Allison P. Manalo
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View article: Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light
Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light Open
Background autofluorescence is enhanced in human tissue relative to small animals and presents a barrier to fully realizing the potential of novel multiplex methods in human studies. In particular, lipofuscin (LF) is an interfering pigment…
View article: Kappa opioids inhibit spinal output neurons to suppress itch
Kappa opioids inhibit spinal output neurons to suppress itch Open
Itch is a protective sensation that drives scratching. Although specific cell types have been proposed to underlie itch, the neural basis for itch remains unclear. Here, we used two-photon Ca 2+ imaging of the dorsal horn to visualize neur…
View article: Multiplex fluorescence in situ hybridization using RNAScope on paraffin embedded human nervous system tissue with optional photobleaching for autofluorescence mitigation v1
Multiplex fluorescence in situ hybridization using RNAScope on paraffin embedded human nervous system tissue with optional photobleaching for autofluorescence mitigation v1 Open
This protocol is to perform the RNAScope in situ hybridization procedure in human central nervous system tissue with modifications from our newly established method to reduce endogenous autofluorescence from lipofuscin. This procedure shou…
View article: Identification of a convergent spinal neuron population that encodes itch
Identification of a convergent spinal neuron population that encodes itch Open
Summary Itch is a protective sensation that drives scratching. Although specific cell types have been proposed to underlie itch, the neural circuit basis for itch remains unclear. Here, we used two-photon Ca 2+ imaging of the dorsal horn t…
View article: Expression pattern analysis and characterization of the hereditary sensory and autonomic neuropathy 2 A (HSAN2A) gene with no lysine kinase (WNK1) in human dorsal root ganglion
Expression pattern analysis and characterization of the hereditary sensory and autonomic neuropathy 2 A (HSAN2A) gene with no lysine kinase (WNK1) in human dorsal root ganglion Open
Inherited painless neuropathies arise due to genetic insults that either block the normal signaling of or destroy the sensory afferent neurons in the dorsal root ganglion (DRG) responsible for transducing noxious stimuli. Complete loss of …
View article: Visualizing The Spinal Coding Of Itch
Visualizing The Spinal Coding Of Itch Open
View article: Anatomical Analysis of Transient Potential Vanilloid Receptor 1 (Trpv1+) and Mu-Opioid Receptor (Oprm1+) Co-expression in Rat Dorsal Root Ganglion Neurons
Anatomical Analysis of Transient Potential Vanilloid Receptor 1 (Trpv1+) and Mu-Opioid Receptor (Oprm1+) Co-expression in Rat Dorsal Root Ganglion Neurons Open
Primary afferent neurons of the dorsal root ganglia (DRG) transduce peripheral nociceptive signals and transmit them to the spinal cord. These neurons also mediate analgesic control of the nociceptive inputs, particularly through the μ-opi…