Adeline E. Williams
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View article: Extended phenotypes: a new generation
Extended phenotypes: a new generation Open
View article: Global genomics of<i>Aedes aegypti</i>unveils widespread and novel infectious viruses capable of triggering a small RNA response
Global genomics of<i>Aedes aegypti</i>unveils widespread and novel infectious viruses capable of triggering a small RNA response Open
The mosquito Aedes aegypti is a prominent vector for arboviruses, but the breadth of mosquito viruses that infects this specie is not fully understood. In the broadest global survey to date of over 200 Ae. aegypti small RNA samples, we det…
View article: Structural and functional comparisons of salivary α-glucosidases from the mosquito vectors Aedes aegypti, Anopheles gambiae, and Culex quinquefasciatus
Structural and functional comparisons of salivary α-glucosidases from the mosquito vectors Aedes aegypti, Anopheles gambiae, and Culex quinquefasciatus Open
View article: Transgenic Manipulation of Arthropod Vectors: Tools to Study Vector-Borne Diseases
Transgenic Manipulation of Arthropod Vectors: Tools to Study Vector-Borne Diseases Open
DiscussionBlood-feeding arthropods such as mosquitoes, ticks, and sandflies transmit several medically relevant pathogens, many of which are understudied and lack adequate therapeutics.Therefore, preventing disease transmission often requi…
View article: <i>Aedes aegypti</i> D7 long salivary proteins modulate blood feeding and parasite infection
<i>Aedes aegypti</i> D7 long salivary proteins modulate blood feeding and parasite infection Open
Mosquito saliva facilitates blood meal acquisition through pharmacologically active compounds that prevent host hemostasis and immune responses. Here, we generated two knockout (KO) mosquito lines by CRISPR/Cas9 to functionally characteriz…
View article: Quantifying Fitness Costs in Transgenic <em>Aedes aegypti</em> Mosquitoes
Quantifying Fitness Costs in Transgenic <em>Aedes aegypti</em> Mosquitoes Open
Transgenic mosquitoes often display fitness costs compared to their wild-type counterparts. In this regard, fitness cost studies involve collecting life parameter data from genetically modified mosquitoes and comparing them to mosquitoes l…
View article: Guianensin, a Simulium guianense salivary protein, has broad anti-hemostatic and anti-inflammatory properties
Guianensin, a Simulium guianense salivary protein, has broad anti-hemostatic and anti-inflammatory properties Open
Background Salivary glands from blood-feeding arthropods secrete several molecules that inhibit mammalian hemostasis and facilitate blood feeding and pathogen transmission. The salivary functions from Simulium guianense , the main vector o…
View article: Evaluation of<i>Aedes aegypti</i>Feeding Status
Evaluation of<i>Aedes aegypti</i>Feeding Status Open
Female mosquitoes need vertebrate blood for egg development. Evaluating mosquito behavior is essential for determining the ability of a mosquito to blood feed. Blood feeding experiments are often performed using artificial membrane feeders…
View article: Evaluation of<i>Aedes aegypti</i>Penetration, Probing, and Feeding Times on Mice
Evaluation of<i>Aedes aegypti</i>Penetration, Probing, and Feeding Times on Mice Open
In mosquitoes, the intradermal search for vertebrate blood (probing time) corresponds to the time taken from initial insertion of the mouthparts in the skin until visualization of the initial engorgement of blood in the midgut. Probing tim…
View article: Determination of Mosquito Probing and Feeding Time to Evaluate Mosquito Blood Feeding
Determination of Mosquito Probing and Feeding Time to Evaluate Mosquito Blood Feeding Open
Hematophagous arthropods are animals that feed on vertebrate blood for egg production. Mosquitoes must pierce the host skin, locate blood vessels, and extract blood without being noticed. Mosquito stylets lacerate host tissues, triggering …
View article: Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito <i>Aedes aegypti</i> via single-generation crosses and modeling
Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito <i>Aedes aegypti</i> via single-generation crosses and modeling Open
The yellow fever mosquito Aedes aegypti is a major vector of arthropod-borne viruses, including dengue, chikungunya, and Zika viruses. A novel approach to mitigate arboviral infections is to generate mosquitoes refractory to infection by o…
View article: Performing Immunohistochemistry in Mosquito Salivary Glands
Performing Immunohistochemistry in Mosquito Salivary Glands Open
Studying protein localization in mosquito salivary glands provides novel insights on the function and physiological relevance of salivary proteins and also provides an avenue to study interactions between mosquitoes and pathogens. Salivary…
View article: A Simple Method for Immunohistochemistry and Imaging of Mosquito Salivary Glands
A Simple Method for Immunohistochemistry and Imaging of Mosquito Salivary Glands Open
Immunohistochemistry is a valuable technique that provides information on protein localization and interactions in tissues. Mosquito salivary gland immunohistochemistry requires the meticulous dissection of a delicate tissue. The integrity…
View article: Aedes aegypti sialokinin facilitates mosquito blood feeding and modulates host immunity and vascular biology
Aedes aegypti sialokinin facilitates mosquito blood feeding and modulates host immunity and vascular biology Open
Saliva from mosquitoes contains vasodilators that antagonize vasoconstrictors produced at the bite site. Sialokinin is a vasodilator present in the saliva of Aedes aegypti. Here, we investigate its function and describe its mechanism of ac…
View article: Assessing single-component gene drive systems in the mosquito <i>Aedes aegypti</i> via single generation crosses and modeling
Assessing single-component gene drive systems in the mosquito <i>Aedes aegypti</i> via single generation crosses and modeling Open
The yellow fever mosquito Aedes aegypti is a major vector of arthropod-borne viruses, including dengue, chikungunya, and Zika. A novel approach to mitigate arboviral infections is to generate mosquitoes refractory to infection by overexpre…
View article: Aedes aegypti Piwi4 Structural Features Are Necessary for RNA Binding and Nuclear Localization
Aedes aegypti Piwi4 Structural Features Are Necessary for RNA Binding and Nuclear Localization Open
The PIWI-interacting RNA (piRNA) pathway provides an RNA interference (RNAi) mechanism known from Drosophila studies to maintain the integrity of the germline genome by silencing transposable elements (TE). Aedes aegypti mosquitoes, which …
View article: Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly’s midgut environment
Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly’s midgut environment Open
Tsetse flies are vectors of parasitic African trypanosomes, the etiological agents of human and animal African trypanosomoses. Current disease control methods include fly-repelling pesticides, fly trapping, and chemotherapeutic treatment o…
View article: Paratransgenic manipulation of tsetse<i>miR275</i>alters the physiological homeostasis of the fly’s midgut environment
Paratransgenic manipulation of tsetse<i>miR275</i>alters the physiological homeostasis of the fly’s midgut environment Open
Tsetse flies are vectors of parasitic African trypanosomes ( Trypanosoma spp.). Current disease control methods include fly-repelling pesticides, trapping flies, and chemotherapeutic treatment of infected people. Inhibiting tsetse’s abilit…
View article: The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in Transgenic Aedes aegypti
The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in Transgenic Aedes aegypti Open
The resurgence of arbovirus outbreaks across the globe, including the recent Zika virus (ZIKV) epidemic in 2015–2016, emphasizes the need for innovative vector control methods. In this study, we investigated ZIKV susceptibility to transgen…
View article: <i>Plasmodium falciparum</i> evades immunity of anopheline mosquitoes by interacting with a Pfs47 midgut receptor
<i>Plasmodium falciparum</i> evades immunity of anopheline mosquitoes by interacting with a Pfs47 midgut receptor Open
Significance The surface protein Pfs47 makes Plasmodium falciparum parasites “undetectable” to the mosquito immune system. We proposed the “lock and key” model in which a parasite expressing a compatible Pfs47 haplotype “the key,” is able …
View article: Antiviral Effectors and Gene Drive Strategies for Mosquito Population Suppression or Replacement to Mitigate Arbovirus Transmission by Aedes aegypti
Antiviral Effectors and Gene Drive Strategies for Mosquito Population Suppression or Replacement to Mitigate Arbovirus Transmission by Aedes aegypti Open
The mosquito vector Aedes aegypti transmits arthropod-borne viruses (arboviruses) of medical importance, including Zika, dengue, and yellow fever viruses. Controlling mosquito populations remains the method of choice to prevent disease tra…
View article: Engineering a Virus-Like Particle as an Antigenic Platform for a Pfs47-Targeted Malaria Transmission-Blocking Vaccine
Engineering a Virus-Like Particle as an Antigenic Platform for a Pfs47-Targeted Malaria Transmission-Blocking Vaccine Open
We recently characterized Pfs47, a protein expressed on the surface of sexual stages and ookinetes of Plasmodium falciparum , as a malaria transmission-blocking vaccine (TBV) target. Mice immunization induced antibodies that conferred stro…
View article: Antibody targeting of a specific region of Pfs47 blocks Plasmodium falciparum malaria transmission
Antibody targeting of a specific region of Pfs47 blocks Plasmodium falciparum malaria transmission Open