Alex Chenchik
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View article: Streamlined 96-well plate method for simultaneous single-cell TCR immune receptor repertoire analysis and immunophenotyping
Streamlined 96-well plate method for simultaneous single-cell TCR immune receptor repertoire analysis and immunophenotyping Open
This study describes an efficient immune profiling method by FACS-sorting single cells in a 96-well plate to assess clonotype repertoire diversity, paired-chain information, and cell phenotypes in a single, multiplex RT-PCR-based assay wit…
View article: 154 Universal synthetic spike-in controls for accurate adaptive immune receptor profiling
154 Universal synthetic spike-in controls for accurate adaptive immune receptor profiling Open
Background The results of adaptive immune receptor (AIR) repertoire diversity assays can be affected by various biases from differences in conditions in the RT-PCR and NGS sequencing steps. Spike-in synthetic controls can be used as calibr…
View article: 165 Microsampling for RNA biomarker profiling in blood
165 Microsampling for RNA biomarker profiling in blood Open
Background Multi-omic analysis of microsamples of lancet-induced blood drops allows frequent capture and quantitation of numerous metabolites, lipids, cytokines, and proteins. Microsample-based transcriptome profiling would facilitate use …
View article: T-cell and B-cell receptor repertoire profiling for biomarker discovery
T-cell and B-cell receptor repertoire profiling for biomarker discovery Open
T-cell receptor (TCR) and B-cell receptor (BCR) repertoire profiling, also referred to as adaptive immune receptor repertoire (AIRR) profiling, holds great potential for the understanding of disease mechanisms and for the development of ne…
View article: The 10th Santorini conference: Systems medicine, personalised health and therapy. “The odyssey from hope to practice: Patient first. Keep Ithaca always in your mind”, Santorini, Greece, 23–26 May 2022
The 10th Santorini conference: Systems medicine, personalised health and therapy. “The odyssey from hope to practice: Patient first. Keep Ithaca always in your mind”, Santorini, Greece, 23–26 May 2022 Open
International audience
View article: 3 Adaptive immune receptor repertoire profiling for biomarker discovery
3 Adaptive immune receptor repertoire profiling for biomarker discovery Open
Background Adaptive Immune Receptor (AIR) Repertoire profiling and characterization of antigen-activated immune cells is essential for the discovery of novel prognostic and predictive biomarkers and for studying immune response mechanisms …
View article: Oncogenic context shapes the fitness landscape of tumor suppression
Oncogenic context shapes the fitness landscape of tumor suppression Open
Tumors acquire alterations in oncogenes and tumor suppressor genes in an adaptive walk through the fitness landscape of tumorigenesis. However, the features of this landscape remain poorly understood and cannot be revealed by human cancer …
View article: 69 Immunophenotyping of TCR and BCR clonotypes
69 Immunophenotyping of TCR and BCR clonotypes Open
Background T-cell receptor (TCR) and B-cell receptor (BCR) repertoire profiling holds great potential for understanding disease mechanisms and for the development of new therapeutics in infectious diseases, autoimmunity and in immuno-oncol…
View article: Cancer immunenet biomarker profiling panel
Cancer immunenet biomarker profiling panel Open
Meeting abstracts Several recent studies have now firmly established a link between the immune infiltrate in several human carcinoma types and prognosis and/or response to therapy. Correspondingly, increasing evidence suggests that the num…
View article: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance Open
Key Points A function-first shRNA library screen identifies pathways involved in BCR-ABL1 kinase-independent TKI resistance. RAN or XPO1 inhibition impairs survival of progenitors from newly diagnosed or TKI-resistant CML patients.