Alena N. Joignant
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View article: Metabolite fingerprinting by infrared matrix-assisted laser desorption electrospray ionization mass spectrometry
Metabolite fingerprinting by infrared matrix-assisted laser desorption electrospray ionization mass spectrometry Open
The adoption of mass spectrometry for high-throughput screening in drug discovery has become increasingly prevalent and has enabled label-free screening against diverse targets. Cellular assays for phenotypic screening, however, are primar…
View article: Feasibility of IR‐MALDESI Mass Spectrometry Imaging of PFAS
Feasibility of IR‐MALDESI Mass Spectrometry Imaging of PFAS Open
Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are a class of emerging contaminants that have been in use industrially since the 1940s. Their long‐term and extensive commercial use has led to their ubiquitous presence in the environm…
View article: Metabolite Fingerprinting for Phenotypic Screening by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry
Metabolite Fingerprinting for Phenotypic Screening by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Open
The adoption of mass spectrometry for high-throughput screening in drug discovery has become increasingly prevalent and has enabled label-free screening against diverse targets. Cellular assays for phenotypic screening, however, are primar…
View article: Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix‐assisted laser desorption electrospray ionization
Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix‐assisted laser desorption electrospray ionization Open
Rationale Infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) utilizes a 2970 nm mid‐IR laser to desorb samples with depth resolutions ( Z ) on the order of micrometers. Conventionally, 5–20 μm thick tissue secti…
View article: Maximized Spatial Information and Minimized Acquisition Time of Top-Hat IR-MALDESI-MSI of Zebrafish Using Nested Regions of Interest (nROIs)
Maximized Spatial Information and Minimized Acquisition Time of Top-Hat IR-MALDESI-MSI of Zebrafish Using Nested Regions of Interest (nROIs) Open
Increasing the spatial resolution of a mass spectrometry imaging (MSI) method results in a more defined heatmap of the spatial distribution of molecules across a sample, but it is also associated with the disadvantage of increased acquisit…
View article: Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non‐flat samples By Alena N. Joignant, Ying Xi and David C. Muddiman
Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non‐flat samples By Alena N. Joignant, Ying Xi and David C. Muddiman Open
This month’s tutorial walks through the parameters that affect the laser ablation-based mass spectrometry imaging (MSI) of non-flat surfaces with the ambition of making MSI-relevant considerations more accessible to the broader community. …
View article: Time of acquisition and high spatial resolution mass spectrometry imaging By Mary F. Wang, Alena N. Joignant, Alexandria L. Sohn, Kenneth P. Garrard and David C. Muddiman
Time of acquisition and high spatial resolution mass spectrometry imaging By Mary F. Wang, Alena N. Joignant, Alexandria L. Sohn, Kenneth P. Garrard and David C. Muddiman Open
This month's tutorial relates time of acquisition and high spatial resolution in mass spectrometry imaging (MSI) applications. The tutorial addresses many of the challenges presented by high spatial resolution MSI and resulting data file s…
View article: Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non‐flat samples
Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non‐flat samples Open
Biospecimens with nearly flat surfaces on a flat stage are typically required for laser‐based mass spectrometry imaging (MSI) techniques. However, sampling stages are rarely perfectly level, and accounting for this and the need to accommod…
View article: Time of acquisition and high spatial resolution mass spectrometry imaging
Time of acquisition and high spatial resolution mass spectrometry imaging Open
The field of mass spectrometry imaging (MSI) is constantly evolving to analyze a diverse array of biological systems. A common goal is the need to resolve cellular and subcellular heterogeneity with high spatial resolution. As the field co…
View article: SMART: A data reporting standard for mass spectrometry imaging
SMART: A data reporting standard for mass spectrometry imaging Open
Mass spectrometry imaging (MSI) is an important analytical technique that simultaneously reports the spatial location and abundance of detected ions in biological, chemical, clinical, and pharmaceutical studies. As MSI grows in popularity,…
View article: Improved spatial resolution of infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging using a reflective objective
Improved spatial resolution of infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging using a reflective objective Open
Rationale The level of visual detail of a mass spectrometry image is dependent on the spatial resolution with which it is acquired, which is largely determined by the focal diameter in infrared laser ablation‐based techniques. While the us…
View article: Developing transmission mode for infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging
Developing transmission mode for infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging Open
Rationale The development and characterization of the novel NextGen infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) source catalyzed new advancements in IR‐MALDESI instrumentation, including the development o…