Alan J. Stewart
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View article: Investigating the conformational landscape of human histidine-rich glycoprotein using amide HDX-MS
Investigating the conformational landscape of human histidine-rich glycoprotein using amide HDX-MS Open
Histidine-rich glycoprotein (HRG) is a multidomain plasma protein involved in immune modulation, angiogenesis, coagulation and fibrinolysis. Despite its broad biological relevance, structural investigations into HRG have yielded only limit…
View article: Abnormal Plasma/Serum Magnesium, Copper, and Zinc Concentrations Associate with the Future Development of Cardiovascular Diseases
Abnormal Plasma/Serum Magnesium, Copper, and Zinc Concentrations Associate with the Future Development of Cardiovascular Diseases Open
Background/Objectives: Cardiovascular diseases (CVDs) are the leading cause of global mortality. Major adverse cardiovascular events (MACEs)—such as acute myocardial infarction, stroke, and heart failure—are critical endpoints in the clini…
View article: Quantitative proteomics identifies plasma protein alterations that associate with metabolic and thrombotic profile changes after bariatric surgery
Quantitative proteomics identifies plasma protein alterations that associate with metabolic and thrombotic profile changes after bariatric surgery Open
Objective Roux‐en‐Y gastric bypass (RYGB) surgery has been shown to lead to favourable health outcomes in obese patients. However, the molecular changes that occur and how they relate to clinical measures are poorly understood. Here, we ch…
View article: Properties of the major Zn<sup>2+</sup>-binding site of human alpha-fetoprotein, a potential foetal plasma zinc carrier
Properties of the major Zn<sup>2+</sup>-binding site of human alpha-fetoprotein, a potential foetal plasma zinc carrier Open
The foetal plasma protein alpha-fetoprotein harbours a strong Zn 2+ binding site ( K D = 1.9 × 10 −8 M), supporting the hypothesis of a role in foetal zinc homeostasis.
View article: Metallomic analysis of urine from individuals with and without Covid-19 infection reveals extensive alterations in metal homeostasis
Metallomic analysis of urine from individuals with and without Covid-19 infection reveals extensive alterations in metal homeostasis Open
Metal ions perform important functions in the body and their concentrations in cells and tissues are tightly controlled. Alterations in metal homeostasis can occur in certain disease states including infection. In this study urinary excret…
View article: OP3 Identification and examination of Mitsugumin 23 within platelets
OP3 Identification and examination of Mitsugumin 23 within platelets Open
Intracellular zinc and calcium dynamics are important for both platelet activation and function. We have recently shown that the Ca2+ permeable cation channel mitsugumin 23 (MG23), found in the endoplasmic/sarcoplasmic reticulum of other …
View article: Recent Advances in Metalloproteomics
Recent Advances in Metalloproteomics Open
Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine, and toxicology. Despite the involvement of essential elements in all major processes necessa…
View article: EPR spectroscopic characterisation of native Cu<sup>II</sup>-binding sites in human serum albumin
EPR spectroscopic characterisation of native Cu<sup>II</sup>-binding sites in human serum albumin Open
Using a suite of electron paramagnetic resonance spectroscopy techniques to investigate single-histidine knockout mutants of human serum albumin, a set of three histidine residues can be determined as primary and secondary copper ion bindi…
View article: Zn2+ Differentially Influences the Neutralisation of Heparins by HRG, Fibrinogen, and Fibronectin
Zn2+ Differentially Influences the Neutralisation of Heparins by HRG, Fibrinogen, and Fibronectin Open
For coagulation to be initiated, anticoagulant glycosaminoglycans (GAGs) such as heparins need to be neutralised to allow fibrin clot formation. Platelet activation triggers the release of several proteins that bind GAGs, including histidi…
View article: Editorial: Insulin resistance and cardiovascular disease
Editorial: Insulin resistance and cardiovascular disease Open
Peer reviewed
View article: The role of Zn2+ in shaping intracellular Ca2+ dynamics in the heart
The role of Zn2+ in shaping intracellular Ca2+ dynamics in the heart Open
Increasing evidence suggests that Zn2+ acts as a second messenger capable of transducing extracellular stimuli into intracellular signaling events. The importance of Zn2+ as a signaling molecule in cardiovascular functioning is gaining tra…
View article: Magnesium Deficiency and Cardiometabolic Disease
Magnesium Deficiency and Cardiometabolic Disease Open
Magnesium (Mg2+) has many physiological functions within the body. These include important roles in maintaining cardiovascular functioning, where it contributes to the regulation of cardiac excitation–contraction coupling, endothelial func…
View article: Investigating Native Metal Ion Binding Sites in Mammalian Histidine-Rich Glycoprotein
Investigating Native Metal Ion Binding Sites in Mammalian Histidine-Rich Glycoprotein Open
Mammalian histidine-rich glycoprotein (HRG) is a highly versatile and abundant blood plasma glycoprotein with a diverse range of ligands that is involved in regulating many essential biological processes, including coagulation, cell adhesi…
View article: Investigating Native Metal Ion Binding Sites in Mammalian Histidine-Rich Glycoprotein
Investigating Native Metal Ion Binding Sites in Mammalian Histidine-Rich Glycoprotein Open
Mammalian histidine-rich glycoprotein (HRG) is a highly versatile and abundant blood plasma glycoprotein with a diverse range of ligands that is involved in regulating many essential biological processes, including coagulation, cell adhesi…
View article: Optimised plasma sample preparation and LC‐MS analysis to support large‐scale proteomic analysis of clinical trial specimens: Application to the Fenofibrate Intervention and Event Lowering in Diabetes (FIELD) trial
Optimised plasma sample preparation and LC‐MS analysis to support large‐scale proteomic analysis of clinical trial specimens: Application to the Fenofibrate Intervention and Event Lowering in Diabetes (FIELD) trial Open
Purpose Robust, affordable plasma proteomic biomarker workflows are needed for large‐scale clinical studies. We evaluated aspects of sample preparation to allow liquid chromatography‐mass spectrometry (LC‐MS) analysis of more than 1500 sam…
View article: Investigating Native Metal Ion Binding Sites in Mammalian Histidine-Rich Glycoprotein
Investigating Native Metal Ion Binding Sites in Mammalian Histidine-Rich Glycoprotein Open
Mammalian histidine-rich glycoprotein (HRG) is a highly versatile and abundant blood plasma glycoprotein with a diverse range of ligands that is involved in regulating many essential biological processes, including coagulation, cell adhesi…
View article: Structural and biochemical characterisation of Co<sup>2+</sup>-binding sites on serum albumins and their interplay with fatty acids
Structural and biochemical characterisation of Co<sup>2+</sup>-binding sites on serum albumins and their interplay with fatty acids Open
Serum albumin–Co 2+ interactions are of clinical importance.
View article: Periarticular metal hypersensitivity complications of hip bearings containing cobalt–chromium
Periarticular metal hypersensitivity complications of hip bearings containing cobalt–chromium Open
Hip joints with bearings composed of cobalt–chromium alloy (metal-on-metal bearings) have been one of the most widely used implants in joint replacement arthroplasty. Unfortunately, these implants can contribute to a complication called as…
View article: Strategies for Therapeutic Amelioration of Aberrant Plasma Zn2+ Handling in Thrombotic Disease: Targeting Fatty Acid/Serum Albumin-Mediated Effects
Strategies for Therapeutic Amelioration of Aberrant Plasma Zn2+ Handling in Thrombotic Disease: Targeting Fatty Acid/Serum Albumin-Mediated Effects Open
The initiation, maintenance and regulation of blood coagulation is inexorably linked to the actions of Zn2+ in blood plasma. Zn2+ interacts with a variety of haemostatic proteins in the bloodstream including fibrinogen, histidine-rich glyc…
View article: Organism-specific differences in the binding of ketoprofen to serum albumin
Organism-specific differences in the binding of ketoprofen to serum albumin Open
Serum albumin is a circulatory transport protein that has a highly conserved sequence and structure across mammalian organisms. Its ligand-binding properties are of importance as albumin regulates the pharmacokinetics of many drugs. Due to…
View article: Identification of plasma proteins associated with oesophageal cancer chemotherapeutic treatment outcomes using SWATH-MS
Identification of plasma proteins associated with oesophageal cancer chemotherapeutic treatment outcomes using SWATH-MS Open
Oesophageal adenocarcinoma (OAC) is an aggressive cancer with a five-year survival of <15%. Current chemotherapeutic strategies only benefit a minority (20-30%) of patients and there are no methods available to differentiate between respon…
View article: Total plasma magnesium, zinc, copper and selenium concentrations in obese patients before and after bariatric surgery
Total plasma magnesium, zinc, copper and selenium concentrations in obese patients before and after bariatric surgery Open
Obesity enhances the risk of type-2 diabetes, cardiovascular disease and inflammatory conditions and often leads to metal dyshomeostasis, which contributes to the negative health aspects associated with the disease. In severe cases, bariat…
View article: Albumin-mediated extracellular zinc speciation drives cellular zinc uptake
Albumin-mediated extracellular zinc speciation drives cellular zinc uptake Open
Fatty acids alter zinc speciation in plasma, increasing zinc influx into endothelial cells.