Amanda Sproule
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View article: Chemodiversity of Penicillium isolated from alpine and arctic environments, including ten new species
Chemodiversity of Penicillium isolated from alpine and arctic environments, including ten new species Open
Polar, high altitude montane and cold desert environments harbour only sparse plant life and often remain frozen for extended periods. Because of their remoteness, often combined with restricted access, such regions are rarely visited and …
View article: Identification and Confirmation of Virulence Factor Production from Fusarium avenaceum, a Causal Agent of Root Rot in Pulses
Identification and Confirmation of Virulence Factor Production from Fusarium avenaceum, a Causal Agent of Root Rot in Pulses Open
Fusarium avenaceum is an aggressive pathogen of pulse crops and a causal agent in root rot disease that negatively impacts Canadian agriculture. This study reports the results of a targeted metabolomics-based profiling of secondary metabol…
View article: Bacterial growth‐mediated systems remodelling of <i>Nicotiana benthamiana</i> defines unique signatures of target protein production in molecular pharming
Bacterial growth‐mediated systems remodelling of <i>Nicotiana benthamiana</i> defines unique signatures of target protein production in molecular pharming Open
Summary The need for therapeutics to treat a plethora of medical conditions and diseases is on the rise and the demand for alternative approaches to mammalian‐based production systems is increasing. Plant‐based strategies provide a safe an…
View article: Two novel Pleosporales species isolated from the bark of Acer saccharum
Two novel Pleosporales species isolated from the bark of Acer saccharum Open
During a survey of culturable microfungi from the bark of sugar maple (Acer saccharum), Atrocalyx glutinosus and Nigrograna rubescens, two novel species of Pleosporales (Dothideomycetes) were isolated from several locations in eastern Onta…
View article: Epistatic Relationship between MGV1 and TRI6 in the Regulation of Biosynthetic Gene Clusters in Fusarium graminearum
Epistatic Relationship between MGV1 and TRI6 in the Regulation of Biosynthetic Gene Clusters in Fusarium graminearum Open
Genetic studies have shown that the MAP kinase MGV1 and the transcriptional regulator TRI6 regulate many of the same biosynthetic gene clusters (BGCs) in Fusarium graminearum. This study sought to investigate the relationship between MGV1 …
View article: Multiple Clonostachys rosea UDP-Glycosyltransferases Contribute to the Production of 15-Acetyl-Deoxynivalenol-3-O-Glycoside When Confronted with Fusarium graminearum
Multiple Clonostachys rosea UDP-Glycosyltransferases Contribute to the Production of 15-Acetyl-Deoxynivalenol-3-O-Glycoside When Confronted with Fusarium graminearum Open
Mycotoxins, derived from toxigenic fungi such as Fusarium, Aspergillus, and Penicillium species have impacted the human food chain for thousands of years. Deoxynivalenol (DON), is a tetracyclic sesquiterpenoid type B trichothecene mycotoxi…
View article: CRISPR-Cas9 Gene Editing and Secondary Metabolite Screening Confirm Fusarium graminearum C16 Biosynthetic Gene Cluster Products as Decalin-Containing Diterpenoid Pyrones
CRISPR-Cas9 Gene Editing and Secondary Metabolite Screening Confirm Fusarium graminearum C16 Biosynthetic Gene Cluster Products as Decalin-Containing Diterpenoid Pyrones Open
Fusarium graminearum is a causal organism of Fusarium head blight in cereals and maize. Although a few secondary metabolites produced by F. graminearum are considered disease virulence factors, many molecular products of biosynthetic gene …
View article: Untargeted metabolomics screening reveals unique secondary metabolite production from Alternaria section Alternaria
Untargeted metabolomics screening reveals unique secondary metabolite production from Alternaria section Alternaria Open
Alternaria section Alternaria is comprised of many species that infect a broad diversity of important crop plants and cause post-harvest spoilage. Alternaria section Alternaria species, such as A. alternata and A. arborescens , are prolifi…
View article: CRISPR/Cas9 disruption of <i>UGT71L1</i> in poplar connects salicinoid and salicylic acid metabolism and alters growth and morphology
CRISPR/Cas9 disruption of <i>UGT71L1</i> in poplar connects salicinoid and salicylic acid metabolism and alters growth and morphology Open
Salicinoids are salicyl alcohol-containing phenolic glycosides with strong antiherbivore effects found only in poplars and willows. Their biosynthesis is poorly understood, but recently a UDP-dependent glycosyltransferase, UGT71L1, was sho…
View article: Exploring Within-Ecodistrict Lake Organic Matter Variability and Identifying Possible Environmental Contaminant Biomarkers Using Sedimentomics
Exploring Within-Ecodistrict Lake Organic Matter Variability and Identifying Possible Environmental Contaminant Biomarkers Using Sedimentomics Open
View article: Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia
Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia Open
Research into ergot alkaloid production in major cereal cash crops is crucial for furthering our understanding of the potential toxicological impacts of Claviceps purpurea upon Canadian agriculture and to ensure consumer safety. An untarge…
View article: A metabolomic study of vegetative incompatibility in Cryphonectria parasitica
A metabolomic study of vegetative incompatibility in Cryphonectria parasitica Open
Vegetative incompatibility (VI) is a form of non-self allorecognition in filamentous fungi that restricts conspecific hyphal fusion and the formation of heterokaryons. In the chestnut pathogenic fungus, Cryphonectria parasitica, VI is cont…
View article: Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates
Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates Open
View article: DNA Methylation Is Responsive to the Environment and Regulates the Expression of Biosynthetic Gene Clusters, Metabolite Production, and Virulence in Fusarium graminearum
DNA Methylation Is Responsive to the Environment and Regulates the Expression of Biosynthetic Gene Clusters, Metabolite Production, and Virulence in Fusarium graminearum Open
Histone modifications play a significant role in the regulation of biosynthetic gene clusters (BGCs) in the phytopathogen Fusarium graminearum , by contrast, epigenetic regulation by DNA methyltransferases (DNMTs) is less documented. In th…
View article: Additional file 7 of Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates
Additional file 7 of Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates Open
Additional file 7. Matrix of averaged chemical phenotype data (all mass features included in analysis). Values represent detection frequencies averaged from 5 media conditions. Column headings (mass features) are in RT_m/z format, where RT…
View article: Additional file 1 of Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates
Additional file 1 of Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates Open
Additional file 1 Summary of all F. poae isolates screened in this study. Data includes DAOMC accession numbers, crop pathology codes, locations, country of origin, host crop type, groupings inferred by analysis of TEF1-α, TRI1 and TRI8 ge…
View article: Additional file 17 of Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates
Additional file 17 of Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates Open
Additional file 17. Compressed folder containing alignments for each of the 4141 orthologs used in BUSCO analysis.
View article: Apicidin Biosynthesis Is Linked to Accessory Chromosomes in Fusarium Poae Isolates
Apicidin Biosynthesis Is Linked to Accessory Chromosomes in Fusarium Poae Isolates Open
Background: Fusarium poae is frequently associated with cereal crops showing symptoms of Fusarium head blight, a disease of global concern that reduces crop yields and renders grains unfit for consumption due to mycotoxin contamination. Wh…
View article: Activation of biosynthetic gene clusters by the global transcriptional regulator <i>TRI6</i> in <i>Fusarium graminearum</i>
Activation of biosynthetic gene clusters by the global transcriptional regulator <i>TRI6</i> in <i>Fusarium graminearum</i> Open
In F. graminearum , the transcription factor TRI6 positively regulates the trichothecene biosynthetic gene cluster (BGC) leading to the production of the secondary metabolite 15‐acetyl deoxynivalenol. Secondary metabolites are not essentia…
View article: Transcriptomic and Exometabolomic Profiling Reveals Antagonistic and Defensive Modes of<i>Clonostachys rosea</i>Action Against<i>Fusarium graminearum</i>
Transcriptomic and Exometabolomic Profiling Reveals Antagonistic and Defensive Modes of<i>Clonostachys rosea</i>Action Against<i>Fusarium graminearum</i> Open
The mycoparasite Clonostachys rosea ACM941 is under development as a biocontrol organism against Fusarium graminearum, the causative agent of Fusarium head blight in cereals. To identify molecular factors associated with this interaction, …
View article: Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas
Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas Open
Fusarium avenaceum is a generalist pathogen responsible for diseases in numerous crop species. The fungus produces a series of mycotoxins including the cyclohexadepsipeptide enniatins. Mycotoxins can be pathogenicity and virulence factors …
View article: Four phylogenetic species of ergot from Canada and their characteristics in morphology, alkaloid production, and pathogenicity
Four phylogenetic species of ergot from Canada and their characteristics in morphology, alkaloid production, and pathogenicity Open
Four ergot species (Claviceps ripicola, C. quebecensis, C. perihumidiphila, and C. occidentalis) were recognized based on analyses of DNA sequences from multiple loci, including two housekeeping genes, RNA polymerase II secon…
View article: Terrosamycins A and B, Bioactive Polyether Ionophores from Streptomyces sp. RKND004 from Prince Edward Island Sediment
Terrosamycins A and B, Bioactive Polyether Ionophores from Streptomyces sp. RKND004 from Prince Edward Island Sediment Open
Terrosamycins A (1) and B (2), two polycyclic polyether natural products, were purified from the fermentation broth of Streptomyces sp. RKND004 isolated from Prince Edward Island sediment. The one strain-many compounds (OSMAC) approach cou…
View article: Whole Genome Sequencing and Metabolomic Study of Cave Streptomyces Isolates ICC1 and ICC4
Whole Genome Sequencing and Metabolomic Study of Cave Streptomyces Isolates ICC1 and ICC4 Open
The terrestrial subsurface microbiome has gained considerable amount of interests in the recent years because of its rich potential resource for biomining novel genes coding for metabolites possessing antimicrobial activities. In our previ…
View article: Discovery of a New Natural Product and a Deactivation of a Quorum Sensing System by Culturing a “Producer” Bacterium With a Heat-Killed “Inducer” Culture
Discovery of a New Natural Product and a Deactivation of a Quorum Sensing System by Culturing a “Producer” Bacterium With a Heat-Killed “Inducer” Culture Open
Herein we describe a modified bacterial culture methodology as a tool to discover new natural products via supplementing actinomycete fermentation media with autoclaved cultures of "inducer" microbes. Using seven actinomycetes and four ind…