Aleksandra Anna Zasada
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View article: Development of an in vitro method to assess the immunogenicity of biologics in the prevention of infectious diseases
Development of an in vitro method to assess the immunogenicity of biologics in the prevention of infectious diseases Open
We present a series of preclinical studies focused on developing in vitro 2D and 3D models for assessing immunogenic factors in preventing infectious diseases. Human peripheral blood mononuclear cells (PBMC) and Calu-3 cell lines (bronchia…
View article: Two diphtheria sub-clusters with autochthonous cases in Germany and Poland within a Corynebacterium diphtheriae ST-574 outbreak, 2022 to July 2025
Two diphtheria sub-clusters with autochthonous cases in Germany and Poland within a Corynebacterium diphtheriae ST-574 outbreak, 2022 to July 2025 Open
Within a Corynebacterium diphtheriae ST-574 outbreak comprising mostly migration-associated cases since 2022, Germany detected two sub-clusters in 2025. Among sub-cluster cases with travel information (24/26), 22 were acquired autochthonou…
View article: A case of fatal respiratory diphtheria imported from Poland to Germany: possible link to an undetected imported diphtheria cluster in Poland?
A case of fatal respiratory diphtheria imported from Poland to Germany: possible link to an undetected imported diphtheria cluster in Poland? Open
NGS data suggest a common source of infection in Poland and a possible link to the Europe-wide outbreak of imported diphtheria with C. diphtheriae since 2022. This is the first diphtheria case in Poland since 2000.
View article: Passive Immunisation in the Treatment of Infectious Diseases Related to Highly Potent Bacterial Toxins
Passive Immunisation in the Treatment of Infectious Diseases Related to Highly Potent Bacterial Toxins Open
The discovery of microbial toxins as the primary factors responsible for disease manifestations and the discovery that these toxins could be neutralised by antitoxins are linked to the birth of immunology. In the late 19th century, the ser…
View article: Challenges of Diphtheria Toxin Detection
Challenges of Diphtheria Toxin Detection Open
Diphtheria toxin (DT) is the main virulence factor of Corynebacterium diphtheriae, C. ulcerans and C. pseudotuberculosis. Moreover, new Corynebacterium species with the potential to produce diphtheria toxin have also been described. Theref…
View article: The whole genome sequence of Polish vaccine strain <i>Mycobacterium bovis</i> BCG Moreau
The whole genome sequence of Polish vaccine strain <i>Mycobacterium bovis</i> BCG Moreau Open
Currently, tuberculosis immunoprophylaxis is based solely on Bacillus Calmette-Guérin (BCG) vaccination, and some of the new potential tuberculosis vaccines are based on the BCG genome. Therefore, it is reasonable to analyze the genomes of…
View article: Multidrug resistance of Escherichia coli isolated from the urinary bladder of dogs and cats with suspected urinary tract infections
Multidrug resistance of Escherichia coli isolated from the urinary bladder of dogs and cats with suspected urinary tract infections Open
High resistance to aztreonam (61% and 32% of isolates from dogs and cats, respectively), other beta-lactams, and fluoroquinolones should cause be alarm due to zoonotic potential and cross-transmission of antimicrobial-resistant microorgani…
View article: Analysis of apoE levels in Alzheimer’s disease patients and control subjects of APOE ε4 variant carriers and non‐carriers
Analysis of apoE levels in Alzheimer’s disease patients and control subjects of APOE ε4 variant carriers and non‐carriers Open
Background Alzheimer’s disease (AD) is the most common progressive dementia disease in people over 60 years of age. The pathogenesis of AD is thought to be complex and depends on environmental and genetic factors. The most important geneti…
View article: Novel combinatorial therapy of oncolytic adenovirus AdV5/3-D24-ICOSL-CD40L with anti PD-1 exhibits enhanced anti-cancer efficacy through promotion of intratumoral T-cell infiltration and modulation of tumour microenvironment in mesothelioma mouse model
Novel combinatorial therapy of oncolytic adenovirus AdV5/3-D24-ICOSL-CD40L with anti PD-1 exhibits enhanced anti-cancer efficacy through promotion of intratumoral T-cell infiltration and modulation of tumour microenvironment in mesothelioma mouse model Open
Introduction Malignant mesothelioma is a rare and aggressive form of cancer. Despite improvements in cancer treatment, there are still no curative treatment modalities for advanced stage of the malignancy. The aim of this study was to eval…
View article: COVID-19 Vaccines over Three Years after the Outbreak of the COVID-19 Epidemic
COVID-19 Vaccines over Three Years after the Outbreak of the COVID-19 Epidemic Open
The outbreak of COVID-19 started in December 2019 and spread rapidly all over the world. It became clear that the development of an effective vaccine was the only way to stop the pandemic. It was the first time in the history of infectious…
View article: Adjuvant Effect of Whole-Cell Pertussis Component on Tetanus Toxoid Potency in Murine Model
Adjuvant Effect of Whole-Cell Pertussis Component on Tetanus Toxoid Potency in Murine Model Open
There is currently an increasing interest in the development of new-generation purified antigen-based vaccines with a higher safety profile compared to conventional inactivated vaccines. The main problem of subunit vaccines is their lower …
View article: Analysis of genetic variants related to oxidative stress in patients with Alzheimer’s disease
Analysis of genetic variants related to oxidative stress in patients with Alzheimer’s disease Open
Background Alzheimer’s disease (AD) is one of the common neurodegenerative diseases with an incompletely understood pathomechanism. In AD, generation of oxidative stress is observed as a result of dysfunction of the mitochondrial respirato…
View article: Comparison of Kinetics of Antibody Avidity and IgG Subclasses’ Response in Patients with COVID-19 and Healthy Individuals Vaccinated with the BNT162B2 (Comirnaty, Pfizer/BioNTech) mRNA Vaccine
Comparison of Kinetics of Antibody Avidity and IgG Subclasses’ Response in Patients with COVID-19 and Healthy Individuals Vaccinated with the BNT162B2 (Comirnaty, Pfizer/BioNTech) mRNA Vaccine Open
There are limited reports concerning the levels of antibodies in IgG subclasses and the avidity of IgG, which is the functional strength with which an antibody binds to an antigen in serum samples obtained at different times after infectio…
View article: Genomic Analysis of Corynebacterium diphtheriae Strains Isolated in the Years 2007–2022 with a Report on the Identification of the First Non-Toxigenic tox Gene-Bearing Strain in Poland
Genomic Analysis of Corynebacterium diphtheriae Strains Isolated in the Years 2007–2022 with a Report on the Identification of the First Non-Toxigenic tox Gene-Bearing Strain in Poland Open
Infections caused by non-toxigenic Corynebacterium diphtheriae have been reported every year in Poland since 2004, with the ST8 biovar gravis strains being most commonly isolated. This study analyzed thirty strains isolated between 2017 an…
View article: New Corynebacterium Species with the Potential to Produce Diphtheria Toxin
New Corynebacterium Species with the Potential to Produce Diphtheria Toxin Open
Only three Corynebacterium species are known to produce a lethal exotoxin called diphtheria toxin. These are C. diphtheriae, C. ulcerans and C. pseudotuberculosis. The diphtheria toxin gene (tox) is carried in a family of closely related c…
View article: Detection of SARS-CoV-2 Using Reverse Transcription Helicase Dependent Amplification and Reverse Transcription Loop-Mediated Amplification Combined with Lateral Flow Assay
Detection of SARS-CoV-2 Using Reverse Transcription Helicase Dependent Amplification and Reverse Transcription Loop-Mediated Amplification Combined with Lateral Flow Assay Open
Rapid and accurate detection and identification of pathogens in clinical samples is essential for all infection diseases. However, in the case of epidemics, it plays a key role not only in the implementation of effective therapy but also i…
View article: Evaluation of precipitation time of the aluminum salts adsorbed potentially frozen vaccines used in the Polish National Immunization Schedule for their pre-qualification before the administration
Evaluation of precipitation time of the aluminum salts adsorbed potentially frozen vaccines used in the Polish National Immunization Schedule for their pre-qualification before the administration Open
The finding of the study can be used in practice by the personnel administering vaccines to patients. Step-by-step recommendations for the preparation of the test have been proposed in the article.
View article: Tandem Olefin Metathesis/α-Ketohydroxylation Revisited
Tandem Olefin Metathesis/α-Ketohydroxylation Revisited Open
EWG-activated and polar quaternary ammonium salt-tagged ruthenium metathesis catalysts have been applied in a two-step one-pot metathesis-oxidation process leading to functionalized α-hydroxyketones (acyloins). In this assisted tandem proc…
View article: Application of Loop-Mediated Isothermal Amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheriae
Application of Loop-Mediated Isothermal Amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheriae Open
Background Diphtheria outbreaks occurred in endemic areas and imported and indigenous cases are reported in UE/EEA. Because of the high infectiveness and severity of the disease, early and accurate diagnosis of each suspected case is essen…
View article: Application of Loop-Mediated Isothermal Amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheriae
Application of Loop-Mediated Isothermal Amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheriae Open
Background Diphtheria outbreaks occurred in endemic areas and imported and indigenous cases are reported in UE/EEA. Because of the high infectiveness and severity of the disease, early and accurate diagnosis of each suspected case is essen…
View article: Application of Loop-Mediated Isothermal Amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheriae
Application of Loop-Mediated Isothermal Amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheriae Open
Background: Diphtheria outbreaks occurred in endemic areas and imported and indigenous cases are reported in UE/EEA. Because of the high infectiveness and severity of the disease, early and accurate diagnosis of each suspected case is esse…
View article: Application of Loop-Mediated Isothermal Amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheriae
Application of Loop-Mediated Isothermal Amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheriae Open
Background Diphtheria outbreaks occurred in endemic areas and imported and indigenous cases are reported in UE/EEA. Because of the high infectiveness and severity of the disease, early and accurate diagnosis of each suspected case is essen…
View article: Detection and Identification of Bacillus anthracis: From Conventional to Molecular Microbiology Methods
Detection and Identification of Bacillus anthracis: From Conventional to Molecular Microbiology Methods Open
Rapid and reliable identification of Bacillus anthracis is of great importance, especially in the event of suspected deliberate release of anthrax spores. However, the identification of B. anthracis is challenging due to its high similarit…
View article: Causes Of Pertussis Incidence Increase
Causes Of Pertussis Incidence Increase Open
Bordetella pertussis is an etiological factor of whooping cough. Despite global vaccination programs, this disease remains endemic in many countries and is still recognized as a significant public health problem. It is estimated that in 20…
View article: Genetic diversity of Francisella tularensis in Poland with comments on MLVA genotyping and a proposition of a novel rapid v4-genotyping
Genetic diversity of Francisella tularensis in Poland with comments on MLVA genotyping and a proposition of a novel rapid v4-genotyping Open
We investigated the genotypes of Francisella tularensis (F. tularensis) strains isolated in Poland during the period 1953-2013 and studied their genetic relationship to F. tularensis strains isolated in other countries using MLVA. We exami…