specific polyclonal immunoglobulins derived from the serum, colostrum, or milk of immunized ruminant animals have potential as scalable therapeutics for the control of viral diseases including COVID-19. Here we show that the immuni…
View article: Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheep
Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheep Open
Antigen-specific polyclonal immunoglobulins derived from the serum, colostrum, or milk of immunized ruminant animals have potential as scalable therapeutics for the control of viral diseases such as COVID-19. Enhancing the efficacy of vacc…
View article: Complete Genome Sequence of a New Zealand Mycobacterium tuberculosis Strain Responsible for Ongoing Transmission over the Past 30 Years
Complete Genome Sequence of a New Zealand Mycobacterium tuberculosis Strain Responsible for Ongoing Transmission over the Past 30 Years Open
We report here the complete genome sequence of Mycobacterium tuberculosis strain Colonial S-type 1 (CS1), which has been responsible for ongoing outbreaks of tuberculosis in New Zealand over the past 30 years. CS1 appears to be highly tran…
View article: DNA ligases of<i>Prochlorococcus marinus</i>: an evolutionary exception to the rules of replication
DNA ligases of<i>Prochlorococcus marinus</i>: an evolutionary exception to the rules of replication Open
DNA ligases, essential enzymes which re-join the backbone of DNA come in two structurally-distinct isoforms, NAD-dependent and ATP-dependent, which differ in cofactor usage. The present view is that all bacteria exclusively use NAD-depende…
View article: Structural insight into DNA joining: from conserved mechanisms to diverse scaffolds
Structural insight into DNA joining: from conserved mechanisms to diverse scaffolds Open
DNA ligases are diverse enzymes with essential functions in replication and repair of DNA; here we review recent advances in their structure and distribution and discuss how this contributes to understanding their biological roles and tech…
View article: Structural intermediates of a DNA–ligase complex illuminate the role of the catalytic metal ion and mechanism of phosphodiester bond formation
Structural intermediates of a DNA–ligase complex illuminate the role of the catalytic metal ion and mechanism of phosphodiester bond formation Open
DNA ligases join adjacent 5′ phosphate (5′P) and 3′ hydroxyl (3′OH) termini of double-stranded DNA via a three-step mechanism requiring a nucleotide cofactor and divalent metal ion. Although considerable structural detail is available for …
View article: DNA binding with a minimal scaffold: structure–function analysis of Lig E DNA ligases
DNA binding with a minimal scaffold: structure–function analysis of Lig E DNA ligases Open
DNA ligases join breaks in the phosphodiester backbone of DNA by catalysing the formation of bonds between opposing 5'P and 3'OH ends in an adenylation-dependent manner. Catalysis is accompanied by reorientation of two core domains to prov…
View article: Mutational analysis of the pro‐peptide of a marine intracellular subtilisin protease supports its role in inhibition
Mutational analysis of the pro‐peptide of a marine intracellular subtilisin protease supports its role in inhibition Open
Intracellular subtilisin proteases (ISPs) have important roles in protein processing during the stationary phase in bacteria. Their unregulated protein degrading activity may have adverse effects inside a cell, but little is known about th…
View article: Discovery and characterization of a thermostable two-domain GH6 endoglucanase from a compost metagenome
Discovery and characterization of a thermostable two-domain GH6 endoglucanase from a compost metagenome Open
Enzymatic depolymerization of recalcitrant polysaccharides plays a key role in accessing the renewable energy stored within lignocellulosic biomass, and natural biodiversities may be explored to discover microbial enzymes that have evolved…
View article: Structural insight into a CE15 esterase from the marine bacterial metagenome
Structural insight into a CE15 esterase from the marine bacterial metagenome Open
The family 15 carbohydrate esterase (CE15) MZ0003, which derives from a marine Arctic metagenome, has a broader substrate scope than other members of this family. Here we report the crystal structure of MZ0003, which reveals that residues …
View article: Complete genome sequence of Halomonas sp. R5-57
Complete genome sequence of Halomonas sp. R5-57 Open
The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phe…