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View article: Optimized pipeline for generating highly potent neutralizing Affitins: application to SARS-CoV-2 spike protein
Optimized pipeline for generating highly potent neutralizing Affitins: application to SARS-CoV-2 spike protein Open
Viral infections represent a global health threat, causing three million deaths annually. Antiviral strategies primarily target viral genome replication or prevent virus entry into host cells. While most FDA-approved antiviral drugs are sm…
View article: Excess of guide RNA reduces knockin efficiency and drastically increases on-target large deletions
Excess of guide RNA reduces knockin efficiency and drastically increases on-target large deletions Open
CRISPR-Cas9 cleavage efficacy and accuracy are the main challenges gene editing faces, and they are particularly affected by the optimal formation of the ribonucleoprotein (RNP) complex. We used nano differential scanning fluorimetry, a la…
View article: Highly Efficient Knockin in Human iPS Cells and Rat Embryos by CRISPR/Cas9 Molecular Optimization
Highly Efficient Knockin in Human iPS Cells and Rat Embryos by CRISPR/Cas9 Molecular Optimization Open
The CRISPR/Cas9 system is now the gold standard for the generation of genetically modified cell and animal models but knockin is a bottleneck. One reason could be that there is no consensus regarding the concentrations of its components to…
View article: A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening
A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening Open
Over the past two decades, there has been a great interest in the study of HLA-E-restricted αβ T cells during bacterial and viral infections, including recently SARS-CoV-2 infection. Phenotyping of these specific HLA-E-restricted T cells r…
View article: hnRNP‐A1 binds to the IRES of MELOE‐1 antigen to promote MELOE‐1 translation in stressed melanoma cells
hnRNP‐A1 binds to the IRES of MELOE‐1 antigen to promote MELOE‐1 translation in stressed melanoma cells Open
The major challenge in antigen‐specific immunotherapy of cancer is to select the most relevant tumor antigens to target. To this aim, understanding their mode of expression by tumor cells is critical. We previously identified a melanoma‐sp…
View article: Author response for "hnRNP‐A1 binds to the IRES of MELOE‐1 antigen to promote its translation in stressed melanoma cells"
Author response for "hnRNP‐A1 binds to the IRES of MELOE‐1 antigen to promote its translation in stressed melanoma cells" Open
View article: Phase I/II clinical trial of adoptive cell transfer of sorted specific T cells for metastatic melanoma patients
Phase I/II clinical trial of adoptive cell transfer of sorted specific T cells for metastatic melanoma patients Open
View article: Cancer vaccines: designing artificial synthetic long peptides to improve presentation of class I and class II T cell epitopes by dendritic cells
Cancer vaccines: designing artificial synthetic long peptides to improve presentation of class I and class II T cell epitopes by dendritic cells Open
There is now a consensus that efficient peptide vaccination against cancer requires that peptides should (i) be exclusively presented by professional APC and (ii) stimulate both CD4 and CD8-specific T cell responses. To this aim, in recent…
View article: TCR Analyses of Two Vast and Shared Melanoma Antigen-Specific T Cell Repertoires: Common and Specific Features
TCR Analyses of Two Vast and Shared Melanoma Antigen-Specific T Cell Repertoires: Common and Specific Features Open
Among Immunotherapeutic approaches for cancer treatment, the adoptive transfer of antigen specific T cells is still a relevant approach, that could have higher efficacy when further combined with immune check-point blockade. A high number …
View article: IRES-dependent translation of the long non coding RNA <i>meloe</i> in melanoma cells produces the most immunogenic MELOE antigens
IRES-dependent translation of the long non coding RNA <i>meloe</i> in melanoma cells produces the most immunogenic MELOE antigens Open
MELOE-1 and MELOE-2, two highly specific melanoma antigens involved in T cell immunosurveillance are produced by IRES-dependent translation of the long « non coding » and polycistronic RNA, meloe. In the present study, we document the expr…