Alexander Ripp
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View article: The P(III)‐Amidite Based Synthesis of Stable Isotope Labeled mRNA‐Cap‐Structures Enables their Sensitive Quantitation from Brain Tissue
The P(III)‐Amidite Based Synthesis of Stable Isotope Labeled mRNA‐Cap‐Structures Enables their Sensitive Quantitation from Brain Tissue Open
The 5’ cap structure is crucial to mRNA function, with its diverse methylation patterns depending on the cellular state. Sensitive analytical methods are sought after to quantify this cap variety also referred to as cap epitranscriptome. T…
View article: The P(III)‐Amidite Based Synthesis of Stable Isotope Labeled mRNA‐Cap‐Structures Enables their Sensitive Quantitation from Brain Tissue
The P(III)‐Amidite Based Synthesis of Stable Isotope Labeled mRNA‐Cap‐Structures Enables their Sensitive Quantitation from Brain Tissue Open
The 5’ cap structure is crucial to mRNA function, with its diverse methylation patterns depending on the cellular state. Sensitive analytical methods are sought after to quantify this cap variety also referred to as cap epitranscriptome. T…
View article: Harnessing the Photoperformance of <i>N</i>‐Methyl‐Quinolinone for Gated Photo‐Driven Cyclability and Reversible Photoligation
Harnessing the Photoperformance of <i>N</i>‐Methyl‐Quinolinone for Gated Photo‐Driven Cyclability and Reversible Photoligation Open
[2π + 2π]‐photocycloadditions and their ability to trigger controlled and reversible photoligation through disparate wavelengths provide an attractive platform to unlock advanced functionalities in soft materials. Yet, among the limited am…
View article: PenTag, a Versatile Platform for Synthesizing Protein‐Polymer Biohybrid Materials
PenTag, a Versatile Platform for Synthesizing Protein‐Polymer Biohybrid Materials Open
The site‐specific and covalent conjugation of proteins on solid supports and in hydrogels is the basis for the synthesis of biohybrid materials offering broad applications. Current methods for conjugating proteins to desired targets are of…
View article: Sangivamycin is preferentially incorporated into viral RNA by the SARS-CoV-2 polymerase
Sangivamycin is preferentially incorporated into viral RNA by the SARS-CoV-2 polymerase Open
Sangivamycin (S) is an adenosine (A) nucleoside analog with low nanomolar antiviral activity against SARS-CoV-2 in vitro. Previously, low nanomolar antiviral efficacy was revealed when tested against multiple viral variants in several cell…
View article: PenTag, a Versatile Platform for Synthesizing Protein-Polymer Biohybrid Materials
PenTag, a Versatile Platform for Synthesizing Protein-Polymer Biohybrid Materials Open
The site-specific and covalent conjugation of proteins on solid supports and in hydrogels is the basis for the synthesis of biohybrid materials offering broad applications. Current methods for conjugating proteins to desired targets are of…
View article: CCDC 2062089: Experimental Crystal Structure Determination
CCDC 2062089: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Stable Isotope Phosphate Labelling of Diverse Metabolites is Enabled by a Family of <sup>18</sup>O‐Phosphoramidites**
Stable Isotope Phosphate Labelling of Diverse Metabolites is Enabled by a Family of <sup>18</sup>O‐Phosphoramidites** Open
Stable isotope labelling is state‐of‐the‐art in quantitative mass spectrometry, yet often accessing the required standards is cumbersome and very expensive. Here, a unifying synthetic concept for 18 O‐labelled phosphates is presented, base…
View article: Stable Isotope Phosphate Labelling of Diverse Metabolites is Enabled by a Family of <sup>18</sup> O‐Phosphoramidites**
Stable Isotope Phosphate Labelling of Diverse Metabolites is Enabled by a Family of <sup>18</sup> O‐Phosphoramidites** Open
Stable isotope labelling is state‐of‐the‐art in quantitative mass spectrometry, yet often accessing the required standards is cumbersome and very expensive. Here, a unifying synthetic concept for 18 O‐labelled phosphates is presented, base…
View article: The Aryne Phosphate Reaction**
The Aryne Phosphate Reaction** Open
Condensed phosphates are a critically important class of molecules in biochemistry. Non‐natural analogues are important for various applications, such as single‐molecule real‐time DNA sequencing. Often, such analogues contain more than thr…
View article: The Aryne Phosphate Reaction**
The Aryne Phosphate Reaction** Open
Condensed phosphates are a critically important class of molecules in biochemistry. Non‐natural analogues are important for various applications, such as single‐molecule real‐time DNA sequencing. Often, such analogues contain more than thr…
View article: The Aryne Phosphate Reaction
The Aryne Phosphate Reaction Open
Condensed phosphates are a critically important class of molecules in biochemistry, with a myriad of derived structures being known. Moreover, non-natural analogues are important for various applications, such as single molecule real time …
View article: The Aryne Phosphate Reaction
The Aryne Phosphate Reaction Open
Condensed phosphates are a critically important class of molecules in biochemistry, with a myriad of derived structures being known. Moreover, non-natural analogues are important for various applications, such as single molecule real time …
View article: Stable isotope phosphate labelling of diverse metabolites is enabled by a family of 18O-phosphoramidites
Stable isotope phosphate labelling of diverse metabolites is enabled by a family of 18O-phosphoramidites Open
Stable isotope labelling is state-of-the-art in quantitative mass spectrometry, yet often accessing the required standards is cumbersome and very expensive. As 18O can be derived from heavy water (H218O), it is comparably cheap and particu…
View article: Stable isotope phosphate labelling of diverse metabolites is enabled by a family of 18O-phosphoramidites
Stable isotope phosphate labelling of diverse metabolites is enabled by a family of 18O-phosphoramidites Open
Stable isotope labelling is state-of-the-art in quantitative mass spectrometry, yet often accessing the required standards is cumbersome and very expensive. As 18O can be derived from heavy water (H218O), it is comparably cheap and particu…
View article: Thiocoumarin Caged Nucleotides: Synthetic Access and Their Photophysical Properties
Thiocoumarin Caged Nucleotides: Synthetic Access and Their Photophysical Properties Open
Photocages have been successfully applied in cellular signaling studies for the controlled release of metabolites with high spatio-temporal resolution. Commonly, coumarin photocages are activated by UV light and the quantum yields of uncag…
View article: Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis Open
The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on …
View article: Rapid Synthesis of Nucleoside Triphosphates and Analogues
Rapid Synthesis of Nucleoside Triphosphates and Analogues Open
Nucleoside triphosphates (NTPs) are essential biomolecules involved in almost all biological processes, and their study is therefore critical to understanding cellular biology. Here, we describe a chemical synthesis suitable for obtaining …
View article: Synthesis of Modified Nucleoside Oligophosphates Simplified: Fast, Pure, and Protecting Group Free
Synthesis of Modified Nucleoside Oligophosphates Simplified: Fast, Pure, and Protecting Group Free Open
Phosphoramidite analogues of modified cyclotriphosphates provide a general and step-economical synthesis of nucleoside triphosphates and analogues on scale without the need for protecting groups. These reagents enable rapid access to pure …
View article: CCDC 1868137: Experimental Crystal Structure Determination
CCDC 1868137: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 1868136: Experimental Crystal Structure Determination
CCDC 1868136: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Investigations on <i>non-classical</i> silylium ions leading to a cyclobutenyl cation
Investigations on <i>non-classical</i> silylium ions leading to a cyclobutenyl cation Open
The formation of simple non-classical silylium ions from [Me3Si]+ sources and alkenes or alkynes was investigated, but mainly oligomerization was observed. Yet, the reaction with MeCCMe led to a room temperature stable cyclobutenyl cation…
View article: CCDC 1868138: Experimental Crystal Structure Determination
CCDC 1868138: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Using the Lewis Acid Me<sub>3</sub>Si−F−Al(OR<sup>F</sup>)<sub>3</sub> To Prepare Phosphino‐Phosphonium Cations with the Least‐Coordinating Anion [(R<sup>F</sup>O)<sub>3</sub>Al−F−Al(OR<sup>F</sup>)<sub>3</sub>]<sup>−</sup>
Using the Lewis Acid Me<sub>3</sub>Si−F−Al(OR<sup>F</sup>)<sub>3</sub> To Prepare Phosphino‐Phosphonium Cations with the Least‐Coordinating Anion [(R<sup>F</sup>O)<sub>3</sub>Al−F−Al(OR<sup>F</sup>)<sub>3</sub>]<sup>−</sup> Open
By reaction of two equivalents of Me 3 Si−F−Al(OR F ) 3 1 with an equimolar amount of PPh 2 Cl, the salt [Ph 2 P−PPh 2 Cl] + [(R F O) 3 Al−F−Al(OR F ) 3 ] − 2 is prepared smoothly in 91 % yield (NMR, XRD). The synthesis of [Ph 2 P−PPh 3 ] …
View article: CCDC 1522965: Experimental Crystal Structure Determination
CCDC 1522965: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …