Amit A. Lugade
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View article: mTOR inhibition modulates vaccine-induced immune responses to generate memory T cells in patients with solid tumors
mTOR inhibition modulates vaccine-induced immune responses to generate memory T cells in patients with solid tumors Open
Background Perturbation of the mechanistic target of rapamycin (mTOR) pathway can instruct effector versus memory cell fate of tumor antigen-specific T cells in preclinical models. In this study, we sought to understand the impact of rapam…
View article: Supplementary Figure 2 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 2 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 115K, Supplementary Figure 2. Impact of chronic viral infection or pretreatment of HCC patients on Tregs.
View article: Supplementary Figure 1 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 1 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 81K, Supplementary Figure 1. (A) q-PCR analysis of Foxp3 gene expression from PBMCs of HCC patients (n=10) and healthy donors (n=10). Lines represent mean values of Foxp3 mRNA copies normalized to GAPDH control (**P = 0.005). (B…
View article: Supplementary Figure 1 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 1 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 81K, Supplementary Figure 1. (A) q-PCR analysis of Foxp3 gene expression from PBMCs of HCC patients (n=10) and healthy donors (n=10). Lines represent mean values of Foxp3 mRNA copies normalized to GAPDH control (**P = 0.005). (B…
View article: Supplementary Methods from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Methods from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 7K
View article: Supplementary Figure 5 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 5 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 117K, Supplementary Figure 5. Plasmacytoid dendritic cells in HCC patients.
View article: Supplementary Figure 3 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 3 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 175K, Supplementary Figure 3. Effect of chronic viral infection or pre-treatment of HCC patients on GARP and CTL-A4 expression.
View article: Supplementary Figure 6 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 6 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 139K, Supplementary Figure 6. Intracellular cytokine expression from in vitro stimulated T cells.
View article: Supplementary Methods from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Methods from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 7K
View article: Supplementary Figure 3 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 3 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 175K, Supplementary Figure 3. Effect of chronic viral infection or pre-treatment of HCC patients on GARP and CTL-A4 expression.
View article: Supplementary Figure 4 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 4 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 117K, Supplementary Figure 4. (A, B) Frequency as well as (C, D) absolute number of MDSC in the peripheral blood of HCC patients was compared after stratification of patients based on exposure to HCV infection or prior treatment.
View article: Supplementary Figure 5 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 5 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 117K, Supplementary Figure 5. Plasmacytoid dendritic cells in HCC patients.
View article: Supplementary Figure 4 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 4 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 117K, Supplementary Figure 4. (A, B) Frequency as well as (C, D) absolute number of MDSC in the peripheral blood of HCC patients was compared after stratification of patients based on exposure to HCV infection or prior treatment.
View article: Supplementary Figure 6 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 6 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 139K, Supplementary Figure 6. Intracellular cytokine expression from in vitro stimulated T cells.
View article: Data from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Data from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
The extent to which T-cell–mediated immune surveillance is impaired in human cancer remains a question of major importance, given its potential impact on the development of generalized treatments of advanced disease where the highest degre…
View article: Supplementary Figure 2 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Supplementary Figure 2 from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
PDF file - 115K, Supplementary Figure 2. Impact of chronic viral infection or pretreatment of HCC patients on Tregs.
View article: Data from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality
Data from Higher Frequencies of GARP<sup>+</sup>CTLA-4<sup>+</sup>Foxp3<sup>+</sup> T Regulatory Cells and Myeloid-Derived Suppressor Cells in Hepatocellular Carcinoma Patients Are Associated with Impaired T-Cell Functionality Open
The extent to which T-cell–mediated immune surveillance is impaired in human cancer remains a question of major importance, given its potential impact on the development of generalized treatments of advanced disease where the highest degre…
View article: Carboplatin enhances lymphocyte-endothelial interactions to promote CD8+ T cell trafficking into the ovarian tumor microenvironment
Carboplatin enhances lymphocyte-endothelial interactions to promote CD8+ T cell trafficking into the ovarian tumor microenvironment Open
View article: Whole-Genome Sequencing Identifies PPARGC1A as a Putative Modifier of Cancer Risk in BRCA1/2 Mutation Carriers
Whole-Genome Sequencing Identifies PPARGC1A as a Putative Modifier of Cancer Risk in BRCA1/2 Mutation Carriers Open
While BRCA1 and BRCA2 mutations are known to confer the largest risk of breast cancer and ovarian cancer, the incomplete penetrance of the mutations and the substantial variability in age at cancer onset among carriers suggest additional f…
View article: Metabolic adaptation of ovarian tumors in patients treated with an IDO1 inhibitor constrains antitumor immune responses
Metabolic adaptation of ovarian tumors in patients treated with an IDO1 inhibitor constrains antitumor immune responses Open
To uncover underlying mechanisms associated with failure of indoleamine 2,3-dioxygenase 1 (IDO1) blockade in clinical trials, we conducted a pilot, window-of-opportunity clinical study in 17 patients with newly diagnosed advanced high-grad…
View article: A prime/boost vaccine platform efficiently identifies CD27 agonism and depletion of myeloid-derived suppressor cells as therapies that rationally combine with checkpoint blockade in ovarian cancer
A prime/boost vaccine platform efficiently identifies CD27 agonism and depletion of myeloid-derived suppressor cells as therapies that rationally combine with checkpoint blockade in ovarian cancer Open
View article: IDO1 Expression in Ovarian Cancer Induces PD-1 in T Cells via Aryl Hydrocarbon Receptor Activation
IDO1 Expression in Ovarian Cancer Induces PD-1 in T Cells via Aryl Hydrocarbon Receptor Activation Open
The immunoregulatory enzyme, indoleamine 2,3-dioxygenase (IDO1) and the PD-1/PD-L1 axis are potent mechanisms that impede effective anti-tumor immunity in ovarian cancer. However, whether the IDO pathway regulates PD-1 expression in T cell…
View article: Indoleamine 2,3-dioxygenase upregulates PD-1 expression on ovarian tumor infiltrating CD8<sup>+</sup> T cells via kynurenine activation of the aryl hydrocarbon receptor
Indoleamine 2,3-dioxygenase upregulates PD-1 expression on ovarian tumor infiltrating CD8<sup>+</sup> T cells via kynurenine activation of the aryl hydrocarbon receptor Open
The immunoregulatory enzyme, indoleamine 2,3-dioxygenase (IDO1) and the PD-1/PD-L1 axis are potent mechanisms that impede effective anti-tumor immunity in ovarian cancer. However, whether the IDO pathway regulates PD-1 expression in T cell…
View article: WHSC1/NSD2 regulates immune infiltration in prostate cancer
WHSC1/NSD2 regulates immune infiltration in prostate cancer Open
Background Immunotherapy in prostate cancer (PCa) lags behind the progresses obtained in other cancer types partially because of its limited immune infiltration. Tumor-resident immune cells have been detected in the prostate, but the regul…
View article: Key Parameters of Tumor Epitope Immunogenicity Revealed Through a Consortium Approach Improve Neoantigen Prediction
Key Parameters of Tumor Epitope Immunogenicity Revealed Through a Consortium Approach Improve Neoantigen Prediction Open
View article: Circulating CD14<sup>+</sup>HLA‐DR<sup>lo/−</sup> monocytic cells as a biomarker for epithelial ovarian cancer progression
Circulating CD14<sup>+</sup>HLA‐DR<sup>lo/−</sup> monocytic cells as a biomarker for epithelial ovarian cancer progression Open
Problem Previous studies identified circulating CD14 + HLA‐DR lo/− monocytic cells as an immune suppressive subset in solid malignancies, such as prostate, renal cell carcinoma, and pancreatic cancer. Such monocytic cells have been implica…
View article: Clonality and antigen-specific responses shape the prognostic effects of tumor-infiltrating T cells in ovarian cancer
Clonality and antigen-specific responses shape the prognostic effects of tumor-infiltrating T cells in ovarian cancer Open
CD8+ tumor-infiltrating lymphocytes (TILs) are not all specific for tumor antigens, but can include bystander TILs that are specific for cancer-irrelevant epitopes, and it is unknown whether the T-cell repertoire affects prognos…
View article: Immunotherapy for Gynecologic Cancer: Current Applications and Future Directions
Immunotherapy for Gynecologic Cancer: Current Applications and Future Directions Open
The role of the immune system in the development of cancer has been a subject of ongoing clinical investigation in recent years. Emerging data demonstrate that tumorigenesis resulting in ovarian, uterine, and cervical cancers is a conseque…
View article: Oncolytic Maraba virus armed with tumor antigen boosts vaccine priming and reveals diverse therapeutic response patterns when combined with checkpoint blockade in ovarian cancer
Oncolytic Maraba virus armed with tumor antigen boosts vaccine priming and reveals diverse therapeutic response patterns when combined with checkpoint blockade in ovarian cancer Open
Our findings point to a key hierarchical role for PD-1 signaling and adaptive immune resistance in the ovarian TME in determining the functional fate of tumor-specific CD8+ T cells, even in the context of robust therapy mediated…
View article: Whole‐exome sequencing of ovarian cancer families uncovers putative predisposition genes
Whole‐exome sequencing of ovarian cancer families uncovers putative predisposition genes Open
Despite the identification of several ovarian cancer (OC) predisposition genes, a large proportion of familial OC risk remains unexplained. We adopted a two‐stage design to identify new OC predisposition genes. We first carried out a large…