Amiq Gazdhar
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View article: Adoptive Transfer of T Cells as a Potential Therapeutic Approach in the Bleomycin‐Injured Mouse Lung
Adoptive Transfer of T Cells as a Potential Therapeutic Approach in the Bleomycin‐Injured Mouse Lung Open
Background Idiopathic pulmonary fibrosis (IPF) is a lethal disease with an unknown etiology and complex pathophysiology that are not fully understood. The disease involves intricate cellular interplay, particularly among various immune cel…
View article: Matrix metalloproteinase 24 (MMP24) is a pro-fibrotic mediator in lung fibrosis
Matrix metalloproteinase 24 (MMP24) is a pro-fibrotic mediator in lung fibrosis Open
Idiopathic pulmonary fibrosis (IPF) represents a terminal, age-related disease characterized by a complex pathophysiology. Recent studies have reported the presence of basal cells in IPF lungs, yet their specific role and impact on other c…
View article: Biogenic polymer-based patches for congenital cardiac surgery: a feasibility study
Biogenic polymer-based patches for congenital cardiac surgery: a feasibility study Open
Objective Currently used patch materials in congenital cardiac surgery do not grow, renew, or remodel. Patch calcification occurs more rapidly in pediatric patients eventually leading to reoperations. Bacterial cellulose (BC) as a biogenic…
View article: Anti-Fibrotic Effect of SDF-1β Overexpression in Bleomycin-Injured Rat Lung
Anti-Fibrotic Effect of SDF-1β Overexpression in Bleomycin-Injured Rat Lung Open
Rational: Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease and is associated with high mortality due to a lack of effective treatment. Excessive deposition of the extracellular matrix by activated myofibroblas…
View article: Basal-Like Cell-Conditioned Medium Exerts Anti-Fibrotic Effects In Vitro and In Vivo
Basal-Like Cell-Conditioned Medium Exerts Anti-Fibrotic Effects In Vitro and In Vivo Open
In idiopathic pulmonary fibrosis (IPF), basal-like cells are atypically present in the alveolar region, where they may affect adjacent stromal cells by paracrine mechanisms. We here aimed to confirm the presence of basal-like cells in peri…
View article: LRR-protein RNH1 dampens the inflammasome activation and is associated with COVID-19 severity
LRR-protein RNH1 dampens the inflammasome activation and is associated with COVID-19 severity Open
Inflammasomes are cytosolic innate immune sensors of pathogen infection and cellular damage that induce caspase-1–mediated inflammation upon activation. Although inflammation is protective, uncontrolled excessive inflammation can cause inf…
View article: Electrospray Mediated Localized and Targeted Chemotherapy in a Mouse Model of Lung Cancer
Electrospray Mediated Localized and Targeted Chemotherapy in a Mouse Model of Lung Cancer Open
Background: An advanced stage, centrally localized invasive tumor is a major cause of sudden death in lung cancer patients. Currently, chemotherapy, radiotherapy, laser ablation, or surgical resection if possible are the available state-of…
View article: LRR protein RNH1 dampens the inflammasome activation and is associated with adverse clinical outcomes in COVID-19 patients
LRR protein RNH1 dampens the inflammasome activation and is associated with adverse clinical outcomes in COVID-19 patients Open
Inflammasomes are cytosolic innate immune sensors of pathogen infection and cellular damage that induce caspase-1 mediated inflammation upon activation. Although inflammation is protective, uncontrolled excessive inflammation can cause inf…
View article: Efficacy of Combined in-vivo Electroporation-Mediated Gene Transfer of VEGF, HGF, and IL-10 on Skin Flap Survival, Monitored by Label-Free Optical Imaging: A Feasibility Study
Efficacy of Combined in-vivo Electroporation-Mediated Gene Transfer of VEGF, HGF, and IL-10 on Skin Flap Survival, Monitored by Label-Free Optical Imaging: A Feasibility Study Open
Preventing surgical flaps necrosis remains challenging. Laser Doppler imaging and ultrasound can monitor blood flow in flap regions, but they do not directly measure the cellular response to ischemia. The study aimed to investigate the eff…
View article: Enhancing CRISPR deletion via pharmacological delay of DNA-PKcs
Enhancing CRISPR deletion via pharmacological delay of DNA-PKcs Open
CRISPR-Cas9 deletion (CRISPR-del) is the leading approach for eliminating DNA from mammalian cells and underpins a variety of genome-editing applications. Target DNA, defined by a pair of double-strand breaks (DSBs), is removed during nonh…
View article: Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro
Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro Open
Induced pluripotent stem cell secretome (iPSC-CM) mitigate organ injury and help in repair. Macrophages play a critical role in tissue repair and regeneration and can be directed to promote tissue repair by iPSC-CM, although the exact mech…
View article: Non-viral Gene Delivery Methods for Bone and Joints
Non-viral Gene Delivery Methods for Bone and Joints Open
Viral carrier transport efficiency of gene delivery is high, depending on the type of vector. However, viral delivery poses significant safety concerns such as inefficient/unpredictable reprogramming outcomes, genomic integration, as well …
View article: Inhalational delivery of induced pluripotent stem cell secretome improves postpneumonectomy lung structure and function
Inhalational delivery of induced pluripotent stem cell secretome improves postpneumonectomy lung structure and function Open
To examine whether the secreted products of human induced pluripotent stem cells (iPSCs) facilitate innate adaptive responses following loss of lung tissue, adult dogs underwent surgical removal of one lung, then received repeated administ…
View article: In Vivo Electroporation-Mediated, Intrahepatic Alpha1 Antitrypsin Gene Transfer Reduces Pulmonary Emphysema in Pallid Mice
In Vivo Electroporation-Mediated, Intrahepatic Alpha1 Antitrypsin Gene Transfer Reduces Pulmonary Emphysema in Pallid Mice Open
Rationale: Mutation in the alpha1 antitrypsin (AAT) gene leads to low circulating levels of AAT, which is associated with several disease processes including pulmonary emphysema. The standard of care relies on substitution with plasma-puri…
View article: In vitro culture of aberrant basal-like cells from fibrotic lung tissue
In vitro culture of aberrant basal-like cells from fibrotic lung tissue Open
Rationale In idiopathic pulmonary fibrosis (IPF) atypical epithelial cells are present in the alveolar compartment. Their origin and contribution to IPF pathogenesis is unknown. We recently cultured a distinct population of cells, which re…
View article: Additional file 1 of Transcriptomic profiling reveals disease-specific characteristics of epithelial cells in idiopathic pulmonary fibrosis
Additional file 1 of Transcriptomic profiling reveals disease-specific characteristics of epithelial cells in idiopathic pulmonary fibrosis Open
Additional file 1: Supplementary Table 1. List of Differentially Expressed Genes. A total of 199 genes were differentially expressed in MSC-like cell-derived ECs from IPF versus non-IPF patients, with 80 genes upregulated and 119 genes dow…
View article: Mitochondrial DNA mutations and respiratory chain dysfunction in idiopathic and connective tissue disease-related lung fibrosis
Mitochondrial DNA mutations and respiratory chain dysfunction in idiopathic and connective tissue disease-related lung fibrosis Open
Reactive oxygen species (ROS) are implicated in the aetiology of interstitial lung disease (ILD). We investigated the role of large-scale somatically acquired mutations in mitochondrial DNA (mtDNA) and consecutive respiratory chain dysfunc…
View article: Stem cell secretome attenuates acute rejection in rat lung allotransplant
Stem cell secretome attenuates acute rejection in rat lung allotransplant Open
The BMSC-sec is a promising novel cell-based therapeutic option for acute rejection in a rat lung allograft model.
View article: Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells
Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells Open
Differentiation of primary alveolar type II epithelial cells (AEC II) to AEC type I in culture is a major barrier in the study of the alveolar epithelium in vitro. The establishment of an AEC II cell line derived from induced pluripotent s…
View article: Application of electrochemotherapy in the management of primary and metastatic cutaneous malignant tumours: a systematic review and meta-analysis
Application of electrochemotherapy in the management of primary and metastatic cutaneous malignant tumours: a systematic review and meta-analysis Open
Electrochemotherapy is becoming a promising technique for the management of malignancies of skin and non-skin origin. The current review aims to clarify current knowledge on administration of electrochemotherapy for the treatment of variou…
View article: A new electrospray method for targeted gene delivery
A new electrospray method for targeted gene delivery Open
A challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray…
View article: Alpha-Klotho Enrichment in Induced Pluripotent Stem Cell Secretome Contributes to Antioxidative Protection in Acute Lung Injury
Alpha-Klotho Enrichment in Induced Pluripotent Stem Cell Secretome Contributes to Antioxidative Protection in Acute Lung Injury Open
Induced pluripotent stem cells (iPSCs) have been reported to alleviate organ injury, although the mechanisms of action remain unclear and administration of intact cells faces many limitations. We hypothesized that cell-free conditioned med…