Amanda E. Rossiter
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View article: The Gastric Microbiota Invade the Lamina Propria in <i>Helicobacter pylori</i> ‐Associated Gastritis and Precancer
The Gastric Microbiota Invade the Lamina Propria in <i>Helicobacter pylori</i> ‐Associated Gastritis and Precancer Open
Background Stomach cancer is the fourth leading cause of cancer‐related deaths worldwide. Helicobacter pylori is the main risk factor for gastric adenocarcinoma (GAC), yet the precise mechanism underpinning this association remains controv…
View article: Open Label Vancomycin in Primary Sclerosing Cholangitis-Inflammatory Bowel Disease: Improved Colonic Disease Activity and Associations With Changes in Host–Microbiome–Metabolomic Signatures
Open Label Vancomycin in Primary Sclerosing Cholangitis-Inflammatory Bowel Disease: Improved Colonic Disease Activity and Associations With Changes in Host–Microbiome–Metabolomic Signatures Open
Background We conducted a single-arm interventional study, to explore mucosal changes associated with clinical remission under oral vancomycin (OV) treatment, in primary sclerosing cholangitis-associated inflammatory bowel disease (PSC-IBD…
View article: Noise reduction strategies in metagenomic chromosome confirmation capture to link antibiotic resistance genes to microbial hosts
Noise reduction strategies in metagenomic chromosome confirmation capture to link antibiotic resistance genes to microbial hosts Open
The gut microbiota is a reservoir for antimicrobial resistance genes (ARGs). With current sequencing methods, it is difficult to assign ARGs to their microbial hosts, particularly if these ARGs are located on plasmids. Metagenomic chromoso…
View article: Efficient Autotransporter-Mediated Extracellular Secretion of a Heterologous Recombinant Protein by Escherichia coli
Efficient Autotransporter-Mediated Extracellular Secretion of a Heterologous Recombinant Protein by Escherichia coli Open
Recombinant protein production is an essential component of the biotechnology sector. Here, we show that the autotransporter platform is a viable method for the recombinant production, secretion, and purification of a “difficult” to produc…
View article: Noise reduction strategies in metagenomic chromosome confirmation capture to link antibiotic resistance genes to microbial hosts
Noise reduction strategies in metagenomic chromosome confirmation capture to link antibiotic resistance genes to microbial hosts Open
The gut microbiota is a reservoir for antimicrobial resistance genes (ARGs). With current sequencing methods, it is difficult to assign ARGs to their microbial hosts, particularly if these ARGs are located on plasmids. Metagenomic chromoso…
View article: OP-10 Gut microbiota dysbiosis as a driver of inflammation in Ocular Mucous Membrane Pemphigoid
OP-10 Gut microbiota dysbiosis as a driver of inflammation in Ocular Mucous Membrane Pemphigoid Open
*Correspondence - Saaeha Rauz: [email protected] OBJECTIVES: Mucous Membrane Pemphigoid is an orphan multi-system autoimmune scarring disease involving mucosal sites, including the ocular surface (OcMMP) and gut. The gut microbiome plays a…
View article: Gut Dysbiosis in Ocular Mucous Membrane Pemphigoid
Gut Dysbiosis in Ocular Mucous Membrane Pemphigoid Open
Mucous Membrane Pemphigoid is an orphan multi-system autoimmune scarring disease involving mucosal sites, including the ocular surface (OcMMP) and gut. Loss of tolerance to epithelial basement membrane proteins and generation of autoreacti…
View article: Efficient autotransporter-mediated extracellular secretion of a heterologous recombinant protein by Escherichia coli
Efficient autotransporter-mediated extracellular secretion of a heterologous recombinant protein by Escherichia coli Open
Background: The autotransporter protein secretion system has previously been used to target the secretion of heterologous proteins to the bacterial cell surface and the extracellular milieu at laboratory scale. The platform is of particula…
View article: BamA and BamD Are Essential for the Secretion of Trimeric Autotransporter Adhesins
BamA and BamD Are Essential for the Secretion of Trimeric Autotransporter Adhesins Open
The BAM complex in Escherichia coli is composed of five proteins, BamA-E. BamA and BamD are essential for cell viability and are required for the assembly of β-barrel outer membrane proteins. Consequently, BamA and BamD are indispensable f…
View article: Evaluation of full-length nanopore 16S sequencing for detection of pathogens in microbial keratitis
Evaluation of full-length nanopore 16S sequencing for detection of pathogens in microbial keratitis Open
Background Microbial keratitis is a leading cause of preventable blindness worldwide. Conventional sampling and culture techniques are time-consuming, with over 40% of cases being culture-negative. Nanopore sequencing technology is portabl…
View article: Breastfeeding promotes early neonatal regulatory T‐cell expansion and immune tolerance of non‐inherited maternal antigens
Breastfeeding promotes early neonatal regulatory T‐cell expansion and immune tolerance of non‐inherited maternal antigens Open
Background Breastfeeding is associated with long‐term health benefits, such as a lower incidence of childhood infections, asthma, obesity and autoimmune disorders. However, little is known regarding how the maternal and neonatal immune sys…
View article: Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation
Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation Open
The Gram-negative outer-membrane envelops the bacterium and functions as a permeability barrier against antibiotics, detergents, and environmental stresses. Some virulence factors serve to maintain the integrity of the outer membrane, incl…
View article: Breastfeeding promotes early neonatal regulatory T cell expansion and immune tolerance of non-inherited maternal antigens
Breastfeeding promotes early neonatal regulatory T cell expansion and immune tolerance of non-inherited maternal antigens Open
Background: Breastfeeding is associated with long-term health benefits, such as a lower incidence of allergy, asthma, diabetes or celiac disease. However, little is known regarding how the maternal and neonatal immune systems interact afte…
View article: BamA and BamD are essential for the secretion of trimeric autotransporter adhesins
BamA and BamD are essential for the secretion of trimeric autotransporter adhesins Open
The BAM complex in Escherichia coli is composed of five proteins, BamA-E. BamA and BamD are essential for cell viability and are required for the assembly of β-barrel outer membrane proteins. Consequently, BamA and BamD are indispensable f…
View article: Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation
Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation Open
The Gram-negative outer membrane envelops the bacterium and functions as a permeability barrier against antibiotics, detergents and environmental stresses. Some virulence factors serve to maintain the integrity of the outer membrane, inclu…
View article: Mice Deficient in T-bet Form Inducible NO Synthase–Positive Granulomas That Fail to Constrain <i>Salmonella</i>
Mice Deficient in T-bet Form Inducible NO Synthase–Positive Granulomas That Fail to Constrain <i>Salmonella</i> Open
Clearance of intracellular infections caused by Salmonella Typhimurium (STm) requires IFN-γ and the Th1-associated transcription factor T-bet. Nevertheless, whereas IFN-γ−/− mice succumb rapidly to STm infections, T-bet−/− mice do not. In …
View article: A Pilot Integrative Analysis of Colonic Gene Expression, Gut Microbiota, and Immune Infiltration in Primary Sclerosing Cholangitis-Inflammatory Bowel Disease: Association of Disease With Bile Acid Pathways
A Pilot Integrative Analysis of Colonic Gene Expression, Gut Microbiota, and Immune Infiltration in Primary Sclerosing Cholangitis-Inflammatory Bowel Disease: Association of Disease With Bile Acid Pathways Open
Background Although a majority of patients with PSC have colitis [PSC-IBD; primary sclerosing cholangitis-inflammatory bowel disease], this is phenotypically different from ulcerative colitis [UC]. We sought to define further the pathophys…
View article: Identification of binding residues between periplasmic adapter protein (PAP) and RND efflux pumps explains PAP-pump promiscuity and roles in antimicrobial resistance
Identification of binding residues between periplasmic adapter protein (PAP) and RND efflux pumps explains PAP-pump promiscuity and roles in antimicrobial resistance Open
Active efflux due to tripartite RND efflux pumps is an important mechanism of clinically relevant antibiotic resistance in Gram-negative bacteria. These pumps are also essential for Gram-negative pathogens to cause infection and form biofi…
View article: Complete genome sequence of <i>Helicobacter pylori</i> B128 7.13 and a single‐step method for the generation of unmarked mutations
Complete genome sequence of <i>Helicobacter pylori</i> B128 7.13 and a single‐step method for the generation of unmarked mutations Open
Background Helicobacter pylori represents an interesting model of bacterial pathogenesis given that most infections are asymptomatic, while a minority of infections cause severe gastric disease. H pylori strain B128 7.13 is used extensivel…
View article: The ‘missing’ gastric microbe; the impact of gastric cancer-associated microbiota on Helicobacter pylori growth in vitro and its implications in gastric carcinogenesis
The ‘missing’ gastric microbe; the impact of gastric cancer-associated microbiota on Helicobacter pylori growth in vitro and its implications in gastric carcinogenesis Open
In recent years, multiple studies have examined the bacterial communities present in the gastric microbiota during the progression to gastric cancer (GC). Although Helicobacter pylori is the biggest risk factor for GC, the microbiota of GC…
View article: YraP Contributes to Cell Envelope Integrity and Virulence of Salmonella enterica Serovar Typhimurium
YraP Contributes to Cell Envelope Integrity and Virulence of Salmonella enterica Serovar Typhimurium Open
Mutations in σ E -regulated lipoproteins have previously been shown to impact bacterial viability under conditions of stress and during in vivo infection. YraP is conserved across a number of Gram-negative pathogens, including Neisseria me…
View article: ADTU-05 Gut microbial composition in the migrant south-asian IBD population in UK
ADTU-05 Gut microbial composition in the migrant south-asian IBD population in UK Open
Introduction Epidemiological studies have highlighted that the South Asian migrant population in UK have a comparable risk of developing IBD but with a more aggressive phenotype than the white Caucasian population. It remains unclear if th…
View article: PWE-046 TH17 cells dominate the colonic mucosal immune response in primary sclerosing cholangitis associated colitis
PWE-046 TH17 cells dominate the colonic mucosal immune response in primary sclerosing cholangitis associated colitis Open
Introduction Primary sclerosing cholangitis (PSC) is an idiopathic chronic cholestatic liver disease associated with ulcerative colitis (UC). PSC is thought to be a consequence of a genetically predisposition, dysregulated immune response …
View article: OWE-014 Whole transcriptome shotgun sequencing reveals significant upregulation of colonic mucosal immune-mediated anti-microbial mechanisms in psc-uc
OWE-014 Whole transcriptome shotgun sequencing reveals significant upregulation of colonic mucosal immune-mediated anti-microbial mechanisms in psc-uc Open
Introduction Primary sclerosing cholangitis is a hepatobiliary disorder of unknown aetiology that is commonly associated with ulcerative colitis (PSC-UC). We have previously demonstrated that patients with PSC-UC have significant alteratio…
View article: Mammalian Cell Entry domains are required for bile resistance and virulence in<i>Salmonella</i>
Mammalian Cell Entry domains are required for bile resistance and virulence in<i>Salmonella</i> Open
MCE domains were first reported in Mycobacteria as having a role in M ammalian C ell E ntry, with subsequent studies showing their importance during infection. Here, we have examined the function of MCE proteins in Salmonella Typhimurium d…
View article: P038 Th17 cells dominate the colonic mucosal immune response in primary sclerosing cholangitis associated colitis
P038 Th17 cells dominate the colonic mucosal immune response in primary sclerosing cholangitis associated colitis Open
Primary sclerosing cholangitis (PSC) is an idiopathic chronic cholestatic liver disease associated with ulcerative colitis (UC). Evidence suggests that PSC is a consequence of an interplay between a dysregulated immune response and unknown…
View article: Antigen Localization Influences the Magnitude and Kinetics of Endogenous Adaptive Immune Response to Recombinant Salmonella Vaccines
Antigen Localization Influences the Magnitude and Kinetics of Endogenous Adaptive Immune Response to Recombinant Salmonella Vaccines Open
The use of recombinant attenuated Salmonella vaccine (RASV) strains is a promising strategy for presenting heterologous antigens to the mammalian immune system to induce both cellular and humoral immune responses. However, studies on RASV …