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View article: DNA immunisation. New histochemical and morphometric data
DNA immunisation. New histochemical and morphometric data Open
Splenic germinal center reactions were measured during primary response to a plasmidic DNA intramuscular injection. Cardiotoxin-pretreated Balb/c mice were immunized with DNA plasmids encodmg or not the SAG1 protein, a membrane antigen of …
View article: Recombinant gp350 Vaccine for Infectious Mononucleosis: A Phase 2, Randomized, Double‐Blind, Placebo‐Controlled Trial to Evaluate the Safety, Immunogenicity, and Efficacy of an Epstein‐Barr Virus Vaccine in Healthy Young Adults
Recombinant gp350 Vaccine for Infectious Mononucleosis: A Phase 2, Randomized, Double‐Blind, Placebo‐Controlled Trial to Evaluate the Safety, Immunogenicity, and Efficacy of an Epstein‐Barr Virus Vaccine in Healthy Young Adults Open
ClinicalTrials.gov identifier: NCT00430534.
View article: PCR-Reverse Line Blot Typing Method Underscores the Genomic Heterogeneity of <i>Borrelia valaisiana</i> Species and Suggests Its Potential Involvement in Lyme Disease
PCR-Reverse Line Blot Typing Method Underscores the Genomic Heterogeneity of <i>Borrelia valaisiana</i> Species and Suggests Its Potential Involvement in Lyme Disease Open
Detection of the Borrelia burgdorferi sensu lato complex in biological samples is currently done by conventional immunological and molecular biological methods. To improve on the accuracy of these methods and to simplify the procedure for …
View article: Reverse transcription-polymerase chain reaction construction of plasmid-based, full-length cDNA libraries from Leishmania infantum for in vitro expression screening
Reverse transcription-polymerase chain reaction construction of plasmid-based, full-length cDNA libraries from Leishmania infantum for in vitro expression screening Open
We describe a streamlined reverse transcription-polymerase chain reaction methodology for constructing full-length cDNA libraries of trypanosomatids on the basis of conserved sequences located at the 5' and 3'ends of trans-spliced mRNAs. T…
View article: Eubacterial HslV and HslU Subunits Homologs in Primordial Eukaryotes
Eubacterial HslV and HslU Subunits Homologs in Primordial Eukaryotes Open
ATP-dependent protease complexes are present in all three kingdoms of life, where they rid the cell of misfolded or damaged proteins and control the level of certain regulatory proteins. They include the proteasome in Eukaryotes, Archea, a…
View article: Characterization of a Novel Salivary Immunosuppressive Protein from Ixodes ricinus Ticks
Characterization of a Novel Salivary Immunosuppressive Protein from Ixodes ricinus Ticks Open
In tick salivary glands, several genes are induced during the feeding process, leading to the expression of new proteins. These proteins are typically secreted in tick saliva and are potentially involved in the modulation of the host immun…
View article: Isolation of Ixodes ricinus salivary gland mRNA encoding factors induced during blood feeding.
Isolation of Ixodes ricinus salivary gland mRNA encoding factors induced during blood feeding. Open
In tick salivary glands, genes induced during blood feeding result in the expression of new proteins secreted into tick saliva. These proteins are potentially involved in modulation of vertebrate host immune and hemostatic responses. In th…
View article: NCX1 Na/Ca exchanger splice variants in pancreatic islet cells
NCX1 Na/Ca exchanger splice variants in pancreatic islet cells Open
In the rat pancreatic beta-cell, Na/Ca exchange displays a quite high capacity. The cell is equipped with two alternatively spliced Na/Ca exchanger-1 (NCX1) isoforms, namely NCX1.3 and NCX1.7. To examine the existence of a possible functio…
View article: Protective Immunity against Congenital Toxoplasmosis with Recombinant SAG1 Protein in a Guinea Pig Model
Protective Immunity against Congenital Toxoplasmosis with Recombinant SAG1 Protein in a Guinea Pig Model Open
Primary infection with Toxoplasma gondii during pregnancy can induce fetal pathology and abortion in both humans and animals. The present study describes the development of an experimental model of congenital toxoplasmosis in the guinea pi…
View article: A Human Milk Factor Susceptible to Cathepsin D Inhibitors Enhances Human Immunodeficiency Virus Type 1 Infectivity and Allows Virus Entry into a Mammary Epithelial Cell Line
A Human Milk Factor Susceptible to Cathepsin D Inhibitors Enhances Human Immunodeficiency Virus Type 1 Infectivity and Allows Virus Entry into a Mammary Epithelial Cell Line Open
Human immunodeficiency virus type 1 (HIV-1) growth in lymphocyte cultures was increased when the virus inoculum was incubated in breast milk. The enhancing effect of milk was abolished by anti-cathepsin D antibody or by pepstatin A, a cath…
View article: The Sulfonium Ion Linkage in Myeloperoxidase
The Sulfonium Ion Linkage in Myeloperoxidase Open
The heme group of myeloperoxidase is covalently linked via two ester bonds to the protein and a unique sulfonium ion linkage involving Met(243). Mutation of Met(243) into Thr, Gln, and Val, which are the corresponding residues of eosinophi…
View article: Characterization of the Asp94 and Glu242 mutants in myeloperoxidase, the residues linking the heme group via ester bonds
Characterization of the Asp94 and Glu242 mutants in myeloperoxidase, the residues linking the heme group via ester bonds Open
The heme group of all mammalian peroxidases is covalently linked to the protein matrix via two esterbonds, as we have recently shown by Fourier transform infrared (FTIR) difference spectroscopy [Kooter, I.M., Pierik, A.J., Merkx, M., Averi…
View article: Contribution of the second transmembrane helix of the secretin receptor to the positioning of secretin
Contribution of the second transmembrane helix of the secretin receptor to the positioning of secretin Open
The secretin amino‐terminal residues are essential for high affinity binding to its cognate receptor and for its biological activity. Mutation of the [Asp 3 ] residue of secretin to [Asn 3 ] decreased the ligand's affinity for the rat wild…
View article: Enhancement of macrophage‐mediated bactericidal activity by macrophage‐mannose receptor‐ligand interaction
Enhancement of macrophage‐mediated bactericidal activity by macrophage‐mannose receptor‐ligand interaction Open
Neutrophils represent one of the host's primary defenses against invading organisms. These cells often arrive at the site of infection prior to macrophages (MØ). Neutrophils release myeloperoxidase (MPO) into the micro‐environment during p…
View article: Epitopes recognized by human T lymphocytes in the ROP2 protein antigen of Toxoplasma gondii
Epitopes recognized by human T lymphocytes in the ROP2 protein antigen of Toxoplasma gondii Open
The ROP2 protein of Toxoplasma gondii possesses immunological and biological properties which have led to its proposal as a vaccine candidate. To identify epitopes recognized by human T cells in the ROP2 antigen, we submitted the sequence …
View article: Interaction of Amino Acid Residues at Positions 8–15 of Secretin with the N‐Terminal Domain of the Secretin Receptor
Interaction of Amino Acid Residues at Positions 8–15 of Secretin with the N‐Terminal Domain of the Secretin Receptor Open
The ability of secretin, PACAP‐(1–27)‐peptide, and ten hybrid peptides to recognize and activate the rat secretin and vasoactive intestinal polypeptide (PACAP type II VIP 1 ) receptors was tested on recombinant Chinese hamster ovary (CHO) …
View article: Colorimetric Solid-Phase Capture Hybridization Assay for Detection of Amplified <i>Borrelia burgdorferi</i> DNA
Colorimetric Solid-Phase Capture Hybridization Assay for Detection of Amplified <i>Borrelia burgdorferi</i> DNA Open
To simplify detection procedures of DNA fragments resulting from PCR, we developed a colorimetric microplate hybridization assay. This format was used for the identification of Borrelia burgdorferi sensu lato, the causal agent of Lyme dise…
View article: Phagocytosis and Intracellular Killing of Candida albicans by Macrophages Exposed to Myeloperoxidase
Phagocytosis and Intracellular Killing of Candida albicans by Macrophages Exposed to Myeloperoxidase Open
Candida albicans is an opportunistic pathogen whose resurgence coincides with the rising number of AIDS patients. Neutrophils are known to be involved in the clearance of Candida infections; however, the role of macrophages in host defense…
View article: Rapid colorimetric hybridization assay for detecting amplified Helicobacter pylori DNA in gastric biopsy specimens
Rapid colorimetric hybridization assay for detecting amplified Helicobacter pylori DNA in gastric biopsy specimens Open
A very simple, practical, sensitive, and specific colorimetric hybridization assay for detecting amplified Helicobacter pylori DNA is described. This assay, which combines a sensitive sandwich DNA hybridization reaction and a colorimetric …
View article: Neutrophil and recombinant myeloperoxidase as antigens in ANCA positive systemic vasculitis
Neutrophil and recombinant myeloperoxidase as antigens in ANCA positive systemic vasculitis Open
SUMMARY Myeloperoxidase (MPO) is one of the major autoantigens recognized by anti-neutrophil cytoplasm antibodies. The association of this antigen with specific disease entities requires that there is a source of pure antigen present in la…
View article: Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens
Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens Open
A PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with specific primers for ureC gene amplification (herein referred to as ureC PCR) was compared with other routine invasive methods (culture, the rapid-urease…
View article: Simultaneous Infection of <i>Ixodes ricinus</i> Nymphs by Two <i>Borrelia burgdorferi</i> Sensu Lato Species: Possible Implications for Clinical Manifestations
Simultaneous Infection of <i>Ixodes ricinus</i> Nymphs by Two <i>Borrelia burgdorferi</i> Sensu Lato Species: Possible Implications for Clinical Manifestations Open
info:eu-repo/semantics/published
View article: Exogenous myeloperoxidase enhances bacterial phagocytosis and intracellular killing by macrophages
Exogenous myeloperoxidase enhances bacterial phagocytosis and intracellular killing by macrophages Open
It is well documented that myeloperoxidase (MyPo) contributes to the bacterial activities of neutrophils and monocytes. Since mature macrophages (M phi) are devoid of this enzyme, its participation in M phi-mediated phagocytes and bacteria…
View article: Heme-Protein Interaction in Myeloperoxidase: Modification of Spectroscopic Properties and Catalytic Activity by Single Residue Mutation
Heme-Protein Interaction in Myeloperoxidase: Modification of Spectroscopic Properties and Catalytic Activity by Single Residue Mutation Open
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHeme-Protein Interaction in Myeloperoxidase: Modification of Spectroscopic Properties and Catalytic Activity by Single Residue MutationRene Floris, Nicole Moguilevsky, Gerwin Puppels, Alain Jacqu…
View article: Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients
Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients Open
Oligonucleotide primers based on Borrelia burgdorferi sensu lato ospA gene sequences have been designed for use in the PCR to type all (SL primers) or each (GI to GIII primers) of the B. burgdorferi sensu lato genospecies involved in Lyme …
View article: Pharmacological and Functional Characterisation of the Wild—Type and Site—Directed Mutants of the Human H<sub>1</sub>Histamine Receptor Stably Expressed in CHO Cells
Pharmacological and Functional Characterisation of the Wild—Type and Site—Directed Mutants of the Human H<sub>1</sub>Histamine Receptor Stably Expressed in CHO Cells Open
A cDNA clone for the human histamine H1 receptor was isolated from a lung cDNA library and stably expressed in CHO cells. The recombinant receptor protein present in the cell membranes, displayed the functional and binding characteristics …
View article: Stable Expression of Human H<sub>1</sub>‐histamine‐receptor cDNA in Chinese Hamster Ovary Cells
Stable Expression of Human H<sub>1</sub>‐histamine‐receptor cDNA in Chinese Hamster Ovary Cells Open
A cDNA clone for the histamine H 1 receptor was isolated from a human lung cDNA library; it encoded a protein of 487 amino acids which showed characteristic features of G‐protein‐coupled receptors. The percentages of identity of the deduce…
View article: Intracellular retention and degradation of human mutant variant of a α1‐antitrypsin in stably transfected Chinese hamster ovary cell lines
Intracellular retention and degradation of human mutant variant of a α1‐antitrypsin in stably transfected Chinese hamster ovary cell lines Open
Normal (PiM) and mutant (PiZ) variants of human α 1 ‐antitrypsin (α 1 ‐AT) cDNA, cloned into the pTnd eucaryotic expression vector, were used to derive recombinant Chinese hamster ovary cell lines permanently expressing the corresponding p…
View article: Candidicidal activity of the human recombinant myeloperoxidase system
Candidicidal activity of the human recombinant myeloperoxidase system Open
The effect of the human recombinant myeloperoxidase (recMPO) system on the viability of Candida albicans blastospores was determined. Cells were incubated in the presence of the enzyme, H2O2, and various substrates. In optimal conditions, …