Allan Bradley
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View article: Hematopoietic stem cell therapy with gene modification to treat sickle cell disease
Hematopoietic stem cell therapy with gene modification to treat sickle cell disease Open
Hematopoietic stem cells (HSCs) reconstitute blood cells throughout life. DNA-level correction of HSCs allows for a one-time cure of genetic diseases, including sickle cell disease (SCD). Sickle cell disease is one of the most common singl…
View article: Impact of essential genes on the success of genome editing experiments generating 3313 new genetically engineered mouse lines
Impact of essential genes on the success of genome editing experiments generating 3313 new genetically engineered mouse lines Open
The International Mouse Phenotyping Consortium (IMPC) systematically produces and phenotypes mouse lines with presumptive null mutations to provide insight into gene function. The IMPC now uses the programmable RNA-guided nuclease Cas9 for…
View article: Human-like<i>APOBEC3</i>gene expression and anti-viral responses following replacement of mouse<i>Apobec3</i>with the 7-gene human<i>APOBEC3</i>locus
Human-like<i>APOBEC3</i>gene expression and anti-viral responses following replacement of mouse<i>Apobec3</i>with the 7-gene human<i>APOBEC3</i>locus Open
The seven human APOBEC3 (hA3) genes encode polynucleotide cytidine deaminases that play vital roles in restricting replication of viruses and retrotransposons. However, off-target A3 deamination of the cellular genome is a major source of …
View article: Genetic determinants of micronucleus formation in vivo
Genetic determinants of micronucleus formation in vivo Open
Genomic instability arising from defective responses to DNA damage 1 or mitotic chromosomal imbalances 2 can lead to the sequestration of DNA in aberrant extranuclear structures called micronuclei (MN). Although MN are a hallmark of ageing…
View article: NOVEL METHODS FOR RAPID IDENTIFICATION OF BACTERIAL PATHOGENS
NOVEL METHODS FOR RAPID IDENTIFICATION OF BACTERIAL PATHOGENS Open
Intro: Antimicrobial-resistant bacteria pose a major threat to the healthcare and agricultural system. Problems with appropriate treatment in these settings arise through the lack of fast identification of the disease-causing species and l…
View article: Supplementary Table 1 from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer
Supplementary Table 1 from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer Open
Common insertion sites of piggyBac transposon mutagenesis screen.
View article: Data from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer
Data from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer Open
Biliary tract cancer ranks among the most lethal human malignancies, representing an unmet clinical need. Its abysmal prognosis is tied to an increasing incidence and a fundamental lack of mechanistic knowledge regarding the molecular basi…
View article: Supplementary Figures and Methods from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer
Supplementary Figures and Methods from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer Open
Supplementary graphical abstract, eight Supplemental Figures, Supplementary Methods
View article: Data from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer
Data from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer Open
Biliary tract cancer ranks among the most lethal human malignancies, representing an unmet clinical need. Its abysmal prognosis is tied to an increasing incidence and a fundamental lack of mechanistic knowledge regarding the molecular basi…
View article: Supplementary Table 1 from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer
Supplementary Table 1 from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer Open
Common insertion sites of piggyBac transposon mutagenesis screen.
View article: Supplementary Figures and Methods from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer
Supplementary Figures and Methods from Genetic Screens Identify a Context-Specific PI3K/p27<sup>Kip1</sup> Node Driving Extrahepatic Biliary Cancer Open
Supplementary graphical abstract, eight Supplemental Figures, Supplementary Methods
View article: <i>In vivo</i>functional genomics identifies essentiality of potassium homeostasis in medulloblastoma
<i>In vivo</i>functional genomics identifies essentiality of potassium homeostasis in medulloblastoma Open
The identification of cancer maintenance genes—driver genes essential to tumor survival—is fundamental for developing effective cancer therapy. Transposon-based insertional mutagenesis screens can identify cancer driver genes broadly but n…
View article: Development and application of a next-generation sequencing protocol and bioinformatics pipeline for the comprehensive analysis of the canine immunoglobulin repertoire
Development and application of a next-generation sequencing protocol and bioinformatics pipeline for the comprehensive analysis of the canine immunoglobulin repertoire Open
Profiling the adaptive immune repertoire using next generation sequencing (NGS) has become common in human medicine, showing promise in characterizing clonal expansion of B cell clones through analysis of B cell receptors (BCRs) in patient…
View article: Cas9-induced large deletions and small indels are controlled in a convergent fashion
Cas9-induced large deletions and small indels are controlled in a convergent fashion Open
Repair of Cas9-induced double-stranded breaks results primarily in formation of small insertions and deletions (indels), but can also cause potentially harmful large deletions. While mechanisms leading to the creation of small indels are r…
View article: A purine metabolic checkpoint that prevents autoimmunity and autoinflammation
A purine metabolic checkpoint that prevents autoimmunity and autoinflammation Open
Still's disease, the paradigm of autoinflammation-cum-autoimmunity, predisposes for a cytokine storm with excessive T lymphocyte activation upon viral infection. Loss of function of the purine nucleoside enzyme FAMIN is the sole known caus…
View article: Impact of Essential Genes on the Success of Genome Editing Experiments Generating 3,313 New Genetically Engineered Mouse Lines
Impact of Essential Genes on the Success of Genome Editing Experiments Generating 3,313 New Genetically Engineered Mouse Lines Open
The International Mouse Phenotyping Consortium (IMPC) systematically produces and phenotypes mouse lines with presumptive null mutations to provide insight into gene function. The IMPC now uses the programmable RNA-guided nuclease Cas9 for…
View article: Intronization enhances expression of S-protein and other transgenes challenged by cryptic splicing
Intronization enhances expression of S-protein and other transgenes challenged by cryptic splicing Open
The natural habitat of SARS-CoV-2 is the cytoplasm of a mammalian cell where it replicates its genome and expresses its proteins. While SARS-CoV-2 genes and hence its codons are presumably well optimized for mammalian protein translation, …
View article: <i>miR-200</i> deficiency promotes lung cancer metastasis by activating Notch signaling in cancer-associated fibroblasts
<i>miR-200</i> deficiency promotes lung cancer metastasis by activating Notch signaling in cancer-associated fibroblasts Open
Lung adenocarcinoma, the most prevalent lung cancer subtype, is characterized by its high propensity to metastasize. Despite the importance of metastasis in lung cancer mortality, its underlying cellular and molecular mechanisms remain lar…
View article: Genetic Screens Identify a Context-Specific PI3K/p27Kip1 Node Driving Extrahepatic Biliary Cancer
Genetic Screens Identify a Context-Specific PI3K/p27Kip1 Node Driving Extrahepatic Biliary Cancer Open
Biliary tract cancer ranks among the most lethal human malignancies, representing an unmet clinical need. Its abysmal prognosis is tied to an increasing incidence and a fundamental lack of mechanistic knowledge regarding the molecular basi…
View article: Gain-of-function factor H–related 5 protein impairs glomerular complement regulation resulting in kidney damage
Gain-of-function factor H–related 5 protein impairs glomerular complement regulation resulting in kidney damage Open
Significance The complement system is integral to innate immunity and host defense. However, inappropriate activation causes host tissue damage and disease. In health, this is prevented by a complex protein network that includes the factor…
View article: The Knockout Mouse Project
The Knockout Mouse Project Open
Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knock…
View article: Correction to: PiggyBac mutagenesis and exome sequencing identify genetic driver landscapes and potential therapeutic targets of EGFR-mutant gliomas
Correction to: PiggyBac mutagenesis and exome sequencing identify genetic driver landscapes and potential therapeutic targets of EGFR-mutant gliomas Open
An amendment to this paper has been published and can be accessed via the original article.
View article: Cas9-induced large deletions and small indels are controlled in a convergent fashion
Cas9-induced large deletions and small indels are controlled in a convergent fashion Open
Repair of Cas9-induced double-stranded breaks results primarily in formation of small indels, but can also cause potentially harmful large deletions. While mechanisms leading to the creation of small indels are relatively well understood, …