Alexander I. Taylor
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View article: Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes
Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes Open
Nucleic acid catalysts can carry over into cellular RNA preparations following transfection, causing false positive and false negative knockdown measurements.
View article: Characterization of an HNA aptamer suggests a non-canonical G-quadruplex motif
Characterization of an HNA aptamer suggests a non-canonical G-quadruplex motif Open
Nucleic acids not only form the basis of heredity, but are increasingly a source of novel nano-structures, -devices and drugs. This has spurred the development of chemically modified alternatives (xeno nucleic acids (XNAs)) comprising chem…
View article: XNAzymes targeting the SARS-CoV-2 genome inhibit viral infection
XNAzymes targeting the SARS-CoV-2 genome inhibit viral infection Open
View article: A two-residue nascent-strand steric gate controls synthesis of 2′-O-methyl- and 2′-O-(2-methoxyethyl)-RNA
A two-residue nascent-strand steric gate controls synthesis of 2′-O-methyl- and 2′-O-(2-methoxyethyl)-RNA Open
View article: Targeting non-coding RNA family members with artificial endonuclease XNAzymes
Targeting non-coding RNA family members with artificial endonuclease XNAzymes Open
View article: A modular XNAzyme cleaves long, structured RNAs under physiological conditions and enables allele-specific gene silencing
A modular XNAzyme cleaves long, structured RNAs under physiological conditions and enables allele-specific gene silencing Open
View article: Efficient synthesis and replication of diverse sequence libraries composed of biostable nucleic acid analogues
Efficient synthesis and replication of diverse sequence libraries composed of biostable nucleic acid analogues Open
Blends of engineered polymerases enable efficient DNA-templated synthesis and reverse transcription of diverse-sequence oligonucleotide libraries composed of locked nucleic acid (LNA), 2′- O -methyl-RNA (2′OMe-RNA), or mixtures of the two.
View article: Catalysts from synthetic genetic polymers
Catalysts from synthetic genetic polymers Open
The emergence of catalysis in early genetic polymers like RNA is considered a key transition in the origin of life1, predating the appearance of protein enzymes. DNA also demonstrates the capacity to fold into three-dimensional structures …
View article: Discovery and evolution of RNA and XNA reverse transcriptase function and fidelity
Discovery and evolution of RNA and XNA reverse transcriptase function and fidelity Open
View article: Beyond DNA and RNA: The Expanding Toolbox of Synthetic Genetics
Beyond DNA and RNA: The Expanding Toolbox of Synthetic Genetics Open
The remarkable physicochemical properties of the natural nucleic acids, DNA and RNA, define modern biology at the molecular level and are widely believed to have been central to life's origins. However, their ability to form repositories o…
View article: Random-sequence genetic oligomer pools display an innate potential for ligation and recombination
Random-sequence genetic oligomer pools display an innate potential for ligation and recombination Open
Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes. Here, we des…
View article: Author response: Random-sequence genetic oligomer pools display an innate potential for ligation and recombination
Author response: Random-sequence genetic oligomer pools display an innate potential for ligation and recombination Open
View article: Innate potential of random genetic oligomer pools for recombination
Innate potential of random genetic oligomer pools for recombination Open
The spontaneous emergence of function from prebiotic pools of informational polymers is a central conjecture of current origin of life scenarios. However, the innate functional capacity of random genetic polymer pools is unknown. Here, we …
View article: Nanostructures from Synthetic Genetic Polymers
Nanostructures from Synthetic Genetic Polymers Open
Nanoscale objects of increasing complexity can be constructed from DNA or RNA. However, the scope of potential applications could be enhanced by expanding beyond the moderate chemical diversity of natural nucleic acids. Here, we explore th…
View article: Enzymatic Synthesis of Nucleic Acids with Defined Regioisomeric 2′‐5′ Linkages
Enzymatic Synthesis of Nucleic Acids with Defined Regioisomeric 2′‐5′ Linkages Open
Information‐bearing nucleic acids display universal 3′‐5′ linkages, but regioisomeric 2′‐5′ linkages occur sporadically in non‐enzymatic RNA synthesis and may have aided prebiotic RNA replication. Herein we report on the enzymatic synthesi…
View article: Enzymatische Synthese von Nukleinsäuren mit definierten regioisomeren 2′‐5′‐Verknüpfungen
Enzymatische Synthese von Nukleinsäuren mit definierten regioisomeren 2′‐5′‐Verknüpfungen Open
Informationentragende Nukleinsäuren sind universell über 3′‐5′‐Bindungen verknüpft. Regioisomere 2′‐5′‐Verknüpfungen bilden sich dagegen unregelmäßig während der nichtenzymatischen RNA‐Synthese und waren womöglich hilfreich für die präbiot…