Aline Herger
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View article: Virulence on Pm4 kinase-based resistance is determined by two divergent wheat powdery mildew effectors
Virulence on Pm4 kinase-based resistance is determined by two divergent wheat powdery mildew effectors Open
The wheat resistance gene Pm4 encodes a kinase fusion protein and has gained particular attention as it confers race-specific resistance against two major wheat pathogens: powdery mildew and blast. Here we describe the identification of Av…
A reference metagenome sequence of the lichen Cladonia rangiformis Open
Background Lichens are an ancient symbiosis comprising the thalli of lichen-forming fungi, their photoautotrophic partners, and their microbiome. So far, they were poorly studied at the genome sequence level. Here, we present a reference m…
View article: An HMA-like integrated domain in the wheat tandem kinase WTK4 recognises an RNase-like pathogen effector
An HMA-like integrated domain in the wheat tandem kinase WTK4 recognises an RNase-like pathogen effector Open
Proteins with a tandem kinase structure have recently emerged as new players in race-specific resistance in cereal crops. However, the molecular understanding of these novel immune receptors’ resistance mechanisms is limited by the lack of…
View article: Virulence on Pm4 kinase-based resistance is determined by two divergent wheat powdery mildew effectors
Virulence on Pm4 kinase-based resistance is determined by two divergent wheat powdery mildew effectors Open
The wheat resistance gene Pm4 encodes a kinase fusion protein and has gained particular attention as it confers race-specific resistance against two major wheat pathogens: powdery mildew and blast. Here, we describe the identification of A…
View article: The CATION CALCIUM EXCHANGER 4 (CCX4) regulates LRX1-related root hair development through Ca <sup>2+</sup> homeostasis
The CATION CALCIUM EXCHANGER 4 (CCX4) regulates LRX1-related root hair development through Ca <sup>2+</sup> homeostasis Open
Summary Calcium, as a cellular second messenger, is essential for plant growth. A tip-focused Ca 2+ gradient in polarized cells is considered to drive cell expansion. The cell wall polysaccharide pectin is a major Ca 2+ binding structure a…
View article: A reference metagenome sequence of the lichen <i>Cladonia rangiformis</i>
A reference metagenome sequence of the lichen <i>Cladonia rangiformis</i> Open
Summary ● Lichens are an ancient symbiosis comprising the thalli of lichen-forming fungi, their photoautotrophic partners and their microbiome. So far, they were poorly studied at the genome sequence level. Here, we present a reference met…
View article: Histidine limitation alters plant development and influences the TOR network
Histidine limitation alters plant development and influences the TOR network Open
Plant growth depends on growth regulators, nutrient availability, and amino acid levels, all of which influence cell wall formation and cell expansion. Cell wall integrity and structures are surveyed and modified by a complex array of cell…
View article: Histidine limitation causes alteration in the TOR network and plant development
Histidine limitation causes alteration in the TOR network and plant development Open
Plant growth depends on growth regulators, nutrient availability, and amino acids levels. The TOR (Target of Rapamycin) network senses these parameters and influences cell wall formation and expansion accordingly. Cell wall integrity and s…
View article: Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module
Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module Open
Plant cell growth involves coordination of numerous processes and signaling cascades among the different cellular compartments to concomitantly enlarge the protoplast and the surrounding cell wall. The cell wall integrity-sensing process i…
View article: Growth-inhibiting effects of the unconventional plant APYRASE 7 of <i>Arabidopsis thaliana</i> influences the LRX1/FER/RALF growth regulatory module
Growth-inhibiting effects of the unconventional plant APYRASE 7 of <i>Arabidopsis thaliana</i> influences the LRX1/FER/RALF growth regulatory module Open
Plant cell growth involves coordination of numerous processes and signaling cascades among the different cellular compartments to concomitantly enlarge the protoplast and the surrounding cell wall. In Arabidopsis thaliana , the cell wall i…
View article: Defects in Cell Wall Differentiation of the Arabidopsis Mutant rol1-2 Is Dependent on Cyclin-Dependent Kinase CDK8
Defects in Cell Wall Differentiation of the Arabidopsis Mutant rol1-2 Is Dependent on Cyclin-Dependent Kinase CDK8 Open
Plant cells are encapsulated by cell walls whose properties largely determine cell growth. We have previously identified the rol1-2 mutant, which shows defects in seedling root and shoot development. rol1-2 is affected in the Rhamnose synt…
View article: Fluorescence of <i>AUN1<sup>D94N</sup>-YFP</i> and <i>MRI<sup>R240C</sup>-YFP</i> transgenic lines.
Fluorescence of <i>AUN1<sup>D94N</sup>-YFP</i> and <i>MRI<sup>R240C</sup>-YFP</i> transgenic lines. Open
Transgenic lines expressing MRIR240C-YFP or AUN1D94N-YFP show YFP fluorescence at the plasma membrane/cytoplasm and in the nucleus/cytoplasm, respectively. Individual root hairs (A) and entire roots (B) are shown. (B) When expressing MRIR2…
View article: RT-PCR confirming expression of the transgenes.
RT-PCR confirming expression of the transgenes. Open
Total RNA was extracted from 7-days old seedlings and transgene-specific RT-PCR was performed to confirm expression of the transgenes. In all experiments, Actin2 was used as endogenous control for comparable amounts of RNA in all samples a…
View article: LRX1<sup>ΔNT</sup> protein levels in transgenic <i>lrx1</i> and <i>lrx1 lrx2</i> mutants.
LRX1<sup>ΔNT</sup> protein levels in transgenic <i>lrx1</i> and <i>lrx1 lrx2</i> mutants. Open
Root material of 10 days-old seedlings was used to detect LRX1ΔNT with the anti-cmyc antibody 9E10. Protein levels in transgenic lrx1 vary but do not correlate with the inconsistent phenotypes observed in these lines as shown in Fig 4C. Tr…
View article: Interaction of LRXs and FER in yeast-two-hybrid assays.
Interaction of LRXs and FER in yeast-two-hybrid assays. Open
The yeast strain PJ69-4A was transformed with pGADT7-FERECD and pGBKT7-LRRs (encoding the LRR domain of the different LRXs as indicated), and with either of the constructs with empty plasmids for the negative controls. The clear difference…
View article: Overlapping functions and protein-protein interactions of LRR-extensins in Arabidopsis
Overlapping functions and protein-protein interactions of LRR-extensins in Arabidopsis Open
Plant cell growth requires the coordinated expansion of the protoplast and the cell wall, which is controlled by an elaborate system of cell wall integrity (CWI) sensors linking the different cellular compartments. LRR-eXtensins (LRXs) are…
View article: Complementation of <i>lrx345</i> and <i>lrx1</i> mutants.
Complementation of <i>lrx345</i> and <i>lrx1</i> mutants. Open
(A) lrx345 triple mutant plants are smaller at flowering stage than the wild type (Col). This phenotype is complemented by the 35S:LRX1 construct. (B) The lrx1 root hair defect is complemented by the LRX chimeric constructs. Bar: 1cm (A); …
View article: LRR-extensins of vegetative tissues are a functionally conserved family of RALF1 receptors interacting with the receptor kinase FERONIA
LRR-extensins of vegetative tissues are a functionally conserved family of RALF1 receptors interacting with the receptor kinase FERONIA Open
Plant cell growth requires the coordinated expansion of the protoplast and the cell wall that confers mechanical stability to the cell. An elaborate system of cell wall integrity sensors monitors cell wall structures and conveys informatio…
View article: Mutations in the Arabidopsis ROL17/isopropylmalate synthase 1 locus alter amino acid content, modify the TOR network, and suppress the root hair cell development mutant lrx1
Mutations in the Arabidopsis ROL17/isopropylmalate synthase 1 locus alter amino acid content, modify the TOR network, and suppress the root hair cell development mutant lrx1 Open
The growth and development of organisms must be tightly controlled and adjusted to nutrient availability and metabolic activities. The Target of Rapamycin (TOR) network is a major control mechanism in eukaryotes and influences processes su…
View article: LRX Proteins Play a Crucial Role in Pollen Grain and Pollen Tube Cell Wall Development
LRX Proteins Play a Crucial Role in Pollen Grain and Pollen Tube Cell Wall Development Open
Leu-rich repeat extensins (LRXs) are chimeric proteins containing an N-terminal Leu-rich repeat (LRR) and a C-terminal extensin domain. LRXs are involved in cell wall formation in vegetative tissues and required for plant growth. However, …
View article: LRX Proteins play a crucial role in pollen grain and pollen tube cell wall development
LRX Proteins play a crucial role in pollen grain and pollen tube cell wall development Open
Leucine-rich repeat extensins (LRXs) are chimeric proteins containing an N-terminal leucine-rich repeat (LRR) and a C-terminal extensin domain. LRXs are involved in cell wall formation in vegetative tissues and required for plant growth. H…