Alfonso Espada
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View article: Droplet microfluidic hydrogen/deuterium exchange for investigating protein dynamics with millisecond precision
Droplet microfluidic hydrogen/deuterium exchange for investigating protein dynamics with millisecond precision Open
Hydrogen/deuterium exchange (HDX) methods for studying protein dynamics would benefit from millisecond-scale incubations to probe intrinsically disordered proteins, highly dynamic regions and conformation changes. Here we investigate dropl…
View article: Population-Scale Studies of Protein S Abnormalities and Thrombosis
Population-Scale Studies of Protein S Abnormalities and Thrombosis Open
Importance Clinical decision-making in thrombotic disorders is impeded by long-standing uncertainty regarding the magnitude of venous and arterial thrombosis risk associated with low protein S. Population-scale multiomic datasets offer an …
View article: Rare Germline Loss-of-Function Variants in C1-Inhibitor (<i>SERPING1</i>) Are Associated with Venous Thromboembolism in Population-Scale Sequencing Datasets
Rare Germline Loss-of-Function Variants in C1-Inhibitor (<i>SERPING1</i>) Are Associated with Venous Thromboembolism in Population-Scale Sequencing Datasets Open
Background: Hereditary angioedema (HAE) is a rare condition caused by autosomal dominant mutations in SERPING1 that result in deficiency or dysfunction of its gene product, C1 inhibitor (C1INH). C1INH is the main physiologic inhibitor of s…
View article: Crystal structure of active CDK4-cyclin D and mechanistic basis for abemaciclib efficacym
Crystal structure of active CDK4-cyclin D and mechanistic basis for abemaciclib efficacym Open
Despite the therapeutic relevance of CDK4/6, the crystal structure of active CDK4 has yet to be elucidated, and the mechanistic rationale for sustained cell cycle inhibition is not fully understood. Here, we describe the co-crystal structu…
View article: Crystal structure of active CDK4-cyclin D and mechanistic basis for abemaciclib efficacy
Crystal structure of active CDK4-cyclin D and mechanistic basis for abemaciclib efficacy Open
Despite the biological and therapeutic relevance of CDK4/6 for the treatment of HR+, HER2- advanced breast cancer, the detailed mode of action of CDK4/6 inhibitors is not completely understood. Of particular interest, phosphorylation of CD…
View article: HDX Workbench data export for "The Angiopoietin-Like Protein 3 and 8 Complex Interacts with Lipoprotein Lipase and Induces LPL Cleavage"
HDX Workbench data export for "The Angiopoietin-Like Protein 3 and 8 Complex Interacts with Lipoprotein Lipase and Induces LPL Cleavage" Open
Full CSV data export of the HDX experiment results analyzed by the software HDX Workbench for the manuscript The Angiopoietin-Like Protein 3 and 8 Complex Interacts with Lipoprotein Lipase and Induces LPL Cleavage
View article: Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage
Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage Open
Hundreds of target specific peptides are routinely discovered by peptide display platforms. However, due to the high cost of peptide synthesis only a limited number of peptides are chemically made for further analysis. Here we describe an …
View article: HDX Workbench data export for The Complex of Angiopoietin-Like Protein 3 and 8 Interacts with Lipoprotein Lipase and Induces LPL Inhibition.
HDX Workbench data export for The Complex of Angiopoietin-Like Protein 3 and 8 Interacts with Lipoprotein Lipase and Induces LPL Inhibition. Open
Full CSV data export of the HDX experiment results analyzed by the software HDX Workbench for the manuscript The Complex of Angiopoietin-Like Protein 3 and 8 Interacts with Lipoprotein Lipase and Induces LPL Inhibition. The dataset include…
View article: Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Centroid Data Measured between 3.6 °C and 25.4 °C for the Fab Fragment of NISTmAb
Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Centroid Data Measured between 3.6 °C and 25.4 °C for the Fab Fragment of NISTmAb Open
The spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the FabFragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS…
View article: Interlaboratory Comparison of Hydrogen–Deuterium Exchange Mass Spectrometry Measurements of the Fab Fragment of NISTmAb
Interlaboratory Comparison of Hydrogen–Deuterium Exchange Mass Spectrometry Measurements of the Fab Fragment of NISTmAb Open
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is an established, powerful tool for investigating protein-ligand interactions, protein folding, and protein dynamics. However, HDX-MS is still an emergent tool for quality control of …
View article: Deciphering Binding Interactions of IL-23R with HDX-MS: Mapping Protein and Macrocyclic Dodecapeptide Ligands
Deciphering Binding Interactions of IL-23R with HDX-MS: Mapping Protein and Macrocyclic Dodecapeptide Ligands Open
Molecular characterization of the binding epitope of IL-23R and its cognate cytokine IL-23 is paramount to understand the role in autoimmune diseases and to support the discovery of new inhibitors of this protein-protein interaction. Our r…
View article: Utilization of peptide phage display to investigate hotspots on IL-17A and what it means for drug discovery
Utilization of peptide phage display to investigate hotspots on IL-17A and what it means for drug discovery Open
To date, IL-17A antibodies remain the only therapeutic approach to correct the abnormal activation of the IL-17A/IL-17R signaling complex. Why is it that despite the remarkable success of IL-17 antibodies, there is no small molecule antago…