Amanda C. Swart
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View article: Characterization of Steroid Metabolic Pathways in Established Human and Mouse Cell Models
Characterization of Steroid Metabolic Pathways in Established Human and Mouse Cell Models Open
Immortalized adrenal, placental and gonadal cell models are often termed steroidogenic based on steroid hormone production and steroidogenic enzymes. Profiling of ‘classic’ steroid metabolites is common; however, downstream untargeted meta…
View article: The in vivo metabolism of 11-oxyandrogens and 11-oxyprogesterones: novel pathways in the steroid metabolome
The in vivo metabolism of 11-oxyandrogens and 11-oxyprogesterones: novel pathways in the steroid metabolome Open
View article: Chemopreventative potential of Salsola tuberculatiformis Botch and Compound A in the differential regulation of IL-1Ra:IL-1α in UVB exposed skin keratinocytes
Chemopreventative potential of Salsola tuberculatiformis Botch and Compound A in the differential regulation of IL-1Ra:IL-1α in UVB exposed skin keratinocytes Open
Background: Salsola tuberculatiformis Botsch, commonly known as Gannabos, is a Namibian shrub known for its contraceptive properties in rats and its ability to modulate adrenal steroidogenesis both in vitro and in vivo. Due to the labile n…
View article: Glucocorticoids and mineralocorticoids in hair: facilitating accurate diagnosis of adrenal-related endocrine disorders
Glucocorticoids and mineralocorticoids in hair: facilitating accurate diagnosis of adrenal-related endocrine disorders Open
Background Glucocorticoids and androgens in the hair follicle have been of interest for many years, particularly cortisol and cortisone in retrospective studies associated with chronic stress and Cushing’s syndrome. No studies have reporte…
View article: Circulating adrenal and gonadal steroid hormones heterogeneity in active young males and the contribution of 11-oxy androgens
Circulating adrenal and gonadal steroid hormones heterogeneity in active young males and the contribution of 11-oxy androgens Open
The classical androgens, testosterone and dihydrotestosterone, together with dehydroepiandrosterone, the precusrsor to all androgens, are generally included in diagnostic steroid evaluations of androgen excess and deficiency disorders and …
View article: C11-hydroxy and C11-oxo C19 and C21 Steroids: Pre-Receptor Regulation and Interaction with Androgen and Progesterone Steroid Receptors
C11-hydroxy and C11-oxo C19 and C21 Steroids: Pre-Receptor Regulation and Interaction with Androgen and Progesterone Steroid Receptors Open
C11-oxy C19 and C11-oxy C21 steroids have been identified as novel steroids but their function remains unclear. This study aimed to investigate the pre-receptor regulation of C11-oxy steroids by 11β-hydroxysteroid dehydrogenase (11βHSD) in…
View article: FRI382 High Androgen And Progesterone Metabolite Concentrations In Naturally Matured Follicles Are Concurrent With High Serum Anti-Müllerian Hormone Concentrations
FRI382 High Androgen And Progesterone Metabolite Concentrations In Naturally Matured Follicles Are Concurrent With High Serum Anti-Müllerian Hormone Concentrations Open
Disclosure: T. Du Toit: None. M. Von Wolff: None. A.C. Swart: None. M. Groessl: None. C.E. Flueck: None. Infertility in women is routinely evaluated with the assessment of circulating steroid levels. Profiling steroid levels in follicular …
View article: LBMON273 Not All Men Are Created Equal
LBMON273 Not All Men Are Created Equal Open
Background Clinical assessment of androgens in the diagnosis of androgen excess, hypogonadism, androgen replacement or suppressive therapy and in anti-doping practices is generally limited to a few C19 steroids. More accurate evaluations m…
View article: Androstenedione Is the Preferred Substrate for Cytochrome P450 11β-hydroxylase Leading to the Production of 11β-Hydroxyandrostenedione in the Adrenal Gland
Androstenedione Is the Preferred Substrate for Cytochrome P450 11β-hydroxylase Leading to the Production of 11β-Hydroxyandrostenedione in the Adrenal Gland Open
Adrenal cytochrome P450 11β-hydroxylase (CYP11B1) is a mitochondrial enzyme that catalyzes the final step of glucocorticoid synthesis, converting 11-deoxycortisol (S) and deoxycorticosterone (DOC) to cortisol (F) and corticosterone (CORT),…
View article: Computational modelling of the Δ4 and Δ5 adrenal steroidogenic pathways provides insight into hypocortisolism
Computational modelling of the Δ4 and Δ5 adrenal steroidogenic pathways provides insight into hypocortisolism Open
View article: Ultraperformance Convergence Chromatography Tandem Mass Spectrometry Analysis of Adrenal and Gonadal Steroid Hormones in Southern White Rhinoceros ( <i>Ceratoerium Simum Simum</i>) Faeces and Serum
Ultraperformance Convergence Chromatography Tandem Mass Spectrometry Analysis of Adrenal and Gonadal Steroid Hormones in Southern White Rhinoceros ( <i>Ceratoerium Simum Simum</i>) Faeces and Serum Open
View article: Steroid hormone analysis of adolescents and young women with polycystic ovarian syndrome and adrenocortical dysfunction using UPC2-MS/MS
Steroid hormone analysis of adolescents and young women with polycystic ovarian syndrome and adrenocortical dysfunction using UPC2-MS/MS Open
View article: SAT-742 Characterising the Metabolism, Glucuronidation and Sulfation of C11-oxy C19 Steroids
SAT-742 Characterising the Metabolism, Glucuronidation and Sulfation of C11-oxy C19 Steroids Open
The metabolism of 11β-hydroxyandrostenedione (11OHA4), a major adrenal C19 steroid, was first characterised in our in vitro prostate models showing that 11OHA4, catalysed by 11βHSDs, 17βHSDs and 5α-reductases, yields potent androgens, 11ke…
View article: The Effect of Soy Isoflavones on Steroid Metabolism
The Effect of Soy Isoflavones on Steroid Metabolism Open
Objective: This study is a post-hoc analysis of steroid hormones before and after administration of pharmacological doses of soy isoflavones in a large cohort of men and women from two independent studies. Isoflavones are rep…
View article: SAT-019 Clinical Disorders and Diseases Marked by Elevated Adrenal C11-oxy C21 and C11-oxy C19 Steroid Levels
SAT-019 Clinical Disorders and Diseases Marked by Elevated Adrenal C11-oxy C21 and C11-oxy C19 Steroid Levels Open
Adrenal steroid hormones have been implicated in numerous clinical conditions with adrenal androgens marked as major contributors in the progression of androgen-dependent diseases and disorders such as congenital adrenal hyperplasia, 21-hy…
View article: SAT-344 11α-Hydroxyprogesterone, a Potent 11β-Hydroxysteroid Dehydrgenase Inhibitor, Is Metabolised In Vitro by Adrenal Steroidogenic Enzymes
SAT-344 11α-Hydroxyprogesterone, a Potent 11β-Hydroxysteroid Dehydrgenase Inhibitor, Is Metabolised In Vitro by Adrenal Steroidogenic Enzymes Open
The C11-oxy C(21) steroids, 11αOHP4 (11α-hydroxyprogesterone) and 11βOHP4 (11β-hydroxyprogesterone), have to date been characterised only in terms of their inhibition of 11β-hydroxysteroid dehydrogenase (11βHSD). However, 11βOHP4 is also a…
View article: OR02-5 Novel Intermediates Are Catalysed By 11βHSD And CYP17A1 In The C11-oxy Backdoor Pathway Leading To The Production Of 11-ketodihydrotestosterone
OR02-5 Novel Intermediates Are Catalysed By 11βHSD And CYP17A1 In The C11-oxy Backdoor Pathway Leading To The Production Of 11-ketodihydrotestosterone Open
Adrenal C(19) steroids, testosterone and androstenedione, have long been the only androgens considered contributing to clinical conditions characterised by androgen excess. More recently, we showed that adrenal 11β-hydroxyandrostenedione i…
View article: Perspective on the regulatory role of UGT2B28 as a conjugating enzyme in the progression of prostate cancer
Perspective on the regulatory role of UGT2B28 as a conjugating enzyme in the progression of prostate cancer Open
: The maintenance of steroid homeostasis in the prostate is critical, with perturbation of steroidogenesis contributing to the modulation of active ligands in the androgen pool. In this scenario, enzymes catalysing the biosynthesis, inacti…
View article: Rooibos ( Aspalathus linearis ) facilitates an anti-inflammatory state, modulating IL-6 and IL-10 while not inhibiting the acute glucocorticoid response to a mild novel stressor in vivo
Rooibos ( Aspalathus linearis ) facilitates an anti-inflammatory state, modulating IL-6 and IL-10 while not inhibiting the acute glucocorticoid response to a mild novel stressor in vivo Open
View article: Discovery of Compound A - a selective activator of the glucocorticoid receptor with anti-inflammatory and anti-cancer activity
Discovery of Compound A - a selective activator of the glucocorticoid receptor with anti-inflammatory and anti-cancer activity Open
Glucocorticoids are among the most effective anti-inflammatory drugs, and are widely used for cancer therapy. Unfortunately, chronic treatment with glucocorticoids results in multiple side effects. Thus, there was an intensive search for s…