Alison Tedcastle
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View article: Tiled PCR Amplification for Poliovirus Whole-Genome Sequencing v1
Tiled PCR Amplification for Poliovirus Whole-Genome Sequencing v1 Open
This protocol describes the amplification and whole-genome sequencing of poliovirus from clinical or environmental RNA extracts using an overlapping tiled amplicon strategy. Four overlapping PCR products are generated from viral RNA via RT…
View article: Direct Detection of poliovirus and Nanopore Sequencing (DDNS) - Environmental Samples v1
Direct Detection of poliovirus and Nanopore Sequencing (DDNS) - Environmental Samples v1 Open
This protocol covers amplification of poliovirus nucleic acid from concentrated sewage samples and the subsequent sequencing of barcoded VP1 amplicons. The protocol was successfully tested on concentrates prepared with two-phase separation…
View article: Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v2
Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v2 Open
This method describes large volume nucleic acid purification from sewage concentrates starting with 1.2 mL of sample using MagMAX ‱ Viral RNA Isolation Kit. Start by preparing the reagents required for the number of samples extracted, incl…
View article: Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v1
Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v1 Open
This method describes large volume nucleic acid purification from sewage concentrates starting with 1.2 mL of sample using MagMAX ‱ Viral RNA Isolation Kit. To perform automated extraction, follow steps in Workflow A, and for manual extrac…
View article: Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v2
Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v2 Open
This method describes large volume nucleic acid purification from sewage concentrates starting with 1.2 mL of sample using MagMAX ‱ Viral RNA Isolation Kit. Start by preparing the reagents required for the number of samples extracted, incl…
View article: Production of antigenically stable enterovirus A71 virus-like particles in Pichia pastoris as a vaccine candidate
Production of antigenically stable enterovirus A71 virus-like particles in Pichia pastoris as a vaccine candidate Open
Enterovirus A71 (EVA71) causes widespread disease in young children with occasional fatal consequences. In common with other picornaviruses, both empty capsids (ECs) and infectious virions are produced during the viral lifecycle. While ini…
View article: Production of antigenically stable enterovirus A71 virus-like particles in<i>Pichia pastoris</i>as a vaccine candidate
Production of antigenically stable enterovirus A71 virus-like particles in<i>Pichia pastoris</i>as a vaccine candidate Open
Enterovirus A71 (EVA71) causes widespread disease in young children with occasional fatal consequences. In common with other picornaviruses, both empty capsids (ECs) and infectious virions are produced during the viral lifecycle. While ini…
View article: Sustained detection of type 2 poliovirus in London sewage between February and July, 2022, by enhanced environmental surveillance
Sustained detection of type 2 poliovirus in London sewage between February and July, 2022, by enhanced environmental surveillance Open
View article: Detection of Enterovirus D68 in Wastewater Samples from the UK between July and November 2021
Detection of Enterovirus D68 in Wastewater Samples from the UK between July and November 2021 Open
Infection with enterovirus D68 (EV-D68) has been linked with severe neurological disease such as acute flaccid myelitis (AFM) in recent years. However, active surveillance for EV-D68 is lacking, which makes full assessment of this associat…
View article: Cross-Antigenicity between EV71 Sub-Genotypes: Implications for Vaccine Efficacy
Cross-Antigenicity between EV71 Sub-Genotypes: Implications for Vaccine Efficacy Open
Enterovirus A-71 (EV71) is a global, highly contagkkious pathogen responsible for severe cases of hand-food-mouth-disease (HFMD). The use of vaccines eliciting cross neutralizing antibodies (NTAbs) against the different circulating EV71 su…
View article: Differences in Antigenic Structure of Inactivated Polio Vaccines Made From Sabin Live-Attenuated and Wild-Type Poliovirus Strains: Impact on Vaccine Potency Assays
Differences in Antigenic Structure of Inactivated Polio Vaccines Made From Sabin Live-Attenuated and Wild-Type Poliovirus Strains: Impact on Vaccine Potency Assays Open
Homologous references are required to measure the antigen content of IPV products consistently. The first World Health Organization international standard for sIPV was established, with new, specific Sabin D-antigen units assigned.
View article: Oncolytic Group B Adenovirus Enadenotucirev Mediates Non-apoptotic Cell Death with Membrane Disruption and Release of Inflammatory Mediators
Oncolytic Group B Adenovirus Enadenotucirev Mediates Non-apoptotic Cell Death with Membrane Disruption and Release of Inflammatory Mediators Open
View article: Actin-resistant DNAse I Expression From Oncolytic Adenovirus Enadenotucirev Enhances Its Intratumoral Spread and Reduces Tumor Growth
Actin-resistant DNAse I Expression From Oncolytic Adenovirus Enadenotucirev Enhances Its Intratumoral Spread and Reduces Tumor Growth Open