Alejandro Gómez Toledo
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View article: Multi-omic analysis reveals the unique glycan landscape of the blood-brain barrier glycocalyx
Multi-omic analysis reveals the unique glycan landscape of the blood-brain barrier glycocalyx Open
The blood-brain barrier (BBB) glycocalyx is the dense layer of glycans and glycoconjugates that coats the luminal surface of the central nervous system (CNS) vasculature. Despite being the first point of contact between the blood and brain…
View article: Cumulating MS Signal enables polyclonal antibody analysis
Cumulating MS Signal enables polyclonal antibody analysis Open
Unraveling the complexities of protein systems via Mass Spectrometry (MS), particularly polyclonal antibodies, demands innovative analytical strategies. Here, we introduce the cumulative MS score (cMS), a novel mathematical framework that …
View article: Endothelial Glycocalyx Turnover in Vascular Health and Disease: Rethinking Endothelial Dysfunction
Endothelial Glycocalyx Turnover in Vascular Health and Disease: Rethinking Endothelial Dysfunction Open
The endothelial glycocalyx, a glycan-rich layer on the luminal surface of endothelial cells lining blood and lymphatic vessels, plays a crucial role in vascular homeostasis by regulating vascular permeability, immune cell trafficking, and …
View article: Dissecting the properties of circulating IgG against streptococcal pathogens through a combined systems antigenomics-serology workflow
Dissecting the properties of circulating IgG against streptococcal pathogens through a combined systems antigenomics-serology workflow Open
View article: Multiplex genome editing eliminates lactate production without impacting growth rate in mammalian cells
Multiplex genome editing eliminates lactate production without impacting growth rate in mammalian cells Open
View article: Multiplex genome editing eliminates the Warburg Effect without impacting growth rate in mammalian cells
Multiplex genome editing eliminates the Warburg Effect without impacting growth rate in mammalian cells Open
The Warburg effect is ubiquitous in proliferative mammalian cells, including cancer cells, but poses challenges for biopharmaceutical production, as lactate accumulation inhibits cell growth and protein production. Previous efforts to elim…
View article: The hinge-engineered IgG1-IgG3 hybrid subclass IgGh47 potently enhances Fc-mediated function of anti-streptococcal and SARS-CoV-2 antibodies
The hinge-engineered IgG1-IgG3 hybrid subclass IgGh47 potently enhances Fc-mediated function of anti-streptococcal and SARS-CoV-2 antibodies Open
View article: Dissecting the properties of circulating IgG against Group A Streptococcus through a combined systems antigenomics-serology workflow
Dissecting the properties of circulating IgG against Group A Streptococcus through a combined systems antigenomics-serology workflow Open
Most individuals maintain circulating antibodies against various pathogenic bacteria as a consequence of previous exposures. However, it remains unclear to what extent these antibodies contribute to host protection. This knowledge gap is l…
View article: Pathogen-driven degradation of endogenous and therapeutic antibodies during streptococcal infections
Pathogen-driven degradation of endogenous and therapeutic antibodies during streptococcal infections Open
Group A streptococcus (GAS) is a major bacterial pathogen responsible for both local and systemic infections in humans. The molecular mechanisms that contribute to disease heterogeneity remain poorly understood. Here we show that the trans…
View article: Release of extracellular matrix components after human traumatic brain injury
Release of extracellular matrix components after human traumatic brain injury Open
Most research on the evolution of damage after traumatic brain injury (TBI) focuses on cellular effects, but the analysis of human tissue slices and animal research have shown that TBI causes concomitant damage in the extracellular matrix,…
View article: Vascular proteome responses precede organ dysfunction in sepsis
Vascular proteome responses precede organ dysfunction in sepsis Open
Vascular dysfunction and organ failure are two distinct, albeit highly interconnected clinical outcomes linked to morbidity and mortality in human sepsis. The mechanisms driving vascular and parenchymal damage are dynamic and display signi…
View article: The specificity of the malarial VAR2CSA protein for chondroitin sulfate depends on 4-O-sulfation and ligand accessibility
The specificity of the malarial VAR2CSA protein for chondroitin sulfate depends on 4-O-sulfation and ligand accessibility Open
View article: A Systems View of the Heparan Sulfate Interactome
A Systems View of the Heparan Sulfate Interactome Open
Heparan sulfate proteoglycans consist of a small family of proteins decorated with one or more covalently attached heparan sulfate glycosaminoglycan chains. These chains have intricate structural patterns based on the position of sulfate g…
View article: Terminally sialylated and fucosylated complex N-glycans are involved in the malignant behavior of high-grade glioma
Terminally sialylated and fucosylated complex N-glycans are involved in the malignant behavior of high-grade glioma Open
Gliomas are the most common intracranial primary tumors, for which very few therapeutic options are available. The most malignant subtype is the glioblastoma, a disease associated with a 5-year survival rate lower than 5%. Given that resea…
View article: Beware, commercial chondroitinases vary in activity and substrate specificity
Beware, commercial chondroitinases vary in activity and substrate specificity Open
Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease. The most common method to analyze their…
View article: Proteomic atlas of organ vasculopathies triggered by Staphylococcus aureus sepsis
Proteomic atlas of organ vasculopathies triggered by Staphylococcus aureus sepsis Open
View article: Expanding the chondroitin glycoproteome of Caenorhabditis elegans
Expanding the chondroitin glycoproteome of Caenorhabditis elegans Open
Chondroitin sulfate proteoglycans (CSPGs) are important structural components of connective tissues in essentially all metazoan organisms. In vertebrates, CSPGs are involved also in more specialized processes such as neurogenesis and growt…
View article: Site-specific identification of heparan and chondroitin sulfate glycosaminoglycans in hybrid proteoglycans
Site-specific identification of heparan and chondroitin sulfate glycosaminoglycans in hybrid proteoglycans Open
Heparan sulfate (HS) and chondroitin sulfate (CS) are complex polysaccharides that regulate important biological pathways in virtually all metazoan organisms. The polysaccharides often display opposite effects on cell functions with HS and…