Alexander J. Bradshaw
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View article: Whole genome sequencing of historical specimens from the world's largest fungal collection yields high‐quality assemblies
Whole genome sequencing of historical specimens from the world's largest fungal collection yields high‐quality assemblies Open
Summary High‐throughput molecular studies of museum specimens (museomics) have great potential in biodiversity research, but fungal historical collections have scarcely been examined, leading to no comprehensive methodological assessments.…
View article: Characterization of a core fungal community and captivity-induced “mycobiome” change in Fowler’s Toad (<i>Anaxyrus fowleri</i>)
Characterization of a core fungal community and captivity-induced “mycobiome” change in Fowler’s Toad (<i>Anaxyrus fowleri</i>) Open
Amphibious animals, such as frogs, are found at the intersection of aquatic and terrestrial ecosystems. They often serve as keystone and sentinel species, essential in nutrient cycling and food webs. In recent decades, amphibians have expe…
View article: Generative hyphal stiffness and cell wall thickening in fungi
Generative hyphal stiffness and cell wall thickening in fungi Open
Filamentous fungi produce multinucleate, interconnected tubular structures called hyphae which are the building blocks of all fungal structures, including mushrooms and mycelium. This makes hyphae foundational to understanding all fungal m…
View article: Unraveling the Genomic and Phylogenetic Complexity of the understudied microfungus <i>Basidiobolus:</i> Insights from 19 Newly Sequenced Genomes
Unraveling the Genomic and Phylogenetic Complexity of the understudied microfungus <i>Basidiobolus:</i> Insights from 19 Newly Sequenced Genomes Open
Basidiobolus is a globally distributed genus of early-diverging fungi within Zoopagomycota, known for its presence in diverse ecological niches ranging from soil and decaying organic matter to vertebrate gastrointestinal tracts. Despite it…
View article: Effects of urbanization on the microbial fungal communities from water, sediment, and amphibian hosts across a rural-to-urban waterway in Worcester, Massachusetts
Effects of urbanization on the microbial fungal communities from water, sediment, and amphibian hosts across a rural-to-urban waterway in Worcester, Massachusetts Open
In a world of increasingly urbanized environments, it is critical to understand the impact of urbanization on microbial communities, including fungal communities, as a measure of ecosystem health, and to document how these environments are…
View article: Phylogenomic insights into the taxonomy, ecology, and mating systems of the lorchel family Discinaceae (Pezizales, Ascomycota)
Phylogenomic insights into the taxonomy, ecology, and mating systems of the lorchel family Discinaceae (Pezizales, Ascomycota) Open
Lorchels, also known as false morels (Gyromitra sensu lato), are iconic due to their brain-shaped mushrooms and production of gyromitrin, a deadly mycotoxin. Molecular phylogenetic studies have hitherto failed to resolve deep-branching rel…
View article: Discovery of the closest free-living relative of the domesticated “magic mushroom”<i>Psilocybe cubensis</i>in Africa
Discovery of the closest free-living relative of the domesticated “magic mushroom”<i>Psilocybe cubensis</i>in Africa Open
The “magic mushroom” Psilocybe cubensis is cultivated worldwide for recreational and medicinal uses. Described initially from Cuba in 1904, there has been substantial debate about its origin and diversification. The prevailing view, first …
View article: Fungal cryopreservation across 61 genera: Practical application and method evaluation
Fungal cryopreservation across 61 genera: Practical application and method evaluation Open
Fungi occupy important environmental, cultural, and socioeconomic roles. However, biological research of this diverse kingdom has lagged behind that of other phylogenetic groups. This is partially the result of the notorious difficulty in …
View article: Phylogenomics of the psychoactive mushroom genus <i>Psilocybe</i> and evolution of the psilocybin biosynthetic gene cluster
Phylogenomics of the psychoactive mushroom genus <i>Psilocybe</i> and evolution of the psilocybin biosynthetic gene cluster Open
Psychoactive mushrooms in the genus Psilocybe have immense cultural value and have been used for centuries in Mesoamerica. Despite the recent surge of interest in these mushrooms due to the psychotherapeutic potential of their natural alka…
View article: Phylogenomics, biogeography, and trait evolution of the Boletaceae (Boletales, Agaricomycetes, Basidiomycota)
Phylogenomics, biogeography, and trait evolution of the Boletaceae (Boletales, Agaricomycetes, Basidiomycota) Open
The species-rich porcini mushroom family Boletaceae is a widespread and well-known group of ectomycorrhizal (ECM) mushroom-forming fungi that has eluded intrafamilial phylogenetic resolution despite many attempts using morphological traits…
View article: From populations to pan-genomes: investigating the role of ecology and genomic architecture in maintaining species boundaries in the porcini mushroom,<i>Boletus edulis</i>
From populations to pan-genomes: investigating the role of ecology and genomic architecture in maintaining species boundaries in the porcini mushroom,<i>Boletus edulis</i> Open
Hybridization among diverged species is common across all domains of eukaryotic life, but appears to be particularly common in fungi. However, some fungi exhibit a greater tendency for hybridization than others and it is unclear what mecha…
View article: Effect of agar concentration on structure and physiology of fungal hyphal systems
Effect of agar concentration on structure and physiology of fungal hyphal systems Open
Hyphae are the filamentous branches that form a mycelial network, acting as the constitutive structure of mushroom-forming Fungi. Hyphae grow on many substrates and in multitudes of environmental conditions. Due to this versatility, hyphae…
View article: Phylogenomics of the psychoactive mushroom genus<i>Psilocybe</i>and evolution of the psilocybin biosynthetic gene cluster
Phylogenomics of the psychoactive mushroom genus<i>Psilocybe</i>and evolution of the psilocybin biosynthetic gene cluster Open
Psychoactive mushrooms in the genus Psilocybe have immense cultural value and have been used for centuries in Mesoamerica. Despite a recent surge in interest in these mushrooms due to emerging evidence that psilocybin, the main psychoactiv…
View article: DNA Authentication and Chemical Analysis of <i>Psilocybe</i> Mushrooms Reveal Widespread Misdeterminations in Fungaria and Inconsistencies in Metabolites
DNA Authentication and Chemical Analysis of <i>Psilocybe</i> Mushrooms Reveal Widespread Misdeterminations in Fungaria and Inconsistencies in Metabolites Open
The therapeutic use of psilocybin, the active ingredient in “magic mushrooms,” is revolutionizing mental health care for a number of conditions, including depression, posttraumatic stress disorder (PTSD), and end-of-life care. This has spo…
View article: Sigma GeneElute total RNA extraction from fungal tissue v1
Sigma GeneElute total RNA extraction from fungal tissue v1 Open
RNA extraction from fungal tissue using the Sigma GeneElute total RNA kit.
View article: On the origin of feces: Fungal diversity, distribution, and conservation implications from feces of small mammals
On the origin of feces: Fungal diversity, distribution, and conservation implications from feces of small mammals Open
Fungi are highly diverse, but only a small fraction of the total estimated species have been characterized. Often, the extent of diversity and distribution of fungal communities is difficult or near impossible to assess due to the fact tha…
View article: Genetic Analysis of the Salmonella FliE Protein That Forms the Base of the Flagellar Axial Structure
Genetic Analysis of the Salmonella FliE Protein That Forms the Base of the Flagellar Axial Structure Open
The FliE component of the bacterial flagellum is the first protein secreted through the flagellar type III secretion system (fT3SS) that is capable of self-assembly into the growing bacterial organelle. The FliE protein plays dual roles in…
View article: On the Origin of Feces: Fungal diversity, distribution, and conservation implications from feces of small mammals
On the Origin of Feces: Fungal diversity, distribution, and conservation implications from feces of small mammals Open
Fungi are extremely diverse, but only a small fraction of the total estimated species have been characterized. Often, the extent of diversity and distribution of fungal communities is difficult or near impossible to assess. This is due to …
View article: Peer Review #2 of "What’s for dinner this time?: DNA authentication of “wild mushrooms” in food products sold in the USA (v0.2)"
Peer Review #2 of "What’s for dinner this time?: DNA authentication of “wild mushrooms” in food products sold in the USA (v0.2)" Open
Mushrooms have been consumed by humans for thousands of years, and while some have gastronomic and nutritional value, it has long been recognized that only select species of mushrooms are suitable for consumption.Adverse health effects of …
View article: Peer Review #2 of "What’s for dinner this time?: DNA authentication of “wild mushrooms” in food products sold in the USA (v0.1)"
Peer Review #2 of "What’s for dinner this time?: DNA authentication of “wild mushrooms” in food products sold in the USA (v0.1)" Open
Mushrooms have been consumed by humans for thousands of years, and while some have gastronomic and nutritional value, it has long been recognized that only select species of mushrooms are suitable for consumption.Adverse health effects of …
View article: What’s for dinner this time?: DNA authentication of “wild mushrooms” in food products sold in the USA
What’s for dinner this time?: DNA authentication of “wild mushrooms” in food products sold in the USA Open
Mushrooms have been consumed by humans for thousands of years, and while some have gastronomic and nutritional value, it has long been recognized that only select species of mushrooms are suitable for consumption. Adverse health effects of…
View article: AxyPrep magnetic Bead normalization and PCR clean up For dual index v1
AxyPrep magnetic Bead normalization and PCR clean up For dual index v1 Open
In-house protocol used to prepare PCR amplicons with AxyPrep beads for Illumina sequencing.
View article: Convergent evolution of psilocybin biosynthesis by psychedelic mushrooms
Convergent evolution of psilocybin biosynthesis by psychedelic mushrooms Open
Psilocybin is a psychoactive compound with clinical applications produced by dozens of mushroom species 1 . There has been a longstanding interest in psilocybin research with regard to treatment for addiction 2 , depression 3 , and end-of-…