Alan Sloma
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View article: Hyaluronic Acid Production in <i>Bacillus subtilis</i>
Hyaluronic Acid Production in <i>Bacillus subtilis</i> Open
The hasA gene from Streptococcus equisimilis , which encodes the enzyme hyaluronan synthase, has been expressed in Bacillus subtilis , resulting in the production of hyaluronic acid (HA) in the 1-MDa range. Artificial operons were assemble…
View article: Extracellular Proteolytic Activity Plays a Central Role in Swarming Motility in<i>Bacillus subtilis</i>
Extracellular Proteolytic Activity Plays a Central Role in Swarming Motility in<i>Bacillus subtilis</i> Open
Natural isolates of Bacillus subtilis exhibit a robust multicellular behavior known as swarming. A form of motility, swarming is characterized by a rapid, coordinated progression of a bacterial population across a surface. As a collective …
View article: Microarray analysis of the <i>Bacillus subtilis</i> K‐state: genome‐wide expression changes dependent on ComK
Microarray analysis of the <i>Bacillus subtilis</i> K‐state: genome‐wide expression changes dependent on ComK Open
Summary In Bacillus subtilis , the competence transcription factor ComK activates its own transcription as well as the transcription of genes that encode DNA transport proteins. ComK is expressed in about 10% of the cells in a culture grow…
View article: Aminopeptidase from Sphingomonas capsulata
Aminopeptidase from Sphingomonas capsulata Open
A novel aminopeptidase with unique substrate specificity was purified from a culture broth of Sphingomonas capsulata. This is the first reported aminopeptidase to demonstrate broad substrate specificity and yet release glycine and alanine …
View article: Development of marker-free strains of Bacillus subtilis capable of secreting high levels of industrial enzymes
Development of marker-free strains of Bacillus subtilis capable of secreting high levels of industrial enzymes Open
Different strategies have been employed to achieve high-level expression of single-copy genes encoding secreted enzymes in Bacillus subtilis. A model system was developed which utilizes the aprL gene from Bacillus clausii as a reporter gen…
View article: Genetic engineering of Bacillus subtilis for the commercial production of riboflavin
Genetic engineering of Bacillus subtilis for the commercial production of riboflavin Open
Recombinant DNA engineering was combined with mutant selection and fermentation improvement to develop a strain of Bacillus subtilis that produces commercially attractive levels of riboflavin. The B. subtilis riboflavin production strain c…
View article: Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis
Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis Open
We have purified a minor extracellular serine protease from a strain of Bacillus subtilis bearing null mutations in five extracellular protease genes: apr, npr, epr, bpr, and mpr (A. Sloma, C. Rudolph, G. Rufo, Jr., B. Sullivan, K. Theriau…
View article: Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene
Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene Open
We have purified a minor extracellular serine protease from Bacillus subtilis. Characterization of this enzyme indicated that it was most likely the previously reported enzyme bacillopeptidase F. The amino-terminal sequence of the purified…
View article: Isolation and characterization of a novel extracellular metalloprotease from Bacillus subtilis
Isolation and characterization of a novel extracellular metalloprotease from Bacillus subtilis Open
We have isolated and characterized two minor extracellular proteases from culture supernatants of a strain of Bacillus subtilis containing deletion mutations of the genes for the extracellular proteases subtilisin (apr) and neutral proteas…
View article: Gene encoding a novel extracellular metalloprotease in Bacillus subtilis
Gene encoding a novel extracellular metalloprotease in Bacillus subtilis Open
The gene for a novel extracellular metalloprotease was cloned, and its nucleotide sequence was determined. The gene (mpr) encodes a primary product of 313 amino acids that has little similarity to other known Bacillus proteases. The amino …
View article: Gene encoding a minor extracellular protease in Bacillus subtilis
Gene encoding a minor extracellular protease in Bacillus subtilis Open
The gene for a minor, extracellular protease has been identified in Bacillus subtilis. The gene (epr) encoded a primary product of 645 amino acids that was partially homologous to both subtilisin (Apr) and the major internal serine proteas…
View article: Molecular cloning and nucleotide sequence of the type I β-lactamase gene from<i>Bacillus cereus</i>
Molecular cloning and nucleotide sequence of the type I β-lactamase gene from<i>Bacillus cereus</i> Open
The gene for the type I beta-lactamase from Bacillus cereus has been cloned in Bacillus subtilis and Escherichia coli. In B. subtilis, penicillinase activity is detected and the enzyme is secreted. In E. coli, the gene confers ampicillin r…
View article: Construction and identification of bacterial plasmids containing nucleotide sequence for human leukocyte interferon.
Construction and identification of bacterial plasmids containing nucleotide sequence for human leukocyte interferon. Open
Bacterial plasmids containing human leukocyte interferon sequences were constructed and identified. Identification was confirmed by correspondence of the nucleotide sequence with out amino acid sequence of human leukocyte interferon. The f…
View article: Synthesis of human fibroblast interferon by E. coli
Synthesis of human fibroblast interferon by E. coli Open
A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonu…