Akash Bhattacharya
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View article: Relevance of Non-Contrast Computed Tomography (NCCT) Based Alberta Stroke Program Early CT Score (ASPECTS) in Predicting Severity of Acute Ischemic Stroke at Presentation and Its Functional and Cognitive Outcome at 90 Days
Relevance of Non-Contrast Computed Tomography (NCCT) Based Alberta Stroke Program Early CT Score (ASPECTS) in Predicting Severity of Acute Ischemic Stroke at Presentation and Its Functional and Cognitive Outcome at 90 Days Open
Introduction: ASPECTS is a NCCT based topographic scoring system that provides quantitative measure of early ischemic changes. The score was initially developed for evaluating candidacy for stroke thrombolysis but currently also predicts f…
View article: Optimizing high-throughput viral vector characterization with density gradient equilibrium analytical ultracentrifugation
Optimizing high-throughput viral vector characterization with density gradient equilibrium analytical ultracentrifugation Open
Viral vector-based gene therapies and vaccines require accurate characterization of capsid species. The current gold standard for assessing capsid loading of adeno-associated virus (AAV) is sedimentation velocity analytical ultracentrifuga…
View article: Uncovering allostery and regulation in SORCIN through molecular dynamics simulations
Uncovering allostery and regulation in SORCIN through molecular dynamics simulations Open
Soluble resistance-related calcium-binding protein or Sorcin is an allosteric, calcium-binding Penta-EF hand (PEF) family protein implicated in multi-drug resistant cancers. Sorcin is known to bind chemotherapeutic molecules such as Doxoru…
View article: Redefining the role of urban studies Early Career Academics in the post-COVID-19 university
Redefining the role of urban studies Early Career Academics in the post-COVID-19 university Open
We are an international collective of Early Career Academics (ECAs) who met throughout 2020 to explore the implications of COVID-19 on precarious academics. With this intervention, our aims are to voice commonly shared experiences and conc…
View article: Virtual Screening and Molecular Docking of Flavone Derivatives as a Potential Anticancer Drug in the Presence of Dexamethasone
Virtual Screening and Molecular Docking of Flavone Derivatives as a Potential Anticancer Drug in the Presence of Dexamethasone Open
CDK6 is a pivotal kinase in cell cycle progression and has been targeted in many cancers and degenerative diseases. Fisetin, a potent flavonol inhibitor of CDK6, was modified in silico to develop better CDK6 inhibitor(s). Molecular docking…
View article: Addressing the challenges of purification and quality control in gene therapy
Addressing the challenges of purification and quality control in gene therapy Open
Recombinant adeno-associated virus (rAAV) vectors are a highly promising mechanism for therapeutic gene delivery. However, most industrial cell lines exhibit inefficient gene packaging, which results in a heterogeneous population of vector…
View article: Nucleic acid binding by SAMHD1 contributes to the antiretroviral activity and is enhanced by the GpsN modification
Nucleic acid binding by SAMHD1 contributes to the antiretroviral activity and is enhanced by the GpsN modification Open
View article: Chemotherapy or Immunotherapy: A Search for the Potential Anti-Cancer Treatment Strategies
Chemotherapy or Immunotherapy: A Search for the Potential Anti-Cancer Treatment Strategies Open
View article: Optimized filopodia formation requires myosin tail domain cooperation
Optimized filopodia formation requires myosin tail domain cooperation Open
Significance Cells interact with their environment using filopodia, thin membrane protrusions supported by actin filaments. Filopodia formation in evolutionarily divergent organisms requires force generation by a MyTH4-FERM (MF) myosin tha…
View article: A mutually induced conformational fit underlies Ca2+-directed interactions between calmodulin and the proximal C terminus of KCNQ4 K+ channels
A mutually induced conformational fit underlies Ca2+-directed interactions between calmodulin and the proximal C terminus of KCNQ4 K+ channels Open
View article: LIFE SATISFACTION AMONG MEXICAN ELDERS: FINDINGS FROM THE STUDY ON GLOBAL AGEING AND ADULT HEALTH WAVE 1
LIFE SATISFACTION AMONG MEXICAN ELDERS: FINDINGS FROM THE STUDY ON GLOBAL AGEING AND ADULT HEALTH WAVE 1 Open
Life Satisfaction (LS) is an important component of comprehensive health (WHO, 2015). Factors contributing to LS may vary by societal context. To date, limited literature has examined LS in the Mexican context and considered unique context…
View article: Functionality of Redox-Active Cysteines Is Required for Restriction of Retroviral Replication by SAMHD1
Functionality of Redox-Active Cysteines Is Required for Restriction of Retroviral Replication by SAMHD1 Open
View article: General Model for Retroviral Capsid Pattern Recognition by TRIM5 Proteins
General Model for Retroviral Capsid Pattern Recognition by TRIM5 Proteins Open
Restriction factors are intrinsic cellular defense proteins that have evolved to block microbial infections. Retroviruses such as HIV-1 are restricted by TRIM5 proteins, which recognize the viral capsid shell that surrounds, organizes, and…
View article: Uncovering allostery and regulation in SAMHD1 through molecular dynamics simulations
Uncovering allostery and regulation in SAMHD1 through molecular dynamics simulations Open
Originally published in PROTEINS: Structure, Function, and Bioinformatics 2017;85(7):1266–1275(DOI: 10.1002/prot.25287) There was an error in the author's affiliations. The correct affiliations are listed below. Kajwal Kumar Patra, Departm…
View article: Novel Insights from Structural Analysis of Interactions of KCNQ K + Channels with Calmodulin
Novel Insights from Structural Analysis of Interactions of KCNQ K + Channels with Calmodulin Open
View article: MicroRNA Regulation of Oxidative Stress
MicroRNA Regulation of Oxidative Stress Open
View article: The small‐molecule 3G11 inhibits HIV‐1 reverse transcription
The small‐molecule 3G11 inhibits HIV‐1 reverse transcription Open
The small‐molecule 6‐( tert ‐butyl)‐4‐phenyl‐4‐(trifluoromethyl)‐1H,3H‐1,3,5‐triazin‐2‐one (3G11) inhibits HIV‐1 replication in the human T cell line MT‐2. Here, we showed that 3G11 specifically and potently blocks HIV‐1 infection. By cont…
View article: Allosteric dynamics of SAMHD1 studied by molecular dynamics simulations
Allosteric dynamics of SAMHD1 studied by molecular dynamics simulations Open
SAMHD1 is a human cellular enzyme that blocks HIV-1 infection in myeloid cells and non-cycling CD4+T cells. The enzyme is an allosterically regulated triphosphohydrolase that modulates the level of cellular dNTP. The virus restriction is a…
View article: Allosteric Activation of SAMHD1 Protein by Deoxynucleotide Triphosphate (dNTP)-dependent Tetramerization Requires dNTP Concentrations That Are Similar to dNTP Concentrations Observed in Cycling T Cells
Allosteric Activation of SAMHD1 Protein by Deoxynucleotide Triphosphate (dNTP)-dependent Tetramerization Requires dNTP Concentrations That Are Similar to dNTP Concentrations Observed in Cycling T Cells Open
SAMHD1 is a dNTP hydrolase, whose activity is required for maintaining low dNTP concentrations in non-cycling T cells, dendritic cells, and macrophages. SAMHD1-dependent dNTP depletion is thought to impair retroviral replication in these c…
View article: Effects of T592 phosphomimetic mutations on tetramer stability and dNTPase activity of SAMHD1 can not explain the retroviral restriction defect
Effects of T592 phosphomimetic mutations on tetramer stability and dNTPase activity of SAMHD1 can not explain the retroviral restriction defect Open
View article: HTS screening using NCI diversity set V compounds library.
HTS screening using NCI diversity set V compounds library. Open
(A) Affinity of C-fos to Full length HuR. The Kd was derived by fitting to the equation of ΔP = ΔPmax*[HuR]/(Kd+[HuR])[46]. (B) Competition assay using unlabeled C-fos RNA. IC50s were obtained by fitting to the equation of ΔP = ΔPmax/(1+10…
View article: Identification of Small-Molecule Inhibitors of the HuR/RNA Interaction Using a Fluorescence Polarization Screening Assay Followed by NMR Validation
Identification of Small-Molecule Inhibitors of the HuR/RNA Interaction Using a Fluorescence Polarization Screening Assay Followed by NMR Validation Open
The human antigen R (HuR) stabilizes many mRNAs of proto-oncogene, transcription factors, cytokines and growth factors by recognizing AU-rich elements (AREs) presented in their 3' or 5' untranslated region (UTR). Multiple lines of experime…
View article: Modulating RNA Alignment Using Directional Dynamic Kinks: Application in Determining an Atomic-Resolution Ensemble for a Hairpin using NMR Residual Dipolar Couplings
Modulating RNA Alignment Using Directional Dynamic Kinks: Application in Determining an Atomic-Resolution Ensemble for a Hairpin using NMR Residual Dipolar Couplings Open
Approaches that combine experimental data and computational molecular dynamics (MD) to determine atomic resolution ensembles of biomolecules require the measurement of abundant experimental data. NMR residual dipolar couplings (RDCs) carry…
View article: A RANDOMIZED CONTROLLED TRIAL TO EVALUATE THE ANTI-INFLAMMATORY EFFECT OF COMMIPHORA WIGHTII IN COMBINATION WITH CURCUMA LONGA AGAINST DICLOFENAC POTASSIUM IN NON-SPECIFIC KNEE EFFUSION
A RANDOMIZED CONTROLLED TRIAL TO EVALUATE THE ANTI-INFLAMMATORY EFFECT OF COMMIPHORA WIGHTII IN COMBINATION WITH CURCUMA LONGA AGAINST DICLOFENAC POTASSIUM IN NON-SPECIFIC KNEE EFFUSION Open
Knee effusion is an abnormal accumulation of the fluid in the knee capsule or the adjoining supra-patellar bursa secondary to the irritation/inflammation in the joint synovium.It is seen in approximately 8-10 % of the total patients suffer…