Aftab Bashir
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View article: Improving Yield and Nitrogen use Efficiency in Wheat by Overexpressing TaNAC2-5A Transcription Factor
Improving Yield and Nitrogen use Efficiency in Wheat by Overexpressing TaNAC2-5A Transcription Factor Open
TaNAC2-5A is a transcription factor that is part of the NAC family, which is associated with the regulation of plant growth and the response to stress factors, especially in terms of available nitrate. This research is focused on investiga…
View article: The transcription factor TaNF-YB4 overexpression in wheat increases plant vigor and yield
The transcription factor TaNF-YB4 overexpression in wheat increases plant vigor and yield Open
Addressing food security is a priority in developing countries. This study aimed to improve wheat yield by overexpressing the TaNF-YB4 transcription factor, which is involved in carbon assimilation and stress tolerance. An expression casse…
View article: Cotton microbiome profiling and Cotton Leaf Curl Disease (CLCuD) suppression through microbial consortia associated with Gossypium arboreum
Cotton microbiome profiling and Cotton Leaf Curl Disease (CLCuD) suppression through microbial consortia associated with Gossypium arboreum Open
The failure of breeding strategies has caused scientists to shift to other means where the new approach involves exploring the microbiome to modulate plant defense mechanisms against Cotton Leaf Curl Disease (CLCuD). The cotton microbiome …
View article: Cotton Microbiome Profiling and Cotton Leaf Curl Disease (CLCuD) Suppression through Microbial Consortia associated with<i>Gossypium arboreum</i>
Cotton Microbiome Profiling and Cotton Leaf Curl Disease (CLCuD) Suppression through Microbial Consortia associated with<i>Gossypium arboreum</i> Open
The failure of breeding strategies has caused scientists to shift to other means where the new approach involves exploring the microbiome to modulate plant defense mechanisms against CLCuD. The cotton microbiome of CLCuD-resistant varietie…
View article: Evaluation of Cis-Regulatory Elements of Dicot ?-TbSt Promoter
Evaluation of Cis-Regulatory Elements of Dicot ?-TbSt Promoter Open
The eukaryotic gene expression is controlled by a regulatory region called promoter. Many plant promoters have been characterized for regulatory motifs. There are three types of plant promoters i.e. inducible, constitutive and tissue speci…
View article: Enhanced Expression of Plasma Membrane Intrinsic Protein 2 Improves Cotton Fiber Length and Potential Economic Viability of Gossypium Arboreum
Enhanced Expression of Plasma Membrane Intrinsic Protein 2 Improves Cotton Fiber Length and Potential Economic Viability of Gossypium Arboreum Open
Background: Gossypium arboreum is a cotton crop native to tropical and subtropical regions that are naturally resistant to Cotton Leaf Curl Virus (CLCuV). However, its cultivation is unfavorable due to the lower quality and shorter fiber l…
View article: Isolation, Cloning and Characterization of a Constitutive Plant from Potato Aquaporin Gene
Isolation, Cloning and Characterization of a Constitutive Plant from Potato Aquaporin Gene Open
Plasma membrane intrinsic proteins (PIP1) are the most common integral membrane proteins belong to a larger family of intrinsic aquaporin proteins. They are member of aquaporin gene family and have gained importance as highly expressed gen…
View article: Exploration of Cotton Leaf Curl Virus (CLCuV) resistance genes and their screening in Gossypium arboreum by targeting resistance gene analogues
Exploration of Cotton Leaf Curl Virus (CLCuV) resistance genes and their screening in Gossypium arboreum by targeting resistance gene analogues Open
Cotton leaf curl virus (CLCuV) disease is one of the major limiting factors in cotton production, particularly in widely cultivated Gossypium hirsutum varieties that are susceptible to attack by this virus. Several approaches have been emp…
View article: Isolation and characterization of sucrose phosphate synthase promoter from cotton (Gossypium hirsutum L.)
Isolation and characterization of sucrose phosphate synthase promoter from cotton (Gossypium hirsutum L.) Open
Cotton fiber genes and promoters are of great importance in understanding fiber development mechanism as well as for improvement of fiber.Sucrose phosphate synthase gene (SPS) (insert abbreviation of gene)has found to express at higher rat…
View article: Histochemical GUS expression of beta tubulin promoter in transgenic tobacco
Histochemical GUS expression of beta tubulin promoter in transgenic tobacco Open
Agrobacterium mediated plant transformation is a useful technique for stable transformation of plants. This study aimed at stable transformation of the beta tubulin (β) gene promoter and its expression analysis by histochemical GUS assay. …
View article: Development of a Triple Gene Cry1Ac-Cry2Ab-EPSPS Construct and Its Expression in Nicotiana benthamiana for Insect Resistance and Herbicide Tolerance in Plants
Development of a Triple Gene Cry1Ac-Cry2Ab-EPSPS Construct and Its Expression in Nicotiana benthamiana for Insect Resistance and Herbicide Tolerance in Plants Open
Insect pest complex, cotton leaf curl disease and weeds pose major threat to crop production worldwide, including Pakistan. To address these problems, in the present study a triple gene construct harboring Cry1Ac, Cry2Ab, and EPSPS cassett…
View article: Establishment of Dipstick Development Technology for Detection of Cry1Ac in Transgenic Plants
Establishment of Dipstick Development Technology for Detection of Cry1Ac in Transgenic Plants Open
The insecticidal Bt Cry1Ac protein is, currently used for transgene expression in numerous crops or deliberating resistance against lepidopteron pests. Since the introduction of Bt cotton in Pakistan. It has been demonstrated that the tech…
View article: Cloning and expression analysis of alcohol dehydrogenase (Adh) hybrid promoter isolated from Zea mays
Cloning and expression analysis of alcohol dehydrogenase (Adh) hybrid promoter isolated from Zea mays Open
Hybrid promoters are created by shuffling of DNA fragments while keeping intact regulatory regions crucial of promoter activity. Two fragments of alcohol dehydrogenase (Adh) promoter from Zea mays were selected to generate hybrid promoter.…
View article: Stable transformation and expression of GhEXPA8 fiber expansin gene to improve fiber length and micronaire value in cotton
Stable transformation and expression of GhEXPA8 fiber expansin gene to improve fiber length and micronaire value in cotton Open
Cotton fiber is multigenic trait controlled by number of genes. Previous studies suggest that one of these genes may be responsible for switching cotton fiber growth on and off to influence the fiber quality produced from a cotton seed. In…