Ana Toplak
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A Sustainable Chemo-Enzymatic Approach to the Synthesis of Liraglutide Open
The development of green approaches to peptide synthesis is essential, as peptides are gaining prominence in the pharmaceutical market. Traditional methods often involve harmful solvents and high energy use, prompting a shift toward sustai…
Comparison of Graphic and Textual Biographies in Slovenian Literature Open
The paper compares two graphic biographies with traditional biographies of the same authors – renowned Slovenian writers Alma Karlin and Ivan Cankar. It compares the manner in which graphical and text-only biographies present the character…
Natural Occurring and Engineered Enzymes for Peptide Ligation and Cyclization Open
The renaissance of peptides as prospective therapeutics has fostered the development of novel strategies for their synthesis and modification. In this context, besides the development of new chemical peptide ligation approaches, especially…
Efficient Enzymatic Cyclization of Disulfide‐Rich Peptides by Using Peptide Ligases Open
Disulfide‐rich macrocyclic peptides—cyclotides, for example—represent a promising class of molecules with potential therapeutic use. Despite their potential their efficient synthesis at large scale still represents a major challenge. Here …
Design of a substrate-tailored peptiligase variant for the efficient synthesis of thymosin-α<sub>1</sub> Open
A substrate-tailored peptide ligase for the chemo-enzymatic peptide synthesis (CEPS) of thymosin-α1.
Omniligase‐1: A Powerful Tool for Peptide Head‐to‐Tail Cyclization Open
Strategies for the efficient synthesis of peptide macrocycles have been a long‐standing goal. In this paper, we demonstrate the use of the peptide ligase termed omniligase‐1 as a versatile and broadly applicable enzymatic tool for peptide …
Engineering a Diverse Ligase Toolbox for Peptide Segment Condensation Open
The substrate profile of peptiligase, a stable enzyme designed for peptide ligation in aqueous environments, was mapped using six different peptide libraries. The most discriminating substrate binding pocket proved to be the first nucleoph…
Peptiligase, an Enzyme for Efficient Chemoenzymatic Peptide Synthesis and Cyclization in Water Open
We describe a novel, organic cosolvent‐stable and cation‐independent engineered enzyme for peptide coupling reactions. The enzyme is a variant of a stable calcium‐independent mutant of subtilisin BPN′, with the catalytic Ser212 mutated to …
Discovery and engineering of enzymes for chemoenzymatic peptide synthesis Open
The use of peptides and proteins as therapeutic agents, nutritional additives or cosmetic ingredients is becoming more prominent. The number of therapeutic peptides in development is increasing, as well as their length, complexity and quan…