Alicia V. Lis
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View article: Engineered Reversal of Function in Glycolytic Yeast Promoters
Engineered Reversal of Function in Glycolytic Yeast Promoters Open
Promoters are key components of cell factory design, allowing precise expression of genes in a heterologous pathway. Several commonly used promoters in yeast cell factories belong to glycolytic genes, highly expressed in actively growing y…
View article: Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae
Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae Open
View article: Engineered reversal of function in glycolytic yeast promoters
Engineered reversal of function in glycolytic yeast promoters Open
Promoters are key components of cell factory design, allowing precise expression of genes in a heterologous pathway. Several commonly-used promoters in yeast cell factories belong to glycolytic genes, highly expressed in actively-growing y…
View article: CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9
CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9 Open
Here we describe a method for robust directed evolution using mutagenesis of large sequence spaces in their genomic contexts. The method employs error-prone PCR and Cas9-mediated genome integration of mutant libraries of large-sized donor …
View article: Specific <i>Arabidopsis thaliana</i> malic enzyme isoforms can provide anaplerotic pyruvate carboxylation function in <i>Saccharomyces cerevisiae</i>
Specific <i>Arabidopsis thaliana</i> malic enzyme isoforms can provide anaplerotic pyruvate carboxylation function in <i>Saccharomyces cerevisiae</i> Open
NAD(P)‐malic enzyme (NAD(P)‐ME) catalyzes the reversible oxidative decarboxylation of malate to pyruvate, CO 2 , and NAD(P)H and is present as a multigene family in Arabidopsis thaliana . The carboxylation reaction catalyzed by purified re…
View article: Rational and Evolutionary Engineering of Industrial Saccharomyces Cerevisiae Strains for Production of Chemicals from Xylose-Rich Feedstocks
Rational and Evolutionary Engineering of Industrial Saccharomyces Cerevisiae Strains for Production of Chemicals from Xylose-Rich Feedstocks Open