Alexandra Schambony
YOU?
Author Swipe
View article: Cryo–light microscopy with angstrom precision deciphers structural conformations of PIEZO1 in its native state
Cryo–light microscopy with angstrom precision deciphers structural conformations of PIEZO1 in its native state Open
Investigations based on cryo–electron microscopy (cryo-EM), atomic force microscopy, and super-resolution microscopy reveal a symmetric trimer with propeller-like blades for the mechanosensitive ion channel PIEZO. However, a conclusive und…
View article: NCBP2-AS2 is a mitochondrial microprotein, regulates energy metabolism and neurogenesis, and is downregulated in Alzheimer’s disease
NCBP2-AS2 is a mitochondrial microprotein, regulates energy metabolism and neurogenesis, and is downregulated in Alzheimer’s disease Open
Microproteins, short functional peptides encoded by small genes, are emerging as critical regulators of cellular processes, yet their roles in mitochondrial function and neurodegeneration remain underexplored. In this study, we identify NC…
View article: Cryogenic light microscopy with Ångstrom precision deciphers structural conformations of PIEZO1
Cryogenic light microscopy with Ångstrom precision deciphers structural conformations of PIEZO1 Open
Despite the impressive progress in molecular biochemistry and biophysics, many questions regarding the conformational states of large (transmembrane) protein complexes persist. In the case of the PIEZO protein, investigations by cryogenic …
View article: Noncanonical WNT5A controls the activation of latent TGF-β to drive fibroblast activation and tissue fibrosis
Noncanonical WNT5A controls the activation of latent TGF-β to drive fibroblast activation and tissue fibrosis Open
Transforming growth factor β (TGF-β) signaling is a core pathway of fibrosis, but the molecular regulation of the activation of latent TGF-β remains incompletely understood. Here, we demonstrate a crucial role of WNT5A/JNK/ROCK signaling t…
View article: A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control
A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control Open
View article: A paintbrush for delivery of nanoparticles and small molecules to live cells with micrometer spatial and millisecond temporal control
A paintbrush for delivery of nanoparticles and small molecules to live cells with micrometer spatial and millisecond temporal control Open
Delivery of very small amounts of reagents to cells with micrometer spatial precision and millisecond time resolution is currently out of reach, but would strongly facilitate quantitative studies in cell biology. Here, we present a micropi…
View article: High-Precision Protein-Tracking With Interferometric Scattering Microscopy
High-Precision Protein-Tracking With Interferometric Scattering Microscopy Open
The mobility of proteins and lipids within the cell, sculpted oftentimes by the organization of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single p…
View article: Lipid Dynamics in Membranes Slowed Down by Transmembrane Proteins
Lipid Dynamics in Membranes Slowed Down by Transmembrane Proteins Open
Lipids and proteins, as essential components of biological cell membranes, exhibit a significant degree of freedom for different kinds of motions including lateral long-range mobility. Due to their interactions, they not only preserve the …
View article: High-precision protein-tracking with interferometric scattering microscopy
High-precision protein-tracking with interferometric scattering microscopy Open
The mobility of proteins and lipids within the cell, sculpted oftentimes by the organisation of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single p…
View article: Molecular crosstalk between Y5 receptor and neuropeptide Y drives liver cancer
Molecular crosstalk between Y5 receptor and neuropeptide Y drives liver cancer Open
Hepatocellular carcinoma (HCC) is clearly age-related and represents one of the deadliest cancer types worldwide. As a result of globally increasing risk factors including metabolic disorders, the incidence rates of HCC are still rising. H…
View article: Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1 Open
View article: Author Correction: Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo
Author Correction: Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo Open
View article: Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo
Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo Open
Connexins are the primary components of gap junctions, providing direct links between cells under many physiological processes. Here, we demonstrate that in addition to this canonical role, Connexins act as transcriptional regulators. We s…
View article: Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane
Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane Open
Much of the biological functions of a cell are dictated by the intricate motion of proteins within its membrane over a spatial range of nanometers to tens of micrometers and time intervals of microseconds to minutes. While this rich parame…
View article: Pgam5 released from damaged mitochondria induces mitochondrial biogenesis via Wnt signaling
Pgam5 released from damaged mitochondria induces mitochondrial biogenesis via Wnt signaling Open
Mitochondrial abundance is dynamically regulated and was previously shown to be increased by Wnt/β-catenin signaling. Pgam5 is a mitochondrial phosphatase which is cleaved by the rhomboid protease presenilin-associated rhomboid-like protei…
View article: Dishevelled Paralogs in Vertebrate Development: Redundant or Distinct?
Dishevelled Paralogs in Vertebrate Development: Redundant or Distinct? Open
Dishevelled (DVL) proteins are highly conserved in the animal kingdom and are important key players in β-Catenin-dependent and -independent Wnt signaling pathways. Vertebrate genomes typically comprise three DVL genes, DVL1, DVL2, and DVL3…
View article: EFhd2/Swiprosin-1 is a common genetic determinator for sensation-seeking/low anxiety and alcohol addiction
EFhd2/Swiprosin-1 is a common genetic determinator for sensation-seeking/low anxiety and alcohol addiction Open
View article: Connexin43 controls N-cadherin transcription during collective cell migration
Connexin43 controls N-cadherin transcription during collective cell migration Open
Connexins are the primary components of gap junctions, providing direct links between cells in many physiological processes, including cell migration and cancer metastasis. Exactly how cell migration is controlled by gap junctions remains …
View article: The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning
The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning Open
The scaffold protein Dishevelled is a central intracellular component of Wnt signaling pathways. Various kinases have been described that regulate and modulate Wnt signaling through phosphorylation of Dishevelled. However, besides the gene…
View article: Signaling pathways and tissue interactions in neural plate border formation
Signaling pathways and tissue interactions in neural plate border formation Open
The neural crest is a transient cell population that gives rise to various cell types of multiple tissues and organs in the vertebrate embryo. Neural crest cells arise from the neural plate border, a region localized at the lateral borders…
View article: β-catenin-independent WNT signaling and Ki67 in contrast to the estrogen receptor status are prognostic and associated with poor prognosis in breast cancer liver metastases
β-catenin-independent WNT signaling and Ki67 in contrast to the estrogen receptor status are prognostic and associated with poor prognosis in breast cancer liver metastases Open
Liver metastasis development in breast cancer patients is common and confers a poor prognosis. So far, the prognostic significance of surgical resection and clinical relevance of biomarker analysis in metastatic tissue have barely been inv…
View article: Differential requirement of bone morphogenetic protein receptors Ia (ALK3) and Ib (ALK6) in early embryonic patterning and neural crest development
Differential requirement of bone morphogenetic protein receptors Ia (ALK3) and Ib (ALK6) in early embryonic patterning and neural crest development Open
Background Bone morphogenetic proteins regulate multiple processes in embryonic development, including early dorso-ventral patterning and neural crest development. BMPs activate heteromeric receptor complexes consisting of type I and type …
View article: The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix
The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix Open
View article: Distinct functionality of dishevelled isoforms on Ca<sup>2+</sup>/calmodulin-dependent protein kinase 2 (CamKII) in<i>Xenopus</i>gastrulation
Distinct functionality of dishevelled isoforms on Ca<sup>2+</sup>/calmodulin-dependent protein kinase 2 (CamKII) in<i>Xenopus</i>gastrulation Open
CamKII is a novel binding partner of Arrb2/Dvl2 protein complexes and is required for convergent extension movements in Xenopus. CamKII physically and functionally interacts with Dvl2, whereas CamKII activity is antagonistically modulated …