Kan Agarwal
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View article: Fidelity of RNA polymerase II transcription controlled by elongation factor TFIIS
Fidelity of RNA polymerase II transcription controlled by elongation factor TFIIS Open
Fidelity of DNA and protein synthesis is regulated by a proofreading mechanism but function of a similar mechanism during RNA synthesis has not been demonstrated. Analysis of transcriptional fidelity and its control has been hampered by th…
View article: Cloning, expression and characterization of the human transcription elongation factor, TFIIS
Cloning, expression and characterization of the human transcription elongation factor, TFIIS Open
The cDNA for the human elongation factor, TFIIS, has been cloned and expressed in E. coli with the T7 expression system. This 280-amino acid TFIIS protein is shorter by 21 residues than that of the mouse. The missing 21 residues are locate…
View article: Molecular structure of deoxycytidyl–3′– ethylphosphonate (RP) 5′–deoxyguanidine, d[Cp(CH3)G]. A neutral dinucleotide with Watson-Crick base pairing and a right handed helical twist
Molecular structure of deoxycytidyl–3′– ethylphosphonate (RP) 5′–deoxyguanidine, d[Cp(CH3)G]. A neutral dinucleotide with Watson-Crick base pairing and a right handed helical twist Open
The crystal structure of d[Cp(CH3)G] has been determined as part of a project to study the mechanism of the B----Z transition in DNA. The asymmetric unit contains two dinucleotides and the equivalent of 7.5 water molecules, partially disor…
View article: A gastrin gene is expressed in both porcine pituitary and antral mucosal tissues
A gastrin gene is expressed in both porcine pituitary and antral mucosal tissues Open
By employing S1 nuclease mapping of total RNA isolated from porcine cerebral cortex, cerebellum, hypothalamus, pituitary, kidney, liver, pancreas, intestine, and antral mucosa, we have investigated gastrin gene expression in these tissues.…
View article: Purification and properties of the Hpa I methylase
Purification and properties of the Hpa I methylase Open
The purification and catalytic properties of the homogeneous Hpa I methylase is described. The enzyme exists as a single polypeptide chain with a molecular weight of 37,000 +/- 2,000 was shown by sedimentation equilibrium and polyacrylamid…
View article: Synthesis and enzymatic properties of deoxyribooligonucleotides containing methyl and phenylphosphonate linkages
Synthesis and enzymatic properties of deoxyribooligonucleotides containing methyl and phenylphosphonate linkages Open
Chemical methods for the synthesis of short deoxyribooligonucleotides containing methyl and phenylphosphonodiester linkages have been developed. The interaction of two such nonionic dinucleotide analogs, T(pCH3)T and T(pC6H5)T, with severa…
View article: Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C by a modified triester method
Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C by a modified triester method Open
The synthesis of a self-complementary octanucleotide, d(G-G-T-T-A-A-C-C-), using a modified triester approach is described. The protected dinucleotides, d(Me2O)TribG(C1C6H4) ibG, d(Me2O)TrT(ClC6H4)T, d(Me2O)TrbzA(ClC6H4)bzA, and d(Me2O)Tra…