Alessandro Mussida
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View article: Affinity-Based Copolymer Coating for Oriented Protein Immobilization in Biosensor Development
Affinity-Based Copolymer Coating for Oriented Protein Immobilization in Biosensor Development Open
Effective protein immobilization is a critical step in biosensor development, as it ensures the stability, functionality, and orientation of biomolecules on the sensor surface. Here, we present a novel affinity-based terpolymer coating des…
View article: Affinity-based copolymer coating for oriented protein immo-bilization in biosensor development
Affinity-based copolymer coating for oriented protein immo-bilization in biosensor development Open
Effective protein immobilization is a critical step in biosensor development, as it ensures the stability, functionality, and orientation of biomolecules on the sensor surface. Here, we present a novel affinity-based terpolymer coating des…
View article: Affinity-based copolymer coating for oriented protein immo-bilization in biosensor development
Affinity-based copolymer coating for oriented protein immo-bilization in biosensor development Open
Effective protein immobilization is a critical step in biosensor development, as it ensures the stability, functionality, and orientation of biomolecules on the sensor surface. Here, we present a novel affinity-based terpolymer coating des…
View article: PET Foils Functionalized with Reactive Copolymers as Adaptable Microvolume ELISA Spot Array Platforms for Multiplex Serological Analysis of SARS-CoV-2 Infections
PET Foils Functionalized with Reactive Copolymers as Adaptable Microvolume ELISA Spot Array Platforms for Multiplex Serological Analysis of SARS-CoV-2 Infections Open
Microvolume ELISA platforms have become vital in diagnostics for their high-throughput capabilities and minimal sample requirements. High-quality substrates with advanced surface properties are essential for these applications. They enable…
View article: Copolymer-Coated Gold Nanoparticles: Enhanced Stability and Customizable Functionalization for Biological Assays
Copolymer-Coated Gold Nanoparticles: Enhanced Stability and Customizable Functionalization for Biological Assays Open
Gold nanoparticles (AuNPs) play a vital role in biotechnology, medicine, and diagnostics due to their unique optical properties. Their conjugation with antibodies, antigens, proteins, or nucleic acids enables precise targeting and enhances…
View article: Copolymer-Coated Gold Nanoparticles: Enhanced Stability and Customizable Functionalization for Biological Assays
Copolymer-Coated Gold Nanoparticles: Enhanced Stability and Customizable Functionalization for Biological Assays Open
Gold nanoparticles (AuNPs) play a vital role in biotechnology, medicine, and diagnostics due to their unique optical properties. Their conjugation with antibodies, antigens, proteins, or nucleic acids enables precise targeting and enhances…
View article: Immobilization of biotinylated antibodies through streptavidin binding aptamer
Immobilization of biotinylated antibodies through streptavidin binding aptamer Open
Liquid biopsy approaches are powerful strategies that potentially allow the diagnosis and prognosis of a number of diseases. The field is continuously and rapidly growing, encouraging the discovery of novel predictory biomarkers. Antibodie…
View article: Immobilization of biotinylated antibodies through streptavidin binding aptamer
Immobilization of biotinylated antibodies through streptavidin binding aptamer Open
Liquid biopsy approaches are powerful strategies that potentially allow the diagnosis and prognosis of a number of diseases. The field is continuously and rapidly growing, encouraging the discovery of novel predictory biomarkers. Antibodie…
View article: Immobilization of biotinylated antibodies through streptavidin binding aptamer
Immobilization of biotinylated antibodies through streptavidin binding aptamer Open
Liquid biopsy approaches are powerful strategies that potentially allow the diagnosis and prognosis of a number of diseases. The field is continuously and rapidly growing, encouraging the discovery of novel predictory biomarkers. Antibodie…
View article: Optimization of Functional Group Concentration of N, N-Dimethylacrylamide-based Polymeric Coatings and Probe Immobilization for DNA and Protein Microarray Applications
Optimization of Functional Group Concentration of N, N-Dimethylacrylamide-based Polymeric Coatings and Probe Immobilization for DNA and Protein Microarray Applications Open
We report here a deep investigation into the effect of the concentration of a polymeric coating’s functional groups on probe density immobilization with the aim of establishing the optimal formulation to be implemented in specific microarr…
View article: Multifunctional membranes for lipidic nanovesicle capture
Multifunctional membranes for lipidic nanovesicle capture Open
Tangential flow filtration membrane systems are employed for the isolation and concentration of extracellular vesicles. However, interfacial interactions between the membrane surface and species influence the flux and membrane performance.…
View article: Polymeric Coating of Silica Microspheres for Biological Applications: Suppression of Non-Specific Binding and Functionalization with Biomolecules
Polymeric Coating of Silica Microspheres for Biological Applications: Suppression of Non-Specific Binding and Functionalization with Biomolecules Open
The use of micro- and nanoparticles in biological applications has dramatically grown during the last few decades due to the ease of protocols development and compatibility with microfluidics devices. Particles can be composed by different…
View article: Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application Open
The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been ob…
View article: SARS-CoV-2 Epitope Mapping on Microarrays Highlights Strong Immune-Response to N Protein Region
SARS-CoV-2 Epitope Mapping on Microarrays Highlights Strong Immune-Response to N Protein Region Open
A workflow for rapid SARS-CoV-2 epitope discovery on peptide microarrays is herein reported. The process started with a proteome-wide screening of immunoreactivity based on the use of a high-density microarray followed by a refinement and …
View article: SARS-CoV-2 epitope mapping on microarrays highlights strong immune-response to N protein region
SARS-CoV-2 epitope mapping on microarrays highlights strong immune-response to N protein region Open
A workflow for SARS-CoV-2 epitope discovery on peptide microarrays is herein reported. The process started with a proteome-wide screening of immunoreactivity based on the use of a high-density microarray followed by a refinement and valida…
View article: A Bi-Functional Polymeric Coating for the Co-Imobilization of Proteins and Peptides on Microarray Substrates
A Bi-Functional Polymeric Coating for the Co-Imobilization of Proteins and Peptides on Microarray Substrates Open
The analytical performance of the microarray technique in screening the affinity and reactivity of several probes towards a specific target, is highly affected by the coupling chemistry adopted to bind probes to the surface. However, the s…
View article: A Bi-Functional Polymeric Coating for the Co-Imobilization of Proteins and Peptides on Microarray Substrates
A Bi-Functional Polymeric Coating for the Co-Imobilization of Proteins and Peptides on Microarray Substrates Open
The analytical performance of the microarray technique in screening the affinity and reactivity of several probes towards a specific target, is highly affected by the coupling chemistry adopted to bind probes to the surface. However, the s…