Alexandre Hofer
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View article: Selective Photocatalytic C−H Oxidation of 5‐Methylcytosine in DNA
Selective Photocatalytic C−H Oxidation of 5‐Methylcytosine in DNA Open
Selective C−H activation on complex biological macromolecules is a key goal in the field of organic chemistry. It requires thermodynamically challenging chemical transformations to be delivered under mild, aqueous conditions. 5‐Methylcytos…
View article: Selective Photocatalytic C−H Oxidation of 5‐Methylcytosine in DNA
Selective Photocatalytic C−H Oxidation of 5‐Methylcytosine in DNA Open
Selective C−H activation on complex biological macromolecules is a key goal in the field of organic chemistry. It requires thermodynamically challenging chemical transformations to be delivered under mild, aqueous conditions. 5‐Methylcytos…
View article: The chemistry of branched condensed phosphates
The chemistry of branched condensed phosphates Open
View article: The Chemistry of Branched Condensed Phosphates
The Chemistry of Branched Condensed Phosphates Open
Our manuscript explores the uncharted territory of the linear branched ultraphosphates by developing and combining synthesis, purification, analysis, and computational approaches. This group of condensed phosphates as true constitutional i…
View article: Selective Chemical Functionalization at N6-Methyladenosine Residues in DNA Enabled by Visible-Light-Mediated Photoredox Catalysis
Selective Chemical Functionalization at N6-Methyladenosine Residues in DNA Enabled by Visible-Light-Mediated Photoredox Catalysis Open
Selective chemistry that modifies the structure of DNA and RNA is essential to understanding the role of epigenetic modifications. We report a visible-light-activated photocatalytic process that introduces a covalent modification at a C(sp…
View article: Selective Chemical Functionalization at N6 mdA Residues in DNA
Selective Chemical Functionalization at N6 mdA Residues in DNA Open
Selective chemistry that modifies the structure of DNA and RNA is essential to understanding the role of epigenetic modifications. We report a visible-light-activated photocatalytic process that introduces a covalent modification at a C(sp…
View article: The Significance of the Bifunctional Kinase/Phosphatase Activities of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for Coupling Inositol Pyrophosphate Cell Signaling to Cellular Phosphate Homeostasis
The Significance of the Bifunctional Kinase/Phosphatase Activities of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for Coupling Inositol Pyrophosphate Cell Signaling to Cellular Phosphate Homeostasis Open
Proteins responsible for Pi homeostasis are critical for all life. In Saccharomyces cerevisiae, extracellular [Pi] is “sensed” by the inositol-hexakisphosphate kinase (IP6K) that synthesizes the intracellular inositol pyrophosphate 5-dipho…
View article: <i>Arabidopsis</i> ITPK1 and ITPK2 Have an Evolutionarily Conserved Phytic Acid Kinase Activity
<i>Arabidopsis</i> ITPK1 and ITPK2 Have an Evolutionarily Conserved Phytic Acid Kinase Activity Open
Diphospho-myo-inositol polyphosphates, also termed inositol pyrophosphates, are molecular messengers containing at least one high-energy phosphoanhydride bond and regulate a wide range of cellular processes in eukaryotes. While inos…
View article: Screening a Protein Array with Synthetic Biotinylated Inorganic Polyphosphate To Define the Human PolyP-ome
Screening a Protein Array with Synthetic Biotinylated Inorganic Polyphosphate To Define the Human PolyP-ome Open
Phenotypes are established by tight regulation on protein functions. This regulation can be mediated allosterically, through protein binding, and covalently, through post-translational modification (PTM). The integration of an ever-increas…
View article: Substrate recognition and mechanism revealed by ligand-bound polyphosphate kinase 2 structures
Substrate recognition and mechanism revealed by ligand-bound polyphosphate kinase 2 structures Open
Significance Polyphosphate kinases (PPKs) are involved in many metabolic processes in bacteria, including pathogenic species. As these enzymes are not present in animals, they are a prime target for the development of novel antibiotics. Th…
View article: A 1-phytase type III effector interferes with plant hormone signaling
A 1-phytase type III effector interferes with plant hormone signaling Open
View article: Inositol Pyrophosphate Specificity of the SPX-Dependent Polyphosphate Polymerase VTC
Inositol Pyrophosphate Specificity of the SPX-Dependent Polyphosphate Polymerase VTC Open
The free energy of nucleotide hydrolysis depends on phosphate concentration. Cells regulate cytosolic phosphate levels by orchestrating phosphate acquisition and storage through inositol pyrophosphates (PP-InsP) and SPX domains. Here, we r…
View article: The Significance of the Bifunctional Kinase/Phosphatase Activities of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for Coupling Inositol Pyrophosphate Cell Signaling to Cellular Phosphate Homeostasis
The Significance of the Bifunctional Kinase/Phosphatase Activities of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for Coupling Inositol Pyrophosphate Cell Signaling to Cellular Phosphate Homeostasis Open
Proteins responsible for Pi homeostasis are critical for all life. In Saccharomyces cerevisiae, extracellular [Pi] is "sensed" by the inositol-hexakisphosphate kinase (IP6K) that synthesizes the intracellular i…
View article: Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry
Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry Open
Mass spectrometry-based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically-altered enzyme-cofactor systems. We describe …