2025-03-11
“The <i>B. subtilis</i> replicative polymerases bind the sliding clamp with different strengths to tune replication processivity and fidelity”
2025-03-11 • Luke G. O’Neal, Madeline N. Drucker, N. Chin Lai, Alfonso Clemente, Arezoo Campbell, Lindsey E. Way, Sinwoo Hong, Emily E. Holmes, Sarah J. Rancic,...
Abstract Ring-shaped sliding clamp proteins are essential components of the replication machinery, the replisome, across all domains of life. In bacteria, DNA polymerases bind the sliding clamp, DnaN, through conserved short peptide sequences called clamp-binding motifs. Clamp binding increases the processivity and rate of DNA synthesis and is generally required for polymerase activity. The current understanding of clamp-polymerase interactions was elucidated in the model bacterium Escherichia coli , which has a s…