Adrian Drazic
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View article: A global genetic interaction map of a human cell reveals conserved principles of genetic networks
A global genetic interaction map of a human cell reveals conserved principles of genetic networks Open
We generated a genome-scale, genetic interaction network from the analysis of more than 4 million double mutants in the haploid human cell line, HAP1. The network maps ∼90,000 genetic interactions, including thousands of extreme synthetic …
View article: N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity
N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity Open
SUMMARY Most eukaryotic proteins are N-terminally acetylated, but the functional impact on a global scale has remained obscure. Using genome-wide CRISPR knockout screens in human cells, we reveal a strong genetic dependency between a major…
The Final Maturation State of β-actin Involves N-terminal Acetylation by NAA80, not N-terminal Arginylation by ATE1 Open
Actin is a hallmark protein of the cytoskeleton in eukaryotic cells, affecting a range of cellular functions. Actin dynamics is regulated through a myriad of actin-binding proteins and post-translational modifications. The mammalian actin …
Human NAA30 can rescue yeast <i>mak3∆</i> mutant growth phenotypes Open
N-terminal acetylation is an irreversible protein modification that primarily occurs co-translationally, and is catalyzed by a highly conserved family of N-terminal acetyltransferases (NATs). The NatC complex (NAA30–NAA35–NAA38) is a major…
Classification and phylogeny for the annotation of novel eukaryotic GNAT acetyltransferases Open
The enzymes of the GCN5-related N-acetyltransferase (GNAT) superfamily count more than 870 000 members through all kingdoms of life and share the same structural fold. GNAT enzymes transfer an acyl moiety from acyl coenzyme A to a wide ran…
PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin Open
The actin cytoskeleton is of profound importance to cell shape, division, and intracellular force generation. Profilins bind to globular (G-)actin and regulate actin filament formation. Although profilins are well-established actin regulat…
Classification and phylogeny for the annotation of novel eukaryotic GNAT acetyltransferases Open
The enzymes of the GCN5-related N-acetyltransferase (GNAT) superfamily count more than 870 000 members through all kingdoms of life and share the same structural fold. GNAT enzymes transfer an acyl moiety from acyl coenzyme A to a wide ran…
Mechanism of actin N-terminal acetylation Open
NAA80 Nt-acetylates the six actin isoforms by specifically targeting the complex of actin-profilin among all cellular proteins.
Actin polymerization and cell motility are affected by NAA80-mediated posttranslational N-terminal acetylation of actin Open
Actin is the most abundant protein in our cells, and also one of the most studied. Nevertheless, an important modifier of actin, the N-terminal acetyltransferase (NAT) for actin, remained unknown until now. The recent identification of the…
NAA80 is actin’s N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility Open
Significance More than 80% of human proteins are N-terminal (Nt)–acetylated during translation. In contrast, actin, the most abundant protein in the cytoplasm of animal cells, is Nt-acetylated posttranslationally and following a unique mul…
Structural determinants and cellular environment define processed actin as the sole substrate of the N-terminal acetyltransferase NAA80 Open
Significance N-terminal acetylation performed by N-terminal acetyltransferases (NATs) is a common protein modification in human cells. A unique NAT, NAA80, was recently found to control actin N-terminal acetylation and cytoskeletal dynamic…
Molecular determinants of the N-terminal acetyltransferase Naa60 anchoring to the Golgi membrane Open
Nα-Acetyltransferase 60 (Naa60 or NatF) was recently identified as an unconventional N-terminal acetyltransferase (NAT) because it localizes to organelles, in particular the Golgi apparatus, and has a preference for acetylating N termini o…
View article: The world of protein acetylation
The world of protein acetylation Open
Acetylation is one of the major post-translational protein modifications in the cell, with manifold effects on the protein level as well as on the metabolome level. The acetyl group, donated by the metabolite acetyl-coenzyme A, can be co- …