Amanda Sales Conniff
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View article: IL-12 and PD-1 peptide combination gene therapy for the treatment of melanoma
IL-12 and PD-1 peptide combination gene therapy for the treatment of melanoma Open
View article: Physicochemical Properties of a Bioactive Polysaccharide Film from Cassia grandis with Immobilized Collagenase from Streptomyces parvulus (DPUA/1573)
Physicochemical Properties of a Bioactive Polysaccharide Film from Cassia grandis with Immobilized Collagenase from Streptomyces parvulus (DPUA/1573) Open
(1) Background: Polysaccharide films are promising vehicles for the delivery of bioactive agents such as collagenases, as they provide controlled release at the wound site, facilitating tissue regeneration. This study aimed to investigate …
View article: Pulsed Electric Fields Induce STING Palmitoylation and Polymerization Independently of Plasmid DNA Electrotransfer
Pulsed Electric Fields Induce STING Palmitoylation and Polymerization Independently of Plasmid DNA Electrotransfer Open
Gene therapy approaches may target skeletal muscle due to its high protein-expressing nature and vascularization. Intramuscular plasmid DNA (pDNA) delivery via pulsed electric fields (PEFs) can be termed electroporation or electrotransfer.…
View article: Production and biochemical and biophysical characterization of fibrinolytic protease of a Mucor subtilissimus strain isolated from the caatinga biome
Production and biochemical and biophysical characterization of fibrinolytic protease of a Mucor subtilissimus strain isolated from the caatinga biome Open
Cardiovascular diseases, resulting from the deposition of clots in blood vessels, are the leading cause of death worldwide. Fibrinolytic enzymatic activity can catalyze blood clot degradation. Findings show that 36 fungal isolates recovere…
View article: Direct Plasmid DNA Transfection into Differentiated Mouse C2C12 Myotubes
Direct Plasmid DNA Transfection into Differentiated Mouse C2C12 Myotubes Open
Background: Models of muscle cell culture allow simple manipulation to optimize gene transfer experiments, offering a significant opportunity to model muscle effects by allowing precise control of the cell culture environment in a cost-eff…
View article: DNA Electrotransfer Regulates Molecular Functions in Skeletal Muscle
DNA Electrotransfer Regulates Molecular Functions in Skeletal Muscle Open
Each muscle stimulus resulted in specific regulated molecular functions. Identifying the unique intrinsic molecular changes driven by intramuscular DNA electrotransfer will aid in the design of preventative and therapeutic gene therapies.
View article: Transcriptomic Analysis of the Acute Skeletal Muscle Effects after Intramuscular DNA Electroporation Reveals Inflammatory Signaling
Transcriptomic Analysis of the Acute Skeletal Muscle Effects after Intramuscular DNA Electroporation Reveals Inflammatory Signaling Open
Skeletal muscle is a promising tissue for therapeutic gene delivery because it is highly vascularized, accessible, and capable of synthesizing protein for therapies or vaccines. The application of electric pulses (electroporation) enhances…
View article: Acute Effects of Intratumor DNA Electrotransfer
Acute Effects of Intratumor DNA Electrotransfer Open
Intratumor therapeutic DNA electroporation or electrotransfer is in clinical trials in the United States and is under development in many other countries. Acute changes in endogenous gene expression in response to DNA or to pulse applicati…
View article: Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells
Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells Open
Germline-encoded pattern recognition receptors [PRRs] in mammalian cells function in the detection of molecular patterns associated with pathogen invasion or cellular damage. A PRR subset is activated by the atypical presence and location …
View article: In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery
In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery Open
View article: Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells
Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells Open
Germline-encoded pattern recognition receptors [PRRs] in mammalian cells function in the detection of molecular patterns associated with pathogen invasion or cellular damage. A PRR subset is activated by the atypical presence and location …
View article: A novel collagenolytic protease from Mucor subtilissimus UCP 1262: Comparative analysis of production and extraction in submerged and stated-solid fermentation
A novel collagenolytic protease from Mucor subtilissimus UCP 1262: Comparative analysis of production and extraction in submerged and stated-solid fermentation Open
This work aimed to compare the production of collagenolytic proteases produced by M. subtilissimus UCP1262 in submerged fermentation (SF) and solid-state fermentation (SSF) as well as extracting in aqueous two-phase system (ATPS). Collagen…
View article: Growth environment influences B16.F10 mouse melanoma cell response to gene electrotransfer
Growth environment influences B16.F10 mouse melanoma cell response to gene electrotransfer Open
View article: Protease from Mucor subtilissimus UCP 1262: Evaluation of several specific protease activities and purification of a fibrinolytic enzyme
Protease from Mucor subtilissimus UCP 1262: Evaluation of several specific protease activities and purification of a fibrinolytic enzyme Open
The industrial demand for proteolytic enzymes is stimulating the search for new enzyme sources. Fungal enzymes are preferred over bacterial enzymes, and more effective and easier to extract. The aim of this work was to evaluate the potenti…
View article: Protease from Mucor subtilissimus UCP 1262: Evaluation of several specific protease activities and purification of a fibrinolytic enzyme
Protease from Mucor subtilissimus UCP 1262: Evaluation of several specific protease activities and purification of a fibrinolytic enzyme Open
The industrial demand for proteolytic enzymes is stimulating the search for new enzyme sources. Fungal enzymes are preferred over bacterial enzymes, and more effective and easier to extract. The aim of this work was to evaluate the potenti…
View article: Produção e purificação integrada de protease fibrinolítica de Mucor subtilissimus UCP 1262
Produção e purificação integrada de protease fibrinolítica de Mucor subtilissimus UCP 1262 Open
RESUMO As enzimas fibrinolíticas podem ser obtidas de micro-organismos por meio de processos fermentativos. O presente trabalho teve como objetivo avaliar a produção e extração integrada da protease fibrinolítica de Mucor subtilissimus UCP…
View article: Integrated production and purification of fibrinolytic protease from Mucor subtilissimus UCP 1262
Integrated production and purification of fibrinolytic protease from Mucor subtilissimus UCP 1262 Open
Fibrinolytic enzymes can be obtained from microorganisms through fermentative processes. The study aimed to evaluate the fibrinolytic protease production and integrated extraction from Mucor subtilissimus UCP 1262 by extractive fermentatio…
View article: The neutrophil chemoattractant peptide proline-glycine-proline is associated with acute respiratory distress syndrome
The neutrophil chemoattractant peptide proline-glycine-proline is associated with acute respiratory distress syndrome Open
Acute respiratory distress syndrome (ARDS) is characterized by unrelenting polymorphonuclear neutrophil (PMN) inflammation and vascular permeability. The matrikine proline-glycine-proline (PGP) and acetylated PGP (Ac-PGP) have been shown t…
View article: Optimization of production, biochemical characterization and in vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus amyloliquefaciens
Optimization of production, biochemical characterization and in vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus amyloliquefaciens Open
The capacity of fibrinolytic enzymes to degrade blood clots makes them of high relevance in medicine and in the pharmaceutical industry. In this work, forty-three microorganisms of the genus Bacillus were evaluated for their potential to p…
View article: Simultaneous production and recovery in situ of fibrinolytic protease from Mucor subtilissimus UCP 1262
Simultaneous production and recovery in situ of fibrinolytic protease from Mucor subtilissimus UCP 1262 Open
View article: Purification of a fibrinolytic protease from Mucor subtilissimus UCP 1262 by aqueous two-phase systems (PEG/sulfate)
Purification of a fibrinolytic protease from Mucor subtilissimus UCP 1262 by aqueous two-phase systems (PEG/sulfate) Open
A fibrinolytic protease from M. subtilissimus UCP 1262 was recovered and partially purified by polyethylene glycol (PEG)/sodium sulfate aqueous two-phase systems (ATPS). The simultaneous influence of PEG molar mass, PEG concentration and s…
View article: Protease fibrinolítica de Mucor subtilissimus UCP 1262 : produção, purificação, caracterização bioquímica e estrutural
Protease fibrinolítica de Mucor subtilissimus UCP 1262 : produção, purificação, caracterização bioquímica e estrutural Open
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View article: PURIFICATION AND CHARACTERIZATION OF A NOVEL PROTEASE WITH FIBRINOLYTIC ACTIVITY FROM Mucor subtilissimus UCP 1262
PURIFICATION AND CHARACTERIZATION OF A NOVEL PROTEASE WITH FIBRINOLYTIC ACTIVITY FROM Mucor subtilissimus UCP 1262 Open
A novel protease with fibrinolytic activity was purified from MucorsubtilissimusUCP 1262usinga two-step purification protocol.The enzyme was pre-purified using cetonic precipitation and adsorbed by ion exchange chromatography on DEAE-sepha…
View article: Production and Characterization of New Fibrinolytic Protease from <i>Mucor subtillissimus</i> UCP 1262 in Solid-State Fermentation
Production and Characterization of New Fibrinolytic Protease from <i>Mucor subtillissimus</i> UCP 1262 in Solid-State Fermentation Open
Fibrinolytic enzymes have received attention regarding their medicinal potential for thrombolytic diseases, a leading cause of morbidity and mortality worldwide. Various natural enzymes purified from animal, plant and microbial sources hav…