Ana Gimeno
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View article: Non-Carbohydrate Inhibitors of Sialic Acid-binding Immunomodulatory-type Lectin-7 (Siglec-7) Discovered from Genetically Encoded Bicyclic Peptide Libraries
Non-Carbohydrate Inhibitors of Sialic Acid-binding Immunomodulatory-type Lectin-7 (Siglec-7) Discovered from Genetically Encoded Bicyclic Peptide Libraries Open
Glycan-binding proteins (GBP) are among the most difficult to drug targets. This deficiency delays clinical progress for therapeutically important GBPs. We employed bicyclic genetically encoded libraries (BiGELs), produced by chemical modi…
View article: Unravelling structure–function interactions between fluorinated heparan sulfate mimetics and signaling proteins
Unravelling structure–function interactions between fluorinated heparan sulfate mimetics and signaling proteins Open
Fluorinated carbohydrates are emerging scaffolds in glycobiology, enabling the elucidation of the roles of the individual hydroxyl groups of a carbohydrate in protein binding and drug discovery.
View article: Decoding the Molecular Basis of the Specificity of an Anti-sTn Antibody
Decoding the Molecular Basis of the Specificity of an Anti-sTn Antibody Open
The mucin O-glycan sialyl Tn antigen (sTn, Neu5Acα2-6GalNAcα1-O-Ser/Thr) is an antigen associated with different types of cancers, often linked with a higher risk of metastasis and poor prognosis. Despite efforts to develop a…
View article: Site-Specific Glyco-Tagging of Native Proteins for the Development of Biologicals
Site-Specific Glyco-Tagging of Native Proteins for the Development of Biologicals Open
Glycosylation is an attractive approach to enhance biological properties of pharmaceutical proteins; however, the precise installation of glycans for structure-function studies remains challenging. Here, we describe a chemoenzymatic method…
View article: Unveiling the <scp>GT114</scp> family: Structural characterization of <scp>A075L</scp>, a glycosyltransferase from <i>Paramecium bursaria</i> chlorella virus‐1 (<scp>PBCV</scp>‐1)
Unveiling the <span>GT114</span> family: Structural characterization of <span>A075L</span>, a glycosyltransferase from <i>Paramecium bursaria</i> chlorella virus‐1 (<span>PBCV</span>‐1) Open
Protein A075L is a β‐xylosyltransferase that participates in producing the core of the N‐glycans found in VP54, the major viral capsid protein of Paramecium bursaria chlorella virus‐1 (PBCV‐1). In this study, we present an X‐ray crystallog…
View article: A Chemoenzymatic Strategy for Site-Specific Glyco-Tagging of Native Proteins for the Development of Biologicals
A Chemoenzymatic Strategy for Site-Specific Glyco-Tagging of Native Proteins for the Development of Biologicals Open
Glycosylation is an attractive approach to enhance biological properties of pharmaceutical proteins, however, precise installation of glycans for structure-function studies remains challenging. Here, we describe a chemoenzymatic methodolog…
View article: Exploring galectin interactions with human milk oligosaccharides and blood group antigens identifies BGA6 as a functional galectin-4 ligand
Exploring galectin interactions with human milk oligosaccharides and blood group antigens identifies BGA6 as a functional galectin-4 ligand Open
Galectins (Gals), a family of multifunctional glycan-binding proteins, have been traditionally defined as β-galactoside binding lectins. However, certain members of this family have shown selective affinity toward specific glycan structure…
View article: Solid‐Phase‐Supported Chemoenzymatic Synthesis and Analysis of Chondroitin Sulfate Proteoglycan Glycopeptides
Solid‐Phase‐Supported Chemoenzymatic Synthesis and Analysis of Chondroitin Sulfate Proteoglycan Glycopeptides Open
Proteoglycans (PGs), consisting of glycosaminoglycans (GAGs) linked with the core protein through a tetrasaccharide linkage region, play roles in many important biological events. The chemical synthesis of PG glycopeptides is extremely cha…
View article: Solid‐Phase‐Supported Chemoenzymatic Synthesis and Analysis of Chondroitin Sulfate Proteoglycan Glycopeptides
Solid‐Phase‐Supported Chemoenzymatic Synthesis and Analysis of Chondroitin Sulfate Proteoglycan Glycopeptides Open
Proteoglycans (PGs), consisting of glycosaminoglycans (GAGs) linked with the core protein through a tetrasaccharide linkage region, play roles in many important biological events. The chemical synthesis of PG glycopeptides is extremely cha…
View article: Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue
Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue Open
The interactions of glycans with proteins modulate many events related to health and disease. In fact, the establishment of these recognition events and their biological consequences are intimately related to the three-dimensional structur…
View article: Unprecedented selectivity for homologous lectin targets: differential targeting of the viral receptors L-SIGN and DC-SIGN
Unprecedented selectivity for homologous lectin targets: differential targeting of the viral receptors L-SIGN and DC-SIGN Open
DC-SIGN (CD209) and L-SIGN (CD209L) are two C-type lectin receptors (CLRs) that facilitate SARS-CoV-2 infections as viral co-receptors. SARS-CoV-2 manipulates both DC-SIGN and L-SIGN for enhanced infection, leading to interest in developin…
View article: Unprecedented selectivity for homologous lectin targets: differential targeting of the viral receptors L-SIGN and DC-SIGN
Unprecedented selectivity for homologous lectin targets: differential targeting of the viral receptors L-SIGN and DC-SIGN Open
DC-SIGN and L-SIGN are C-type lectin receptors that act as viral coreceptors for SARS-CoV-2. Their distinct tissue localization justifies the search for a selective ligand for L-SIGN, despite their high homology suggesting the task hard to…
View article: Development of a highly selective glycomimetic ligand for L-SIGN: a new tool against SARS-CoV-2 and Ebola viruses
Development of a highly selective glycomimetic ligand for L-SIGN: a new tool against SARS-CoV-2 and Ebola viruses Open
DC-SIGN (CD209) and L‑SIGN (CD209L) are two C-type lectin receptors (CLRs) structurally homologous, but functionally rather different. DC-SIGN, expressed by dendritic cells and dedicated to recognizing carbohydrate molecular patterns assoc…
View article: Synthetic Zwitterionic <i>Streptococcus pneumoniae</i> Type 1 Oligosaccharides Carrying Labile <i>O</i>‐Acetyl Esters
Synthetic Zwitterionic <i>Streptococcus pneumoniae</i> Type 1 Oligosaccharides Carrying Labile <i>O</i>‐Acetyl Esters Open
We herein report the first total synthesis of the Streptococcus pneumoniae serotype 1 (Sp1) oligosaccharide, a unique zwitterionic capsular polysaccharide carrying labile O ‐acetyl esters. The target oligosaccharides, featuring rare α‐2,4‐…
View article: Synthetic Zwitterionic <i>Streptococcus pneumoniae</i> Type 1 Oligosaccharides Carrying Labile <i>O</i>‐Acetyl Esters
Synthetic Zwitterionic <i>Streptococcus pneumoniae</i> Type 1 Oligosaccharides Carrying Labile <i>O</i>‐Acetyl Esters Open
We herein report the first total synthesis of the Streptococcus pneumoniae serotype 1 (Sp1) oligosaccharide, a unique zwitterionic capsular polysaccharide carrying labile O ‐acetyl esters. The target oligosaccharides, featuring rare α‐2,4‐…
View article: Parent–infant closeness after preterm birth and depressive symptoms: A longitudinal study
Parent–infant closeness after preterm birth and depressive symptoms: A longitudinal study Open
Background Preterm birth increases the risk for postpartum depression in both mothers and fathers, calling for strategies to alleviate and prevent depressive symptoms in parents of preterm infants. The aim of this study was to assess the a…
View article: Petrophysical Characterization of Non-Magnetic Granites; Density and Magnetic Susceptibility Relationships
Petrophysical Characterization of Non-Magnetic Granites; Density and Magnetic Susceptibility Relationships Open
In this work we establish reliable correlations between density and magnetic susceptibility in three paramagnetic granites from the Pyrenees. In total, 128 sites (310 density measurements and >2600 susceptibility ones) were studied in the …
View article: Targeting transthyretin in Alzheimer's disease: Drug discovery of small-molecule chaperones as disease-modifying drug candidates for Alzheimer's disease
Targeting transthyretin in Alzheimer's disease: Drug discovery of small-molecule chaperones as disease-modifying drug candidates for Alzheimer's disease Open
Transthyretin (TTR) has a well-established role in neuroprotection in Alzheimer's Disease (AD). We have setup a drug discovery program of small-molecule compounds that act as chaperones enhancing TTR/Amyloid-beta peptide (Aβ) interactions.…
View article: pH modulates interaction of 14-3-3 proteins with pollen plasma membrane H+ ATPases independently from phosphorylation
pH modulates interaction of 14-3-3 proteins with pollen plasma membrane H+ ATPases independently from phosphorylation Open
Pollen grains transport the sperm cells through the style tissue via a fast-growing pollen tube to the ovaries where fertilization takes place. Pollen tube growth requires a precisely regulated network of cellular as well as molecular even…
View article: Targeting transthyretin in Alzheimer’s disease: drug discovery of small-molecule chaperones as disease-modifying drug candidates for Alzheimer’s disease
Targeting transthyretin in Alzheimer’s disease: drug discovery of small-molecule chaperones as disease-modifying drug candidates for Alzheimer’s disease Open
Transthyretin (TTR) has a well-established role in neuroprotection, evidenced in Alzheimer’s Disease (AD). By targeting TTR we have setup a drug discovery program of small-molecule compounds that act as chaperones, enhancing TTR/ amyloid-β…
View article: Targeting transthyretin in Alzheimer’s disease: drug discovery of small-molecule chaperones as disease-modifying drug candidates for Alzheimer’s disease
Targeting transthyretin in Alzheimer’s disease: drug discovery of small-molecule chaperones as disease-modifying drug candidates for Alzheimer’s disease Open
Transthyretin (TTR) has a well-established role in neuroprotection, evidenced in Alzheimer’s Disease (AD). By targeting TTR we have setup a drug discovery program of small-molecule compounds that act as chaperones, enhancing TTR/ amyloid-β…
View article: Selective <sup>13</sup>C‐Labels on Repeating Glycan Oligomers to Reveal Protein Binding Epitopes through NMR: Polylactosamine Binding to Galectins
Selective <sup>13</sup>C‐Labels on Repeating Glycan Oligomers to Reveal Protein Binding Epitopes through NMR: Polylactosamine Binding to Galectins Open
A combined chemo‐enzymatic synthesis/NMR‐based methodology is presented to identify, in unambiguous manner, the distinctive binding epitope within repeating sugar oligomers when binding to protein receptors. The concept is based on the inc…
View article: Selective <sup>13</sup>C‐Labels on Repeating Glycan Oligomers to Reveal Protein Binding Epitopes through NMR: Polylactosamine Binding to Galectins
Selective <sup>13</sup>C‐Labels on Repeating Glycan Oligomers to Reveal Protein Binding Epitopes through NMR: Polylactosamine Binding to Galectins Open
A combined chemo‐enzymatic synthesis/NMR‐based methodology is presented to identify, in unambiguous manner, the distinctive binding epitope within repeating sugar oligomers when binding to protein receptors. The concept is based on the inc…
View article: Cross-Linking Effects Dictate the Preference of Galectins to Bind LacNAc-Decorated HPMA Copolymers
Cross-Linking Effects Dictate the Preference of Galectins to Bind LacNAc-Decorated HPMA Copolymers Open
The interaction of multi-LacNAc (Galβ1-4GlcNAc)-containing N-(2-hydroxypropyl) methacrylamide (HPMA) copolymers with human galectin-1 (Gal-1) and the carbohydrate recognition domain (CRD) of human galectin-3 (Gal-3) was analyzed using NMR …
View article: Minimal epitope for Mannitou IgM on paucimannose-carrying glycoproteins
Minimal epitope for Mannitou IgM on paucimannose-carrying glycoproteins Open
\n Paucimannosidic glycans are restricted to the core structure [Man1–3GlcNAc2Fuc0–1] of N-glycans and are rarely found in mammalian tissues. Yet, especially [Man2-3GlcNAc2Fuc1] have been found significantly upregulated in tumors, includin…
View article: The two domains of human galectin-8 bind sialyl- and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR† † Electronic supplementary information (ESI) available. See DOI: 10.1039/d1cb00051a
The two domains of human galectin-8 bind sialyl- and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR† † Electronic supplementary information (ESI) available. See DOI: 10.1039/d1cb00051a Open
View article: Minimal epitope for Mannitou IgM on paucimannose-carrying glycoproteins
Minimal epitope for Mannitou IgM on paucimannose-carrying glycoproteins Open
Paucimannosidic glycans are restricted to the core structure [Man1–3GlcNAc2Fuc0–1] of N-glycans and are rarely found in mammalian tissues. Yet, especially [Man2-3GlcNAc2Fuc1] have been found significantly upregulated in tumors, including i…
View article: The two domains of human galectin-8 bind sialyl- and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR
The two domains of human galectin-8 bind sialyl- and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR Open
NMR, ITC, and MD data show that the two domains of human galectin-8 independently recognize sialyl- and fucosyl-containing glycans.
View article: Structure of a protective epitope reveals the importance of acetylation of <i>Neisseria meningitidis</i> serogroup A capsular polysaccharide
Structure of a protective epitope reveals the importance of acetylation of <i>Neisseria meningitidis</i> serogroup A capsular polysaccharide Open
Significance The elucidation of structural epitopes is key to understanding the mechanisms of action of vaccines. Here a multidisciplinary approach was used to map the glycan epitope of Neisseria meningitidis serogroup A capsular polysacch…
View article: Structural Characterization of N‐Linked Glycans in the Receptor Binding Domain of the SARS‐CoV‐2 Spike Protein and their Interactions with Human Lectins
Structural Characterization of N‐Linked Glycans in the Receptor Binding Domain of the SARS‐CoV‐2 Spike Protein and their Interactions with Human Lectins Open
The glycan structures of the receptor binding domain of the SARS‐CoV2 spike glycoprotein expressed in human HEK293F cells have been studied by using NMR. The different possible interacting epitopes have been deeply analysed and characteriz…