Alin Rai
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View article: Multi-omics identify hallmark protein and lipid features of small extracellular vesicles circulating in human plasma
Multi-omics identify hallmark protein and lipid features of small extracellular vesicles circulating in human plasma Open
Extracellular vesicles (EVs) are an essential signalling entity in human plasma implicated in health and disease. Still, their core protein and lipid componentry, which lie at the centre of EV form and function, remain poorly defined. Here…
View article: Extracellular vesicle profiling reveals novel autism signatures in patient-derived forebrain organoids
Extracellular vesicle profiling reveals novel autism signatures in patient-derived forebrain organoids Open
Autism Spectrum Disorder (ASD) affects 1 percent of the world's population with an increased prevalence of 178 percent since 2000. Although altered synaptic function putatively accounts for many of the abnormalities seen in ASD, the specif…
View article: Oncogenic H‐Ras Reprograms Madin‐Darby Canine Kidney (MDCK) Cell‐Derived Midbody Remnant Proteome Following Epithelial‐Mesenchymal Transition
Oncogenic H‐Ras Reprograms Madin‐Darby Canine Kidney (MDCK) Cell‐Derived Midbody Remnant Proteome Following Epithelial‐Mesenchymal Transition Open
Epithelial‐mesenchymal transition (EMT) is a fundamental, dynamic cellular process involved in embryonic development, metastasis, organ fibrosis, and tissue regeneration. To define the molecular landscape of secreted midbody remnants (MBRs…
View article: Dynamic Proteome Landscape During Preimplantation Human Embryo Development and Trophectoderm Stem Cell‐Differentiation
Dynamic Proteome Landscape During Preimplantation Human Embryo Development and Trophectoderm Stem Cell‐Differentiation Open
Embryo development involves fertilization of a mature ovum, which, after sequential cell divisions (2‐,4‐8‐cells and morula), undergoes differentiation into implantation competent blastocyst. The blastocyst comprises of inner cell mass sur…
View article: Oncogenic H-Ras Reprograms Madin-Darby Canine Kidney (MDCK) Cell-derived Midbody Remnant Proteins Following Epithelial-Mesenchymal Transition
Oncogenic H-Ras Reprograms Madin-Darby Canine Kidney (MDCK) Cell-derived Midbody Remnant Proteins Following Epithelial-Mesenchymal Transition Open
Epithelial-mesenchymal transition (EMT) is a highly conserved morphogenic process that allows highly polarized, immotile epithelial cells to transform into motile mesenchymal cells: it is a fundamental cellular process involved in embryoni…
View article: Proteomics and Machine Learning–Based Approach to Decipher Subcellular Proteome of Mouse Heart
Proteomics and Machine Learning–Based Approach to Decipher Subcellular Proteome of Mouse Heart Open
Protein compartmentalization to distinctive subcellular niches is critical for cardiac function and homeostasis. Here, we employed a rapid and robust workflow based on differential centrifugal-based fractionation with mass spectrometry-bas…
View article: The Proteomic Landscape of the Coronary Accessible Heart Cell Surfaceome
The Proteomic Landscape of the Coronary Accessible Heart Cell Surfaceome Open
Cell surface proteins (surfaceome) represent key signalling and interaction molecules for therapeutic targeting, biomarker profiling and cellular phenotyping in physiological and pathological states. Here, we employed coronary artery perfu…
View article: Differential effects of physiological agonists on the proteome of platelet‐derived extracellular vesicles
Differential effects of physiological agonists on the proteome of platelet‐derived extracellular vesicles Open
Arterial thrombosis manifesting as heart attack and stroke is the leading cause of death worldwide. Platelets are central mediators of thrombosis that can be activated through multiple activation pathways. Platelet‐derived extracellular ve…
View article: Multi-omics discovery of hallmark protein and lipid features of circulating small extracellular vesicles in humans
Multi-omics discovery of hallmark protein and lipid features of circulating small extracellular vesicles in humans Open
Extracellular vesicles (EVs) are now being increasingly recognized as an essential signaling entity in human plasma, linking them to health and various diseases. Still, their core protein and lipid componentry, which lie at the center of E…
View article: Transcriptomic analysis and fusion gene identifications of midbody remnants released from colorectal cancer cells reveals they are molecularly distinct from exosomes and microparticles
Transcriptomic analysis and fusion gene identifications of midbody remnants released from colorectal cancer cells reveals they are molecularly distinct from exosomes and microparticles Open
Previously, we reported that human primary (SW480) and metastatic (SW620) colorectal (CRC) cells release three classes of membrane‐encapsulated extracellular vesicles (EVs); midbody remnants (MBRs), exosomes (Exos), and microparticles (MPs…
View article: HB‐EGF‐loaded nanovesicles enhance trophectodermal spheroid attachment and invasion
HB‐EGF‐loaded nanovesicles enhance trophectodermal spheroid attachment and invasion Open
The ability of trophectodermal cells (outer layer of the embryo) to attach to the endometrial cells and subsequently invade the underlying matrix are critical stages of embryo implantation during successful pregnancy establishment. Extrace…
View article: Cardiomyocyte intercellular signalling increases oxidative stress and reprograms the global‐ and phospho‐proteome of cardiac fibroblasts
Cardiomyocyte intercellular signalling increases oxidative stress and reprograms the global‐ and phospho‐proteome of cardiac fibroblasts Open
Pathological reprogramming of cardiomyocyte and fibroblast proteome landscapes drive the initiation and progression of cardiac fibrosis. Although the secretome of dysfunctional cardiomyocytes is emerging as an important driver of pathologi…
View article: Rapid and in‐depth proteomic profiling of small extracellular vesicles for ultralow samples
Rapid and in‐depth proteomic profiling of small extracellular vesicles for ultralow samples Open
The integration of robust single‐pot, solid‐phase‐enhanced sample preparation with powerful liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) is routinely used to define the extracellular vesicle (EV) proteome landscape and underly…
View article: Rapid generation of functional nanovesicles from human trophectodermal cells for embryo attachment and outgrowth
Rapid generation of functional nanovesicles from human trophectodermal cells for embryo attachment and outgrowth Open
Extracellular vesicles (EVs) are important mediators of embryo attachment and outgrowth critical for successful implantation. While EVs have garnered immense interest in their therapeutic potential in assisted reproductive technology by im…
View article: Comparative proteomic analysis of three major extracellular vesicle classes secreted from human primary and metastatic colorectal cancer cells: Exosomes, microparticles, and shed midbody remnants
Comparative proteomic analysis of three major extracellular vesicle classes secreted from human primary and metastatic colorectal cancer cells: Exosomes, microparticles, and shed midbody remnants Open
Cell‐derived extracellular vesicles (EVs) are evolutionary‐conserved secretory organelles that, based on their molecular composition, are important intercellular signaling regulators. At least three classes of circulating EVs are known bas…
View article: Quantitative proteomic landscape of unstable atherosclerosis identifies molecular signatures and therapeutic targets for plaque stabilization
Quantitative proteomic landscape of unstable atherosclerosis identifies molecular signatures and therapeutic targets for plaque stabilization Open
View article: A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood
A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood Open
Pluripotent stem cells are key players in regenerative medicine. Embryonic pluripotent stem cells, despite their significant advantages, are associated with limitations such as their inadequate availability and the ethical dilemmas in thei…
View article: Omics insights into extracellular vesicles in embryo implantation and their therapeutic utility
Omics insights into extracellular vesicles in embryo implantation and their therapeutic utility Open
Implantation success relies on intricate interplay between the developing embryo and the maternal endometrium. Extracellular vesicles (EVs) represent an important player of this intercellular signalling through delivery of functional cargo…
View article: Endometrial small extracellular vesicles regulate human trophectodermal cell invasion by reprogramming the phosphoproteome landscape
Endometrial small extracellular vesicles regulate human trophectodermal cell invasion by reprogramming the phosphoproteome landscape Open
A series of cyclical events within the uterus are crucial for pregnancy establishment. These include endometrial regeneration following menses, under the influence of estrogen (proliferative phase), then endometrial differentiation driven …
View article: Scalable Generation of Nanovesicles from Human-Induced Pluripotent Stem Cells for Cardiac Repair
Scalable Generation of Nanovesicles from Human-Induced Pluripotent Stem Cells for Cardiac Repair Open
Extracellular vesicles (EVs) from stem cells have shown significant therapeutic potential to repair injured cardiac tissues and regulate pathological fibrosis. However, scalable generation of stem cells and derived EVs for clinical utility…
View article: Proteomic dissection of large extracellular vesicle surfaceome unravels interactive surface platform
Proteomic dissection of large extracellular vesicle surfaceome unravels interactive surface platform Open
The extracellular vesicle (EV) surface proteome (surfaceome) acts as a fundamental signalling gateway by bridging intra‐ and extracellular signalling networks, dictates EVs’ capacity to communicate and interact with their environment, and …
View article: Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Open
Doublecortin‐like kinase 1 (DCLK1) is a putative cancer stem cell marker, a promising diagnostic and prognostic maker for malignant tumors and a proposed driver gene for gastric cancer (GC). DCLK1 overexpression in a majority of solid canc…
View article: Proteome characterisation of extracellular vesicles isolated from heart
Proteome characterisation of extracellular vesicles isolated from heart Open
Cardiac intercellular communication is critical for heart function and often dysregulated in cardiovascular diseases. While cardiac extracellular vesicles (cEVs) are emerging mediators of signalling, their isolation remains a technical cha…
View article: Proteome reprogramming of endometrial epithelial cells by human trophectodermal small extracellular vesicles reveals key insights into embryo implantation
Proteome reprogramming of endometrial epithelial cells by human trophectodermal small extracellular vesicles reveals key insights into embryo implantation Open
Embryo implantation into the receptive endometrium is critical in pregnancy establishment, initially requiring reciprocal signalling between outer layer of the blastocyst (trophectoderm cells) and endometrial epithelium; however, factors r…
View article: Secreted midbody remnants are a class of extracellular vesicles molecularly distinct from exosomes and microparticles
Secreted midbody remnants are a class of extracellular vesicles molecularly distinct from exosomes and microparticles Open
During the final stages of cell division, newly-formed daughter cells remain connected by a thin intercellular bridge containing the midbody (MB), a microtubule-rich organelle responsible for cytokinetic abscission. Following cell division…
View article: Transglutaminase‐2, RNA‐binding proteins and mitochondrial proteins selectively traffic to MDCK cell‐derived microvesicles following H‐Ras‐induced epithelial–mesenchymal transition
Transglutaminase‐2, RNA‐binding proteins and mitochondrial proteins selectively traffic to MDCK cell‐derived microvesicles following H‐Ras‐induced epithelial–mesenchymal transition Open
Epithelial–mesenchymal transition (EMT) describes an evolutionary conserved morphogenic process defined by loss of epithelial characteristics and acquisition of mesenchymal phenotype, and altered patterns of intercellular communication, le…
View article: Proteomic profiling of human uterine extracellular vesicles reveal dynamic regulation of key players of embryo implantation and fertility during menstrual cycle
Proteomic profiling of human uterine extracellular vesicles reveal dynamic regulation of key players of embryo implantation and fertility during menstrual cycle Open
Endometrial extracellular vesicles (EVs) are emerging as important players in reproductive biology. However, how their proteome is regulated throughout the menstrual cycle is not known. Such information can provide novel insights into biol…
View article: A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes
A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes Open
View article: Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast‐Led Cancer Invasion
Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast‐Led Cancer Invasion Open
In localized tumors, basement membrane (BM) prevents invasive outgrowth of tumor cells into surrounding tissues. When carcinomas become invasive, cancer cells either degrade BM or reprogram stromal fibroblasts to breach BM barrier and lead…
View article: Human Endometrial Extracellular Vesicles Functionally Prepare Human Trophectoderm Model for Implantation: Understanding Bidirectional Maternal‐Embryo Communication
Human Endometrial Extracellular Vesicles Functionally Prepare Human Trophectoderm Model for Implantation: Understanding Bidirectional Maternal‐Embryo Communication Open
Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial–embryo cross talk …